Restriction map for
pFN21AE5485
(Circular) (Six-base) MAPSORT of: pFN21AE5485.seq Check: 4897 from: 1 to: 5719
pFN21AE5485 5719 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3529 278
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3062 1937
Size: 1125 4594
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2497 2963 3782 5166 5434 1632
Size: 708 157 466 819 1384 268 1917
Fragments arranged by size:
1917 1384 819 708 466 268 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 2856 4618 5595 1203
Size: 1653 1762 977 1327
Fragments arranged by size:
1762 1653 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AlwNI CAG_nnn'CTG
Cuts at: 3462 5034 3462
Size: 1572 4147
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 2365 3028 3254 3406 5515 1493
Size: 872 663 226 152 2109 1697
Fragments arranged by size:
2109 1697 872 663 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 3044 1870
Size: 73 1101 4545
Fragments arranged by size:
4545 1101 73
BamHI G'GATC_C
Cuts at: 2226 3049 3416 2226
Size: 823 367 4529
Fragments arranged by size:
4529 823 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3040 4495 618
Size: 325 217 627 1253 1455 1842
Fragments arranged by size:
1842 1455 1253 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3038 1391
Size: 1647 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 928
Size: 971 429 4319
Fragments arranged by size:
4319 971 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3910 3944 1903
Size: 34 50 34 1889 34 3678
Fragments arranged by size:
3678 1889 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2257 3611 4821 2257
Size: 1354 1210 3155
Fragments arranged by size:
3155 1354 1210
BclI T'GATC_A
Cuts at: 1548 2667 1548
Size: 1119 4600
BfrBI ATG'CAT
Cuts at: 2339 2339
Size: 5719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 3780 4936 1790
Size: 1990 1156 2573
Fragments arranged by size:
2573 1990 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3450 4408 442
Size: 416 53 83 399 24 2033 958 1753
Fragments arranged by size:
2033 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2010 4379 1239
Size: 771 2369 2579
Fragments arranged by size:
2579 2369 771
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2693 3844 4709 5007 2693
Size: 1151 865 298 3405
Fragments arranged by size:
3405 1151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3526 3908 4543 275
Size: 53 83 186 936 1993 382 635 1451
Fragments arranged by size:
1993 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4152 4550 4824 4971 5382 4152
Size: 398 274 147 411 4489
Fragments arranged by size:
4489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5494
Fragments arranged by size:
5494 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2569 2652 1186
Size: 1383 83 4253
Fragments arranged by size:
4253 1383 83
BseYI C'CCAG_C
Cuts at: 2759 4922 5418 2759
Size: 2163 496 3060
Fragments arranged by size:
3060 2163 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2527 1163
Size: 573 791 4355
Fragments arranged by size:
4355 791 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3780 3865 4936 3780
Size: 85 1071 4563
Fragments arranged by size:
4563 1071 85
BsmI GAATG_Cn'
Cuts at: 2339 3230 3323 2339
Size: 891 93 4735
Fragments arranged by size:
4735 891 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3038 3780 3865 4936 1391
Size: 399 1248 742 85 1071 2174
Fragments arranged by size:
2174 1248 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 2271 3608 5338 2271
Size: 1337 1730 2652
Fragments arranged by size:
2652 1730 1337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3074 844
Size: 2230 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2151 2743 4214 4388 65
Size: 1183 903 592 1471 174 1396
Fragments arranged by size:
1471 1396 1183 903 592 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2529 794
Size: 1735 3984
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstXI CCAn_nnnn'nTGG
Cuts at: 3009 3009
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 2226 3049 3416 3801 3818 5270 5356 5368
Size: 254 823 367 385 17 1452 86 12
Cuts at: 5368 5714 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 823 385 367 346 254 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4980
Fragments arranged by size:
4980 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 4029 4049 3235
Size: 794 20 4905
Fragments arranged by size:
4905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3166 4055 5684
Size: 54 1425 50 275 18 1336 889 1629
Cuts at: 5684 8
Size: 43
Fragments arranged by size:
1629 1425 1336 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2956 3649 1353
Size: 1603 693 3423
Fragments arranged by size:
3423 1603 693
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2010 2340 2497 2963 3782 4379 5166
Size: 393 378 330 157 466 819 597 787
Cuts at: 5166 5434 1239
Size: 268 1524
Fragments arranged by size:
1524 819 787 597 466 393 378 330
268 157
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 4866 1678
Size: 3188 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2644 2676 2689 2721 4360
Size: 32 135 32 1072 32 13 32 1639
Cuts at: 4360 4392 4434 4466 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1639 1072 135 42 32 32 32
32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3063 3309 669
Size: 988 281 1125 246 3079
Fragments arranged by size:
3079 1125 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2607 2775 2877 4833 5017 1305
Size: 1302 168 102 1956 184 2007
Fragments arranged by size:
2007 1956 1302 184 168 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3897
Fragments arranged by size:
3897 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3677 4036 4195 518
Size: 648 2511 359 159 2042
Fragments arranged by size:
2511 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2186 3135 3812 4960 5205 1606
Size: 580 949 677 1148 245 2120
Fragments arranged by size:
2120 1148 949 677 580 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5167
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NsiI A_TGCA'T
Cuts at: 2341 2341
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2183 2527 2860 3077 4622 1132
Size: 97 954 344 333 217 1545 2229
Fragments arranged by size:
2229 1545 954 344 333 217 97
PasI CC'CwG_GG
Cuts at: 1348 2951 1348
Size: 1603 4116
PciI A'CATG_T
Cuts at: 2856 4618 2856
Size: 1762 3957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PstI C_TGCA'G
Cuts at: 830 2080 3071 830
Size: 1250 991 3478
Fragments arranged by size:
3478 1250 991
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2186 2186
Size: 5719
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SapI GCTCTTCn'nnn_
Cuts at: 2956 2956
Size: 5719
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SfcI C'TryA_G
Cuts at: 826 1045 2076 3067 4202 4883 5074 826
Size: 219 1031 991 1135 681 191 1471
Fragments arranged by size:
1471 1135 1031 991 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2708 3823 4724 4986 820
Size: 197 926 765 1115 901 262 1553
Fragments arranged by size:
1553 1115 926 901 765 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 3158 513
Size: 552 2093 3074
Fragments arranged by size:
3074 2093 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 3746 3763 3916 4101 4546 325
Size: 1255 1035 1131 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1131 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3965 5677 96
Size: 274 80 33 51 1443 1988 1712 138
Fragments arranged by size:
1988 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 2193 3848 2193
Size: 1655 4064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3527 276
Size: 53 83 186 936 1993 2468
Fragments arranged by size:
2468 1993 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI