Restriction map for
pFN21AE5482
(Circular) (Six-base) MAPSORT of: pFN21AE5482.seq Check: 4884 from: 1 to: 5710
pFN21AE5482 5710 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2807 2807
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2113 2615 3773 5157 5425 1632
Size: 481 502 1158 1384 268 1917
Fragments arranged by size:
1917 1384 1158 502 481 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 2492 2733 4609 5586 1203
Size: 1289 241 1876 977 1327
Fragments arranged by size:
1876 1327 1289 977 241
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 2497 1166
Size: 1331 4379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2483 1184
Size: 1299 4411
AlwNI CAG_nnn'CTG
Cuts at: 2638 3453 5025 2638
Size: 815 1572 3323
Fragments arranged by size:
3323 1572 815
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2981 3019 3245 3397 5506 1493
Size: 1488 38 226 152 2109 1697
Fragments arranged by size:
2109 1697 1488 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2592 3035 1870
Size: 73 649 443 4545
Fragments arranged by size:
4545 649 443 73
AvrII C'CTAG_G
Cuts at: 2266 2266
Size: 5710
BamHI G'GATC_C
Cuts at: 2012 3040 3407 2012
Size: 1028 367 4315
Fragments arranged by size:
4315 1028 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3031 4486 618
Size: 325 217 627 1244 1455 1842
Fragments arranged by size:
1842 1455 1244 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2148 3029 1391
Size: 757 881 4072
Fragments arranged by size:
4072 881 757
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3901 3935 1903
Size: 34 50 34 1880 34 3678
Fragments arranged by size:
3678 1880 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 2325 1548
Size: 777 4933
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 3771 4927 1790
Size: 1981 1156 2573
Fragments arranged by size:
2573 1981 1156
BmgBI CAC'GTC
Cuts at: 1318 2495 1318
Size: 1177 4533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4370 1239
Size: 3131 2579
Bpu10I CC'TnA_GC
Cuts at: 2877 2877
Size: 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2387 1186
Size: 961 240 4509
Fragments arranged by size:
4509 961 240
BseYI C'CCAG_C
Cuts at: 2376 4913 5409 2376
Size: 2537 496 2677
Fragments arranged by size:
2677 2537 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 3771 3856 4927 2148
Size: 1623 85 1071 2931
Fragments arranged by size:
2931 1623 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 3029 3771 3856 4927 1391
Size: 399 358 881 742 85 1071 2174
Fragments arranged by size:
2174 1071 881 742 399 358 85
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2807 3065 844
Size: 1963 258 3489
Fragments arranged by size:
3489 1963 258
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2012 3040 3407 3792 3809 5261 5347 5359
Size: 40 1028 367 385 17 1452 86 12
Cuts at: 5359 5705 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1028 385 367 346 86 40
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2070 2430 513
Size: 552 1005 360 3793
Fragments arranged by size:
3793 1005 552 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2427 3157 4046
Size: 54 1425 50 275 18 597 730 889
Cuts at: 4046 5675 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 730 597 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 3640 1353
Size: 812 1475 3423
Fragments arranged by size:
3423 1475 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2146
Size: 5710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2113 2615 3773 4370 5157 5425 1239
Size: 393 481 502 1158 597 787 268 1524
Fragments arranged by size:
1524 1158 787 597 502 481 393 268
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
EcoRV GAT'ATC
Cuts at: 2673 2673
Size: 5710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 5710
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 4857 1678
Size: 3179 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2221 2253 2929 2961 4351
Size: 32 135 32 649 32 676 32 1390
Cuts at: 4351 4383 4425 4457 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1390 676 649 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2602 3054 3300 669
Size: 988 281 664 452 246 3079
Fragments arranged by size:
3079 988 664 452 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2252 2497 2899 2921 3668 4027
Size: 648 1059 27 245 402 22 747 359
Cuts at: 4027 4186 518
Size: 159 2042
Fragments arranged by size:
2042 1059 747 648 402 359 245 159
27 22
MspA1I CmG'CkG
Cuts at: 1606 3126 3803 4951 5196 1606
Size: 1520 677 1148 245 2120
Fragments arranged by size:
2120 1520 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2070 2430 513
Size: 552 1005 360 3793
Fragments arranged by size:
3793 1005 552 360
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2737 3068 4613 1132
Size: 97 1508 331 1545 2229
Fragments arranged by size:
2229 1545 1508 331 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 2733 4609 2733
Size: 1876 3834
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2804 3062 830
Size: 1974 258 3478
Fragments arranged by size:
3478 1974 258
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
SacI G_AGCT'C
Cuts at: 2148 2148
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2800 3058 4193 4874 5065 826
Size: 219 1755 258 1135 681 191 1471
Fragments arranged by size:
1755 1471 1135 681 258 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3814 4715 4977 820
Size: 197 926 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3001 3634 5
Size: 47 2949 633 2081
Fragments arranged by size:
2949 2081 633 47
StuI AGG'CCT
Cuts at: 1517 2789 1517
Size: 1272 4438
StyI C'CwwG_G
Cuts at: 513 1065 2070 2266 2316 2430 3149 513
Size: 552 1005 196 50 114 719 3074
Fragments arranged by size:
3074 1005 719 552 196 114 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 3737 3754 3907 4092 4537 325
Size: 1255 483 1674 17 153 185 445 1498
Fragments arranged by size:
1674 1498 1255 483 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2501 3956 5668
Size: 274 80 33 51 1443 524 1455 1712
Cuts at: 5668 96
Size: 138
Fragments arranged by size:
1712 1455 1443 524 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 2986 3046 2986
Size: 60 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 2789 3839 2789
Size: 1050 4660
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI