Restriction map for
pFN21AE5478
(Circular) (Six-base) MAPSORT of: pFN21AE5478.seq Check: 7924 from: 1 to: 5695
pFN21AE5478 5695 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2473 2627 3758 5142 5410 1632
Size: 841 154 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1131 841 268 154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 4594 5571 1203
Size: 3391 977 1327
Fragments arranged by size:
3391 1327 977
AgeI A'CCGG_T
Cuts at: 2415 4526 2415
Size: 2111 3584
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 2128 3438 5010 2128
Size: 1310 1572 2813
Fragments arranged by size:
2813 1572 1310
ApaLI G'TGCA_C
Cuts at: 3752 4908 3752
Size: 1156 4539
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 3020 1870
Size: 73 1077 4545
Fragments arranged by size:
4545 1077 73
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3016 4471 618
Size: 325 217 627 1229 1455 1842
Fragments arranged by size:
1842 1455 1229 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2953 3014 1391
Size: 1562 61 4072
Fragments arranged by size:
4072 1562 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3886 3920 1903
Size: 34 1949 34 3678
Fragments arranged by size:
3678 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2507 4306 136
Size: 107 122 71 631 605 835 1799 1525
Fragments arranged by size:
1799 1525 835 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2260 5690 2260
Size: 3430 2265
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2512 3756 4912 1790
Size: 722 1244 1156 2573
Fragments arranged by size:
2573 1244 1156 722
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5695
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2566 3426 4384
Size: 416 53 83 399 24 1149 860 958
Cuts at: 4384 442
Size: 1753
Fragments arranged by size:
1753 1149 958 860 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4355 1239
Size: 3116 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2274 2741 2947 3820 4685 4983 2274
Size: 467 206 873 865 298 2986
Fragments arranged by size:
2986 873 865 467 298 206
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3884 4519 275
Size: 53 83 186 936 1969 382 635 1451
Fragments arranged by size:
1969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2415 4128 4526 4800 4947 5358 2415
Size: 1713 398 274 147 411 2752
Fragments arranged by size:
2752 1713 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5695
BseYI C'CCAG_C
Cuts at: 4898 5394 4898
Size: 496 5199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3145 3906 4934 1540
Size: 275 186 1144 761 1028 2301
Fragments arranged by size:
2301 1144 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3756 3841 4912 3756
Size: 85 1071 4539
Fragments arranged by size:
4539 1071 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2512 2953 3014 3756 3841 4912 1391
Size: 399 722 441 61 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 722 441 399 85 61
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 2457 3584 5314 2457
Size: 1127 1730 2838
Fragments arranged by size:
2838 1730 1127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2983 3050 844
Size: 2139 67 3489
Fragments arranged by size:
3489 2139 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 1394
Size: 429 1759 3507
Fragments arranged by size:
3507 1759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2415 4339 4526 1700
Size: 110 605 1924 187 2869
Fragments arranged by size:
2869 1924 605 187 110
BsrGI T'GTAC_A
Cuts at: 96 2378 96
Size: 2282 3413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5695
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 2124 2260 3025 3392 3777 3794 5246 5332
Size: 152 136 765 367 385 17 1452 86
Cuts at: 5332 5344 5690 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 765 385 367 346 152 136
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 2406 3211 4005 4025 2406
Size: 805 794 20 4076
Fragments arranged by size:
4076 805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2643 2678 529
Size: 35 2079 35 3546
Fragments arranged by size:
3546 2079 35 35
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2499 2643 3142
Size: 54 1425 50 275 18 669 144 499
Cuts at: 3142 4031 5660 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 669 499 275 144 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2807 3625 1353
Size: 1454 818 3423
Fragments arranged by size:
3423 1454 818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4282 4656 4802 387
Size: 1064 138 2693 374 146 1280
Fragments arranged by size:
2693 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2473 2627 3758 4355 5142 5410 1239
Size: 393 841 154 1131 597 787 268 1524
Fragments arranged by size:
1524 1131 841 787 597 393 268 154
EcoO109I rG'GnC_Cy
Cuts at: 2493 2493
Size: 5695
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
EcoRV GAT'ATC
Cuts at: 2874 2874
Size: 5695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2107 2107
Size: 5695
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 4842 1678
Size: 3164 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4336 4368 4410 4442 1373
Size: 32 135 32 2764 32 42 32 2626
Fragments arranged by size:
2764 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4809 4993 1305
Size: 3504 184 2007
Fragments arranged by size:
3504 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2645 10
Size: 54 1425 343 813 3060
Fragments arranged by size:
3060 1425 813 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2639 2828 3653 4012 4171 518
Size: 648 1059 414 189 825 359 159 2042
Fragments arranged by size:
2042 1059 825 648 414 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2891 3111 3788 4936 5181 1606
Size: 1285 220 677 1148 245 2120
Fragments arranged by size:
2120 1285 1148 677 245 220
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5143
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2833 3053 4598 1132
Size: 97 1604 220 1545 2229
Fragments arranged by size:
2229 1604 1545 220 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5695
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5695
PfoI T'CCnGG_A
Cuts at: 2202 2202
Size: 5695
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2493 2493
Size: 5695
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PstI C_TGCA'G
Cuts at: 830 3047 830
Size: 2217 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 3043 4178 4859 5050 826
Size: 219 1998 1135 681 191 1471
Fragments arranged by size:
1998 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 3022 1872
Size: 1150 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2289 2756 2926 3799 4700 4962
Size: 197 926 346 467 170 873 901 262
Cuts at: 4962 820
Size: 1553
Fragments arranged by size:
1553 926 901 873 467 346 262 197
170
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 2512 2962 152
Size: 2360 450 2885
Fragments arranged by size:
2885 2360 450
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 2713 2820 3619 5
Size: 47 2661 107 799 2081
Fragments arranged by size:
2661 2081 799 107 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5695
StyI C'CwwG_G
Cuts at: 513 1065 3134 513
Size: 552 2069 3074
Fragments arranged by size:
3074 2069 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2328 2364 3722 3739 3892 4077 4522
Size: 1255 748 36 1358 17 153 185 445
Cuts at: 4522 325
Size: 1498
Fragments arranged by size:
1498 1358 1255 748 445 185 153 36
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2378 2442 3941
Size: 274 80 33 51 1443 401 64 1499
Cuts at: 3941 5653 96
Size: 1712 138
Fragments arranged by size:
1712 1499 1443 401 274 138 80 64
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2653 2738 2653
Size: 85 5610
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 3020 1870
Size: 1150 4545
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI