Restriction map for
pFN21AE5466
(Circular) (Six-base) MAPSORT of: pFN21AE5466.seq Check: 387 from: 1 to: 5635
pFN21AE5466 5635 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2732 2732
Size: 5635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2386 2978 1937
Size: 449 592 4594
Fragments arranged by size:
4594 592 449
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2479 2587 2718 3698 5082 5350 1632
Size: 847 108 131 980 1384 268 1917
Fragments arranged by size:
1917 1384 980 847 268 131 108
AflII C'TTAA_G
Cuts at: 820 1017 2708 820
Size: 197 1691 3747
Fragments arranged by size:
3747 1691 197
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 3378 4950 3378
Size: 1572 4063
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2624 2944 3170 3322 5431 1493
Size: 1131 320 226 152 2109 1697
Fragments arranged by size:
2109 1697 1131 320 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2960 1870
Size: 73 1017 4545
Fragments arranged by size:
4545 1017 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2956 4411 618
Size: 325 217 627 1169 1455 1842
Fragments arranged by size:
1842 1455 1169 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2447 2954 1391
Size: 1056 507 4072
Fragments arranged by size:
4072 1056 507
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 928
Size: 971 187 4477
Fragments arranged by size:
4477 971 187
BbvCI CC'TCA_GC
Cuts at: 2227 2227
Size: 5635
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2592 5630 2592
Size: 3038 2597
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 3696 4852 1790
Size: 1906 1156 2573
Fragments arranged by size:
2573 1906 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2296 3366 4324
Size: 416 53 83 399 24 879 1070 958
Cuts at: 4324 442
Size: 1753
Fragments arranged by size:
1753 1070 958 879 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2272 4295 1239
Size: 1033 2023 2579
Fragments arranged by size:
2579 2023 1033
Bpu10I CC'TnA_GC
Cuts at: 2227 2227
Size: 5635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2916 3760 4625 4923 2916
Size: 844 865 298 3628
Fragments arranged by size:
3628 865 844 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2736 4298 882
Size: 617 1237 1562 2219
Fragments arranged by size:
2219 1562 1237 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 2075 5629 2075
Size: 3554 2081
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3824 4459 275
Size: 53 83 186 936 1909 382 635 1451
Fragments arranged by size:
1909 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5410
Fragments arranged by size:
5410 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2215 1186
Size: 1029 4606
BseYI C'CCAG_C
Cuts at: 2440 4838 5334 2440
Size: 2398 496 2741
Fragments arranged by size:
2741 2398 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5062
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3696 3781 4852 3696
Size: 85 1071 4479
Fragments arranged by size:
4479 1071 85
BsmI GAATG_Cn'
Cuts at: 2691 3146 3239 2691
Size: 455 93 5087
Fragments arranged by size:
5087 455 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2447 2954 3696 3781 4852 1391
Size: 399 657 507 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 507 399 85
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2410 2732 2990 844
Size: 1566 322 258 3489
Fragments arranged by size:
3489 1566 322 258
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2232 4130 4304 65
Size: 1183 984 1898 174 1396
Fragments arranged by size:
1898 1396 1183 984 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4279 4466 1700
Size: 110 2469 187 2869
Fragments arranged by size:
2869 2469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2248
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2592 2965 3332 3717 3734 5186 5272 5284
Size: 620 373 367 385 17 1452 86 12
Cuts at: 5284 5630 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 620 385 373 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2783 2783
Size: 5635
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3793
Fragments arranged by size:
3793 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 3151 3945 3965 3151
Size: 794 20 4821
Fragments arranged by size:
4821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2287 2322 2538 2573 2801 2836 529
Size: 35 1723 35 216 35 228 35 3328
Fragments arranged by size:
3328 1723 228 216 35 35 35 35
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3082 3971
Size: 54 1425 50 275 18 546 706 889
Cuts at: 3971 5600 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 706 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 2759 3565 1353
Size: 1253 153 806 3423
Fragments arranged by size:
3423 1253 806 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2479 2587 2718 3698 4295 5082
Size: 393 640 207 108 131 980 597 787
Cuts at: 5082 5350 1239
Size: 268 1524
Fragments arranged by size:
1524 980 787 640 597 393 268 207
131 108
EcoO109I rG'GnC_Cy
Cuts at: 2062 2062
Size: 5635
EcoRI G'AATT_C
Cuts at: 2624 2944 3322 2624
Size: 320 378 4937
Fragments arranged by size:
4937 378 320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2723 2723
Size: 5635
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 4782 1678
Size: 3104 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4276 4308 4350 4382 1373
Size: 32 135 32 2704 32 42 32 2626
Fragments arranged by size:
2704 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 2387 2979 3225 669
Size: 988 281 274 175 592 246 3079
Fragments arranged by size:
3079 988 592 281 274 246 175
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4749 4933 1305
Size: 3444 184 2007
Fragments arranged by size:
3444 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3267
Fragments arranged by size:
3267 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2408 2684 2768 2774 2852 3593
Size: 648 1011 231 276 84 6 78 741
Cuts at: 3593 3952 4111 518
Size: 359 159 2042
Fragments arranged by size:
2042 1011 741 648 359 276 231 159
84 78 6
MspA1I CmG'CkG
Cuts at: 1606 2292 2696 3051 3728 4876 5121 1606
Size: 686 404 355 677 1148 245 2120
Fragments arranged by size:
2120 1148 686 677 404 355 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3793
Fragments arranged by size:
3793 939 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2993 4538 1132
Size: 97 1764 1545 2229
Fragments arranged by size:
2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2062
Size: 5635
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2292 2696 2292
Size: 404 5231
SalI G'TCGA_C
Cuts at: 1936 2385 2977 1936
Size: 449 592 4594
Fragments arranged by size:
4594 592 449
SapI GCTCTTCn'nnn_
Cuts at: 2606 2606
Size: 5635
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SexAI A'CCwGG_T
Cuts at: 2109 2109
Size: 5635
SfcI C'TryA_G
Cuts at: 826 1045 2398 2872 2983 4118 4799 4990 826
Size: 219 1353 474 111 1135 681 191 1471
Fragments arranged by size:
1471 1353 1135 681 474 219 191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2708 2931 3739 4640 4902 820
Size: 197 926 765 223 808 901 262 1553
Fragments arranged by size:
1553 926 901 808 765 262 223 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 2131 3559 5
Size: 47 2079 1428 2081
Fragments arranged by size:
2081 2079 1428 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 3074 513
Size: 552 939 351 719 3074
Fragments arranged by size:
3074 939 719 552 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 2191 2315 3662 3679 3832 4017
Size: 1255 492 119 124 1347 17 153 185
Cuts at: 4017 4462 325
Size: 445 1498
Fragments arranged by size:
1498 1347 1255 492 445 185 153 124
119 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3881 5593 96
Size: 274 80 33 51 1443 1904 1712 138
Fragments arranged by size:
1904 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 5595 5627 2321
Size: 32 3242 32 2329
Fragments arranged by size:
3242 2329 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 2689 3764 2689
Size: 1075 4560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI