Restriction map for
pFN21AE5486
(Circular) (Six-base) MAPSORT of: pFN21AE5486.seq Check: 4193 from: 1 to: 5722
pFN21AE5486 5722 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2567 2567
Size: 5722
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 1937
Size: 1128 4594
Acc65I G'GTAC_C
Cuts at: 2832 3043 2832
Size: 211 5511
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2865 3785 5169 5437 1632
Size: 1233 920 1384 268 1917
Fragments arranged by size:
1917 1384 1233 920 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 4621 5598 1203
Size: 3418 977 1327
Fragments arranged by size:
3418 1327 977
AgeI A'CCGG_T
Cuts at: 2421 4553 2421
Size: 2132 3590
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5722
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2818 1184
Size: 1634 4088
AlwNI CAG_nnn'CTG
Cuts at: 3465 5037 3465
Size: 1572 4150
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 2090 3031 3257 3409 5518 1493
Size: 597 941 226 152 2109 1697
Fragments arranged by size:
2109 1697 941 597 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 3047 1870
Size: 73 1104 4545
Fragments arranged by size:
4545 1104 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2824 2857 2824
Size: 33 5689
BamHI G'GATC_C
Cuts at: 2116 2697 3052 3419 2116
Size: 581 355 367 4419
Fragments arranged by size:
4419 581 367 355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2666 2832 3043 4498 618
Size: 325 217 627 879 166 211 1455 1842
Fragments arranged by size:
1842 1455 879 627 325 217 211 166
BanII G_rGCy'C
Cuts at: 1391 3041 1391
Size: 1650 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4751
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3614 4824 3614
Size: 1210 4512
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2481 4333 136
Size: 107 122 71 631 605 809 1852 1525
Fragments arranged by size:
1852 1525 809 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5717 5717
Size: 5722
BlpI GC'TnA_GC
Cuts at: 3139 3139
Size: 5722
Bme1580I G_kGCm'C
Cuts at: 1790 2959 3783 4939 1790
Size: 1169 824 1156 2573
Fragments arranged by size:
2573 1169 1156 824
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2521 3453 4411
Size: 416 53 83 399 24 1104 932 958
Cuts at: 4411 442
Size: 1753
Fragments arranged by size:
1753 1104 958 932 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4382 1239
Size: 3143 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3847 4712 5010 3847
Size: 865 298 4559
Fragments arranged by size:
4559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4385 882
Size: 617 2886 2219
Fragments arranged by size:
2886 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 5716 5716
Size: 5722
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3529 3911 4546 275
Size: 53 83 186 936 1996 382 635 1451
Fragments arranged by size:
1996 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2421 4155 4553 4827 4974 5385 2421
Size: 1734 398 274 147 411 2758
Fragments arranged by size:
2758 1734 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5497
Fragments arranged by size:
5497 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2600 1186
Size: 901 513 4308
Fragments arranged by size:
4308 901 513
BseYI C'CCAG_C
Cuts at: 2141 2176 4925 5421 2141
Size: 35 2749 496 2442
Fragments arranged by size:
2749 2442 496 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 2983 1163
Size: 573 696 551 3902
Fragments arranged by size:
3902 696 573 551
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3172 3933 4961 1540
Size: 275 186 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3783 3868 4939 3783
Size: 85 1071 4566
Fragments arranged by size:
4566 1071 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2959 3041 3783 3868 4939 1391
Size: 399 1169 82 742 85 1071 2174
Fragments arranged by size:
2174 1169 1071 742 399 85 82
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 2463 3611 5341 2463
Size: 1148 1730 2844
Fragments arranged by size:
2844 1730 1148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 2567 3077 844
Size: 1349 374 510 3489
Fragments arranged by size:
3489 1349 510 374
BsrBI CCG'CTC
Cuts at: 1394 1823 3609 1394
Size: 429 1786 3507
Fragments arranged by size:
3507 1786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4217 4391 65
Size: 1183 2969 174 1396
Fragments arranged by size:
2969 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2131 2421 4366 4553 1700
Size: 110 321 290 1945 187 2869
Fragments arranged by size:
2869 1945 321 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 2889 96
Size: 2793 2929
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 3782 4794 3782
Size: 1012 4710
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 2899 1184
Size: 1715 4007
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 2164
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2116 2697 3052 3419 3804 3821 5273 5359
Size: 144 581 355 367 385 17 1452 86
Cuts at: 5359 5371 5717 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 581 385 367 355 346 144
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2618 2933 2618
Size: 315 5407
BtgI C'CryG_G
Cuts at: 513 1065 2286 513
Size: 552 1221 3949
Fragments arranged by size:
3949 1221 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4983
Fragments arranged by size:
4983 683 56
BtsI GCAGTG_nn'
Cuts at: 3238 4032 4052 3238
Size: 794 20 4908
Fragments arranged by size:
4908 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2359 4729 809
Size: 507 1043 2370 1802
Fragments arranged by size:
2370 1802 1043 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2430 3169 4058
Size: 54 1425 50 275 18 600 739 889
Cuts at: 4058 5687 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 739 600 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2861 3652 1353
Size: 1508 791 3423
Fragments arranged by size:
3423 1508 791
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4309 4683 4829 387
Size: 1064 138 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2865 3785 4382 5169 5437 1239
Size: 393 1233 920 597 787 268 1524
Fragments arranged by size:
1524 1233 920 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2691 2691
Size: 5722
EcoRI G'AATT_C
Cuts at: 2090 3031 3409 2090
Size: 941 378 4403
Fragments arranged by size:
4403 941 378
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 4869 1678
Size: 3191 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4363 4395 4437 4469 1373
Size: 32 135 32 2791 32 42 32 2626
Fragments arranged by size:
2791 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 3312 669
Size: 988 281 1128 246 3079
Fragments arranged by size:
3079 1128 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5722
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 2836 3047 2836
Size: 211 5511
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 2652 2768 4836 5020 1305
Size: 969 378 116 2068 184 2007
Fragments arranged by size:
2068 2007 969 378 184 116
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2432 10
Size: 54 1425 343 600 3300
Fragments arranged by size:
3300 1425 600 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2906 3680 4039 4198 518
Size: 648 1065 675 774 359 159 2042
Fragments arranged by size:
2042 1065 774 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2846 3138 3815 4963 5208 1606
Size: 1240 292 677 1148 245 2120
Fragments arranged by size:
2120 1240 1148 677 292 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5170
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5722
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 2314 2385 2398 3080 4625 1132
Size: 97 1085 71 13 682 1545 2229
Fragments arranged by size:
2229 1545 1085 682 97 71 13
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5722
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PstI C_TGCA'G
Cuts at: 830 2130 3074 830
Size: 1300 944 3478
Fragments arranged by size:
3478 1300 944
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
PvuII CAG'CTG
Cuts at: 2846 2846
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SexAI A'CCwGG_T
Cuts at: 2163 2163
Size: 5722
SfcI C'TryA_G
Cuts at: 826 1045 2126 2926 3070 4205 4886 5077 826
Size: 219 1081 800 144 1135 681 191 1471
Fragments arranged by size:
1471 1135 1081 800 681 219 191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3826 4727 4989 820
Size: 197 926 1883 901 262 1553
Fragments arranged by size:
1883 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5722
StyI C'CwwG_G
Cuts at: 513 1065 2259 3161 513
Size: 552 1194 902 3074
Fragments arranged by size:
3074 1194 902 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2654 3749 3766 3919 4104 4549 325
Size: 1255 1074 1095 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1095 1074 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 2889 3968
Size: 274 80 33 51 1443 258 654 1079
Cuts at: 3968 5680 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1079 654 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5682 5714 5682
Size: 32 5690
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2302 2869 2257
Size: 45 567 5110
Fragments arranged by size:
5110 567 45
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5722
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 2918 3851 2918
Size: 933 4789
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI