Restriction map for
pFN21AE5468
(Circular) (Six-base) MAPSORT of: pFN21AE5468.seq Check: 5006 from: 1 to: 5653
pFN21AE5468 5653 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2568 2974 2568
Size: 406 5247
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2500 2897 2942 3716 5100 5368 1632
Size: 868 397 45 774 1384 268 1917
Fragments arranged by size:
1917 1384 868 774 397 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2647 3396 4968 2647
Size: 749 1572 3332
Fragments arranged by size:
3332 1572 749
ApaLI G'TGCA_C
Cuts at: 2412 3710 4866 2412
Size: 1298 1156 3199
Fragments arranged by size:
3199 1298 1156
ApoI r'AATT_y
Cuts at: 1493 2064 2213 2962 3188 3340 5449 1493
Size: 571 149 749 226 152 2109 1697
Fragments arranged by size:
2109 1697 749 571 226 152 149
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BamHI G'GATC_C
Cuts at: 2930 2983 3350 2930
Size: 53 367 5233
Fragments arranged by size:
5233 367 53
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2568 2974 4429 618
Size: 325 217 627 781 406 1455 1842
Fragments arranged by size:
1842 1455 781 627 406 325 217
BanII G_rGCy'C
Cuts at: 1391 2972 1391
Size: 1581 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 2448 1548
Size: 900 4753
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4264 136
Size: 107 122 71 631 605 2592 1525
Fragments arranged by size:
2592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2416 3714 4870 1790
Size: 626 1298 1156 2573
Fragments arranged by size:
2573 1298 1156 626
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2069 3384 4342
Size: 416 53 83 399 24 652 1315 958
Cuts at: 4342 442
Size: 1753
Fragments arranged by size:
1753 1315 958 652 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2650 4313 1239
Size: 1411 1663 2579
Fragments arranged by size:
2579 1663 1411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2185 2354 3778 4643 4941 2185
Size: 169 1424 865 298 2897
Fragments arranged by size:
2897 1424 865 298 169
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2901 4086 4484 4758 4905 5316 2901
Size: 1185 398 274 147 411 3238
Fragments arranged by size:
3238 1185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2542 1186
Size: 1356 4297
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2416 3714 3799 4870 2416
Size: 1298 85 1071 3199
Fragments arranged by size:
3199 1298 1071 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2416 2972 3714 3799 4870 1391
Size: 399 626 556 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 626 556 399 85
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2580 3008 844
Size: 1736 428 3489
Fragments arranged by size:
3489 1736 428
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2647 794
Size: 1853 3800
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 2555 2555
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2671 2930 2983 3350 3735 3752 5204 5290
Size: 699 259 53 367 385 17 1452 86
Cuts at: 5290 5302 5648 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 699 385 367 346 259 86
53 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2414 3169 3963 3983 2414
Size: 755 794 20 4084
Fragments arranged by size:
4084 794 755 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2293 2328 529
Size: 35 1729 35 3854
Fragments arranged by size:
3854 1729 35 35
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2839 3100 3989
Size: 54 1425 50 275 18 1009 261 889
Cuts at: 3989 5618 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1009 889 275 261 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2519 2735 3583 1353
Size: 1166 216 848 3423
Fragments arranged by size:
3423 1166 848 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2500 2650 2897 2942 3716 4313 5100
Size: 393 868 150 247 45 774 597 787
Cuts at: 5100 5368 1239
Size: 268 1524
Fragments arranged by size:
1524 868 787 774 597 393 268 247
150 45
EcoO109I rG'GnC_Cy
Cuts at: 2939 2939
Size: 5653
EcoRI G'AATT_C
Cuts at: 2064 2213 2962 3340 2064
Size: 149 749 378 4377
Fragments arranged by size:
4377 749 378 149
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373
Size: 32 135 32 2722 32 42 32 2626
Fragments arranged by size:
2722 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2572 2978 2572
Size: 406 5247
MfeI C'AATT_G
Cuts at: 2074 3252 2074
Size: 1178 4475
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2699 4767 4951 1305
Size: 1394 2068 184 2007
Fragments arranged by size:
2068 2007 1394 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2841 10
Size: 54 1425 343 1009 2822
Fragments arranged by size:
2822 1425 1009 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3611 3970 4129 518
Size: 648 2445 359 159 2042
Fragments arranged by size:
2445 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2106 3069 3746 4894 5139 1606
Size: 500 963 677 1148 245 2120
Fragments arranged by size:
2120 1148 963 677 500 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2623 1348
Size: 1275 4378
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PfoI T'CCnGG_A
Cuts at: 2267 2267
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2012 2044 3722 3754 5235 5267 2012
Size: 32 1678 32 1481 32 2398
Fragments arranged by size:
2398 1678 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2939 2939
Size: 5653
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2106 2106
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SanDI GG'GwC_CC
Cuts at: 2939 2939
Size: 5653
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 3001 4136 4817 5008 826
Size: 219 1956 1135 681 191 1471
Fragments arranged by size:
1956 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2164 2369 3757 4658 4920 820
Size: 197 926 221 205 1388 901 262 1553
Fragments arranged by size:
1553 1388 926 901 262 221 205 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2934 3092 513
Size: 552 1869 158 3074
Fragments arranged by size:
3074 1869 552 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2399 3680 3697 3850 4035 4480 325
Size: 1255 819 1281 17 153 185 445 1498
Fragments arranged by size:
1498 1281 1255 819 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3899 5611 96
Size: 274 80 33 51 1443 1922 1712 138
Fragments arranged by size:
1922 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI