Restriction map for
pFN21AE5496
(Circular) (Six-base) MAPSORT of: pFN21AE5496.seq Check: 3523 from: 1 to: 5755
pFN21AE5496 5755 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 2326 3076 2326
Size: 750 5005
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3818 5202 5470 1632
Size: 2186 1384 268 1917
Fragments arranged by size:
2186 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 2813 4654 5631 1203
Size: 1610 1841 977 1327
Fragments arranged by size:
1841 1610 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 2420 1166
Size: 1254 4501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 3498 5070 3498
Size: 1572 4183
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2496 3064 3290 3442 5551 1493
Size: 1003 568 226 152 2109 1697
Fragments arranged by size:
2109 1697 1003 568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2326 3076 4531 618
Size: 325 217 627 539 750 1455 1842
Fragments arranged by size:
1842 1455 750 627 539 325 217
BanII G_rGCy'C
Cuts at: 1391 2268 2720 3074 1391
Size: 877 452 354 4072
Fragments arranged by size:
4072 877 452 354
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2153 3647 4857 2153
Size: 1494 1210 3051
Fragments arranged by size:
3051 1494 1210
BclI T'GATC_A
Cuts at: 1548 2940 1548
Size: 1392 4363
BfrBI ATG'CAT
Cuts at: 3022 3022
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2676 2904 3816 4972 1790
Size: 886 228 912 1156 2573
Fragments arranged by size:
2573 1156 912 886 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2927 1056
Size: 1871 3884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4415 1239
Size: 3176 2579
Bpu10I CC'TnA_GC
Cuts at: 2353 2353
Size: 5755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2493 2744 3880 4745 5043 2493
Size: 251 1136 865 298 3205
Fragments arranged by size:
3205 1136 865 298 251
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2780 4418 882
Size: 617 1281 1638 2219
Fragments arranged by size:
2219 1638 1281 617
BsaAI yAC'GTr
Cuts at: 493 1204 2938 3546 493
Size: 711 1734 608 2702
Fragments arranged by size:
2702 1734 711 608
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2308 2770 1186
Size: 1122 462 4171
Fragments arranged by size:
4171 1122 462
BseYI C'CCAG_C
Cuts at: 2086 2518 2675 2947 4958 5454 2086
Size: 432 157 272 2011 496 2387
Fragments arranged by size:
2387 2011 496 432 272 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2637 3816 3901 4972 2637
Size: 1179 85 1071 3420
Fragments arranged by size:
3420 1179 1071 85
BsmI GAATG_Cn'
Cuts at: 2031 3266 3359 2031
Size: 1235 93 4427
Fragments arranged by size:
4427 1235 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2420 2420
Size: 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2637 2676 2720 2904 3074 3816
Size: 399 478 369 39 44 184 170 742
Cuts at: 3816 3901 4972 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 399 369 184 170
85 44 39
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3110 844
Size: 2266 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2733 4250 4424 65
Size: 1183 1485 1517 174 1396
Fragments arranged by size:
1517 1485 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 2196 96
Size: 2100 3655
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2349 794
Size: 1555 4200
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2574 2422
Size: 152 5603
BstYI r'GATC_y
Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750
Size: 1113 367 385 17 1452 86 12 346
Cuts at: 5750 1972
Size: 1977
Fragments arranged by size:
1977 1452 1113 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2445 513
Size: 552 1380 3823
Fragments arranged by size:
3823 1380 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4065 4085 3271
Size: 794 20 4941
Fragments arranged by size:
4941 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DraIII CAC_nnn'GTG
Cuts at: 2189 2189
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2532 2646 3202
Size: 54 1425 50 275 18 702 114 556
Cuts at: 3202 4091 5720 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 702 556 275 114 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3685 1353
Size: 2332 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3818 4415 5202 5470 1239
Size: 393 2186 597 787 268 1524
Fragments arranged by size:
2186 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2496 3064 3442 2496
Size: 568 378 4809
Fragments arranged by size:
4809 568 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2093 2093
Size: 5755
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2074 2106 2569 2601 4396
Size: 32 135 32 502 32 463 32 1795
Cuts at: 4396 4428 4470 4502 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1795 502 463 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2012 3099 3345 669
Size: 988 281 74 1087 246 3079
Fragments arranged by size:
3079 1087 988 281 246 74
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 2330 3080 2330
Size: 750 5005
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2555 3066 4869 5053 1305
Size: 1250 511 1803 184 2007
Fragments arranged by size:
2007 1803 1250 511 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 2648 10
Size: 54 1425 343 702 114 3117
Fragments arranged by size:
3117 1425 702 343 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 2393 2420 3713 4072 4231 518
Size: 648 1143 84 27 1293 359 159 2042
Fragments arranged by size:
2042 1293 1143 648 359 159 84 27
MspA1I CmG'CkG
Cuts at: 1606 2420 3171 3848 4996 5241 1606
Size: 814 751 677 1148 245 2120
Fragments arranged by size:
2120 1148 814 751 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2445 513
Size: 552 1380 3823
Fragments arranged by size:
3823 1380 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 2923 1052
Size: 1871 3884
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NsiI A_TGCA'T
Cuts at: 3024 3024
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2661 2817 3026 3113 4658 1132
Size: 97 1432 156 209 87 1545 2229
Fragments arranged by size:
2229 1545 1432 209 156 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 2813 4654 2813
Size: 1841 3914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2110 1108
Size: 1002 4753
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 2100 3325 2100
Size: 1225 4530
PstI C_TGCA'G
Cuts at: 830 3107 830
Size: 2277 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2420 2420
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2686 3103 4238 4919 5110 826
Size: 219 1641 417 1135 681 191 1471
Fragments arranged by size:
1641 1471 1135 681 417 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2508 2759 3859 4760 5022 820
Size: 197 926 565 251 1100 901 262 1553
Fragments arranged by size:
1553 1100 926 901 565 262 251 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2445 3194 513
Size: 552 1380 749 3074
Fragments arranged by size:
3074 1380 749 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2927 3782 3799 3952 4137 4582 325
Size: 1255 1347 855 17 153 185 445 1498
Fragments arranged by size:
1498 1347 1255 855 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 2844 4001
Size: 274 80 33 51 1443 219 648 1157
Cuts at: 4001 5713 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1157 648 274 219 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2944 2976 5715 5747 2944
Size: 32 2739 32 2952
Fragments arranged by size:
2952 2739 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI