Restriction map for
pFN21AE5487
(Circular) (Six-base) MAPSORT of: pFN21AE5487.seq Check: 8999 from: 1 to: 5725
pFN21AE5487 5725 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2136 2792 2880 3788 5172 5440 1632
Size: 491 13 656 88 908 1384 268 1917
Fragments arranged by size:
1917 1384 908 656 491 268 88 13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 2813 4624 5601 1203
Size: 1610 1811 977 1327
Fragments arranged by size:
1811 1610 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 2068 4454 2068
Size: 2386 3339
AleI CACnn'nnGTG
Cuts at: 1166 2933 1166
Size: 1767 3958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 3468 5040 3468
Size: 1572 4153
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 3050 1870
Size: 73 1107 4545
Fragments arranged by size:
4545 1107 73
BamHI G'GATC_C
Cuts at: 2603 3055 3422 2603
Size: 452 367 4906
Fragments arranged by size:
4906 452 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3046 4501 618
Size: 325 217 627 1259 1455 1842
Fragments arranged by size:
1842 1455 1259 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3044 1391
Size: 1653 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2161 928
Size: 971 262 4492
Fragments arranged by size:
4492 971 262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 2455 1548
Size: 907 4818
BfrBI ATG'CAT
Cuts at: 2779 2779
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 3786 4942 1790
Size: 1996 1156 2573
Fragments arranged by size:
2573 1996 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3456 4414 442
Size: 416 53 83 399 24 2039 958 1753
Fragments arranged by size:
2039 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2853 4385 1239
Size: 1614 1532 2579
Fragments arranged by size:
2579 1614 1532
Bpu10I CC'TnA_GC
Cuts at: 2787 2787
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3850 4715 5013 3850
Size: 865 298 4562
Fragments arranged by size:
4562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2869 4388 882
Size: 617 1370 1519 2219
Fragments arranged by size:
2219 1519 1370 617
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3532 3914 4549 275
Size: 53 83 186 936 1999 382 635 1451
Fragments arranged by size:
1999 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4158 4556 4830 4977 5388 4158
Size: 398 274 147 411 4495
Fragments arranged by size:
4495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5500
Fragments arranged by size:
5500 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2631 1186
Size: 1445 4280
BseYI C'CCAG_C
Cuts at: 4928 5424 4928
Size: 496 5229
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3786 3871 4942 3786
Size: 85 1071 4569
Fragments arranged by size:
4569 1071 85
BsmI GAATG_Cn'
Cuts at: 2779 3236 3329 2779
Size: 457 93 5175
Fragments arranged by size:
5175 457 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3044 3786 3871 4942 1391
Size: 399 1254 742 85 1071 2174
Fragments arranged by size:
2174 1254 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2049 3080 844
Size: 1205 1031 3489
Fragments arranged by size:
3489 1205 1031
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2451 4220 4394 65
Size: 1183 1203 1769 174 1396
Fragments arranged by size:
1769 1396 1203 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 2603 3055 3422 3807 3824 5276 5362 5374
Size: 631 452 367 385 17 1452 86 12
Cuts at: 5374 5720 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 631 452 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4234
Fragments arranged by size:
4234 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 3000 3241 4035 4055 2529
Size: 471 241 794 20 4199
Fragments arranged by size:
4199 794 471 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3172 4061 5690
Size: 54 1425 50 275 18 1342 889 1629
Cuts at: 5690 8
Size: 43
Fragments arranged by size:
1629 1425 1342 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2696 3655 1353
Size: 1343 959 3423
Fragments arranged by size:
3423 1343 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2136 2792 2853 2880 3788 4385
Size: 393 491 13 656 61 27 908 597
Cuts at: 4385 5172 5440 1239
Size: 787 268 1524
Fragments arranged by size:
1524 908 787 656 597 491 393 268
61 27 13
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 4872 1678
Size: 3194 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 2830 2862 4366
Size: 32 135 32 482 32 744 32 1504
Cuts at: 4366 4398 4440 4472 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1504 744 482 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 2931 3324 2931
Size: 393 5332
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4839 5023 1305
Size: 3534 184 2007
Fragments arranged by size:
3534 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3903
Fragments arranged by size:
3903 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2912 2933 3683 4042 4201 518
Size: 648 1746 21 750 359 159 2042
Fragments arranged by size:
2042 1746 750 648 359 159 21
MspA1I CmG'CkG
Cuts at: 1606 3141 3818 4966 5211 1606
Size: 1535 677 1148 245 2120
Fragments arranged by size:
2120 1535 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4234
Fragments arranged by size:
4234 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NsiI A_TGCA'T
Cuts at: 2781 2781
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2503 2561 2624 2817 3083 4628 1132
Size: 97 1274 58 63 193 266 1545 2229
Fragments arranged by size:
2229 1545 1274 266 193 97 63 58
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 2813 4624 2813
Size: 1811 3914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2898 3794 3826 5307 5339 2866
Size: 32 896 32 1481 32 3252
Fragments arranged by size:
3252 1481 896 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2117 1613
Size: 504 5221
PsiI TTA'TAA
Cuts at: 2037 3295 2037
Size: 1258 4467
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2316 2421 2962 3073 4208 4889 5080
Size: 219 1271 105 541 111 1135 681 191
Cuts at: 5080 826
Size: 1471
Fragments arranged by size:
1471 1271 1135 681 541 219 191 111
105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3829 4730 4992 820
Size: 197 926 1886 901 262 1553
Fragments arranged by size:
1886 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 2922 3649 5
Size: 47 2870 727 2081
Fragments arranged by size:
2870 2081 727 47
StuI AGG'CCT
Cuts at: 1517 2804 1517
Size: 1287 4438
StyI C'CwwG_G
Cuts at: 513 1065 2004 3164 513
Size: 552 939 1160 3074
Fragments arranged by size:
3074 1160 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2521 3752 3769 3922 4107 4552 325
Size: 1255 941 1231 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1231 941 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5685 5717 5685
Size: 32 5693
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 2430 3854 2430
Size: 1424 4301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI