Restriction map for
pFN21AE5493
(Circular) (Six-base) MAPSORT of: pFN21AE5493.seq Check: 6921 from: 1 to: 5746
pFN21AE5493 5746 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3809 5193 5461 1632
Size: 2177 1384 268 1917
Fragments arranged by size:
2177 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 2076 4645 5622 1203
Size: 873 2569 977 1327
Fragments arranged by size:
2569 1327 977 873
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 3489 5061 3489
Size: 1572 4174
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 2304 2421 2543 2978 3055 3281 3433 5542
Size: 811 117 122 435 77 226 152 2109
Cuts at: 5542 1493
Size: 1697
Fragments arranged by size:
2109 1697 811 435 226 152 122 117
77
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 2661 3071 1870
Size: 73 574 144 410 4545
Fragments arranged by size:
4545 574 410 144 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2220 2253 2220
Size: 33 5713
BamHI G'GATC_C
Cuts at: 2451 3076 3443 2451
Size: 625 367 4754
Fragments arranged by size:
4754 625 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3067 4522 618
Size: 325 217 627 1280 1455 1842
Fragments arranged by size:
1842 1455 1280 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2462 3065 1391
Size: 1071 603 4072
Fragments arranged by size:
4072 1071 603
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4775
BbvCI CC'TCA_GC
Cuts at: 2192 2192
Size: 5746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 2797 1548
Size: 1249 4497
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4357 136
Size: 107 122 71 631 605 2685 1525
Fragments arranged by size:
2685 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2913 5741 2913
Size: 2828 2918
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 3807 4963 1790
Size: 2017 1156 2573
Fragments arranged by size:
2573 2017 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2715 4406 1239
Size: 1476 1691 2579
Fragments arranged by size:
2579 1691 1476
Bpu10I CC'TnA_GC
Cuts at: 2192 2192
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 4409 882
Size: 617 1021 1889 2219
Fragments arranged by size:
2219 1889 1021 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 3935 4570 275
Size: 53 83 186 936 2020 382 635 1451
Fragments arranged by size:
2020 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2180 1186
Size: 949 45 4752
Fragments arranged by size:
4752 949 45
BseYI C'CCAG_C
Cuts at: 2163 4949 5445 2163
Size: 2786 496 2464
Fragments arranged by size:
2786 2464 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2212 1163
Size: 573 476 4697
Fragments arranged by size:
4697 573 476
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3807 3892 4963 3807
Size: 85 1071 4590
Fragments arranged by size:
4590 1071 85
BsmI GAATG_Cn'
Cuts at: 2903 3257 3350 2903
Size: 354 93 5299
Fragments arranged by size:
5299 354 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2462 3065 3807 3892 4963 1391
Size: 399 672 603 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 672 603 399 85
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 2409 2907 3635 5365 2409
Size: 498 728 1730 2790
Fragments arranged by size:
2790 1730 728 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 844
Size: 2257 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2578 2646 3039 4241 4415 65
Size: 1183 1330 68 393 1202 174 1396
Fragments arranged by size:
1396 1330 1202 1183 393 174 68
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 2778 3036 96
Size: 2682 258 2806
Fragments arranged by size:
2806 2682 258
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 2284
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 2451 2690 2913 3076 3443 3828 3845 5297
Size: 479 239 223 163 367 385 17 1452
Cuts at: 5297 5383 5395 5741 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 479 385 367 346 239 223
163 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4255
Fragments arranged by size:
4255 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 3262 4056 4076 3262
Size: 794 20 4932
Fragments arranged by size:
4932 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3193 4082 5711
Size: 54 1425 50 275 18 1363 889 1629
Cuts at: 5711 8
Size: 43
Fragments arranged by size:
1629 1425 1363 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2586 3676 1353
Size: 737 496 1090 3423
Fragments arranged by size:
3423 1090 737 496
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2715 3809 4406 5193 5461 1239
Size: 393 1083 1094 597 787 268 1524
Fragments arranged by size:
1524 1094 1083 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2543 3055 3433 2543
Size: 512 378 4856
Fragments arranged by size:
4856 512 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 5746
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4387 4419 4461 4493 1373
Size: 32 135 32 2815 32 42 32 2626
Fragments arranged by size:
2815 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2712 748
Size: 1964 3782
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 4860 5044 1305
Size: 753 2802 184 2007
Fragments arranged by size:
2802 2007 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3924
Fragments arranged by size:
3924 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2282 2684 2744 2852 3704 4063
Size: 648 1011 105 402 60 108 852 359
Cuts at: 4063 4222 518
Size: 159 2042
Fragments arranged by size:
2042 1011 852 648 402 359 159 108
105 60
MspA1I CmG'CkG
Cuts at: 1606 3162 3839 4987 5232 1606
Size: 1556 677 1148 245 2120
Fragments arranged by size:
2120 1556 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4255
Fragments arranged by size:
4255 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4844
PfoI T'CCnGG_A
Cuts at: 2447 2447
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspXI vC'TCGA_Gb
Cuts at: 2661 2661
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2463 2484 2872 3094 4229 4910 5101
Size: 219 1418 21 388 222 1135 681 191
Cuts at: 5101 826
Size: 1471
Fragments arranged by size:
1471 1418 1135 681 388 222 219 191
21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2661 3850 4751 5013 820
Size: 197 926 718 1189 901 262 1553
Fragments arranged by size:
1553 1189 926 901 718 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 2586 3670 5
Size: 47 2534 1084 2081
Fragments arranged by size:
2534 2081 1084 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2004 2047 3185 513
Size: 552 939 43 1138 3074
Fragments arranged by size:
3074 1138 939 552 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 3773 3790 3943 4128 4573 325
Size: 1255 597 1596 17 153 185 445 1498
Fragments arranged by size:
1596 1498 1255 597 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2754 2778
Size: 274 80 33 51 1443 204 573 24
Cuts at: 2778 3036 3992 5704 96
Size: 258 956 1712 138
Fragments arranged by size:
1712 1443 956 573 274 258 204 138
80 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2064 2096 5706 5738 2064
Size: 32 3610 32 2072
Fragments arranged by size:
3610 2072 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 2661 1943
Size: 718 5028
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 3875 2340
Size: 1535 4211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI