Restriction map for
pFN21AE5452
(Circular) (Six-base) MAPSORT of: pFN21AE5452.seq Check: 1623 from: 1 to: 5758
pFN21AE5452 5758 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2666 2904 3821 5205 5473 1632
Size: 1034 238 917 1384 268 1917
Fragments arranged by size:
1917 1384 1034 917 268 238
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 2265 4589 2265
Size: 2324 3434
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2487 1184
Size: 1303 4455
AlwNI CAG_nnn'CTG
Cuts at: 2125 2754 3501 5073 2125
Size: 629 747 1572 2810
Fragments arranged by size:
2810 1572 747 629
ApaI G_GGCC'C
Cuts at: 2034 2034
Size: 5758
ApaLI G'TGCA_C
Cuts at: 2177 3815 4971 2177
Size: 1638 1156 2964
Fragments arranged by size:
2964 1638 1156
ApoI r'AATT_y
Cuts at: 1493 3067 3293 3445 5554 1493
Size: 1574 226 152 2109 1697
Fragments arranged by size:
2109 1697 1574 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2439 4312 160
Size: 872 1407 1873 1606
Fragments arranged by size:
1873 1606 1407 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2218 3083 1870
Size: 73 275 865 4545
Fragments arranged by size:
4545 865 275 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2949 3079 4534 618
Size: 325 217 627 1162 130 1455 1842
Fragments arranged by size:
1842 1455 1162 627 325 217 130
BanII G_rGCy'C
Cuts at: 1391 2034 3077 1391
Size: 643 1043 4072
Fragments arranged by size:
4072 1043 643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2239 928
Size: 971 340 4447
Fragments arranged by size:
4447 971 340
BbvCI CC'TCA_GC
Cuts at: 2630 2630
Size: 5758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BfrBI ATG'CAT
Cuts at: 2406 2406
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 4369 136
Size: 107 122 71 631 605 380 2317 1525
Fragments arranged by size:
2317 1525 631 605 380 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2181 3819 4975 1790
Size: 244 147 1638 1156 2573
Fragments arranged by size:
2573 1638 1156 244 147
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2212 2276 2382 2470 4418 1239
Size: 973 64 106 88 1948 2579
Fragments arranged by size:
2579 1948 973 106 88 64
Bpu10I CC'TnA_GC
Cuts at: 2630 2630
Size: 5758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4421 882
Size: 617 2922 2219
Fragments arranged by size:
2922 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2107 3549 493
Size: 711 903 1442 2702
Fragments arranged by size:
2702 1442 903 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2206 2357 2849 3565
Size: 53 83 186 936 673 151 492 716
Cuts at: 3565 3947 4582 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 716 673 635 492 382 186
151 83 53
BsaWI w'CCGG_w
Cuts at: 2265 4191 4589 4863 5010 5421 2265
Size: 1926 398 274 147 411 2602
Fragments arranged by size:
2602 1926 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 2903 2933 1178
Size: 30 165 30 1149 30 321 30 4003
Fragments arranged by size:
4003 1149 321 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5758
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2675 2754 1163
Size: 573 939 79 4167
Fragments arranged by size:
4167 939 573 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2081 2181 3819 3904 4975 2081
Size: 100 1638 85 1071 2864
Fragments arranged by size:
2864 1638 1071 100 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2081 2181 3077 3819 3904 4975
Size: 399 244 47 100 896 742 85 1071
Cuts at: 4975 1391
Size: 2174
Fragments arranged by size:
2174 1071 896 742 399 244 100 85
47
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3113 844
Size: 2269 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2052 3645 1394
Size: 429 229 1593 3507
Fragments arranged by size:
3507 1593 429 229
BsrDI GCAATG_nn'
Cuts at: 65 1248 2406 2469 2617 2877 4253 4427 65
Size: 1183 1158 63 148 260 1376 174 1396
Fragments arranged by size:
1396 1376 1183 1158 260 174 148 63
BsrFI r'CCGG_y
Cuts at: 1700 1810 2265 4402 4589 1700
Size: 110 455 2137 187 2869
Fragments arranged by size:
2869 2137 455 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 2497 1184
Size: 1313 4445
BstYI r'GATC_y
Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753
Size: 1116 367 385 17 1452 86 12 346
Cuts at: 5753 1972
Size: 1977
Fragments arranged by size:
1977 1452 1116 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 2072 2706 513
Size: 552 939 21 47 634 3565
Fragments arranged by size:
3565 939 634 552 47 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 3274 4068 4088 3274
Size: 794 20 4944
Fragments arranged by size:
4944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DraIII CAC_nnn'GTG
Cuts at: 2185 2185
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2270 4765 809
Size: 507 954 2495 1802
Fragments arranged by size:
2495 1802 954 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 3205 4094
Size: 54 1425 50 275 18 408 967 889
Cuts at: 4094 5723 8
Size: 1629 43
Fragments arranged by size:
1629 1425 967 889 408 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2532 3688 1353
Size: 1179 1156 3423
Fragments arranged by size:
3423 1179 1156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2212 2276 2382 2470 2666 2904 3821
Size: 393 580 64 106 88 196 238 917
Cuts at: 3821 4418 5205 5473 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 917 787 597 580 393 268 238
196 106 88 64
EcoO109I rG'GnC_Cy
Cuts at: 2030 2031 2223 2030
Size: 1 192 5565
Fragments arranged by size:
5565 192 1
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 2172 4905 1678
Size: 494 2733 2531
Fragments arranged by size:
2733 2531 494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4399 4431 4473 4505 1373
Size: 32 135 32 2827 32 42 32 2626
Fragments arranged by size:
2827 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 3102 3348 669
Size: 988 281 622 542 246 3079
Fragments arranged by size:
3079 988 622 542 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2703 2954 4872 5056 1305
Size: 1398 251 1918 184 2007
Fragments arranged by size:
2007 1918 1398 251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3936
Fragments arranged by size:
3936 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3716 4075 4234 518
Size: 648 2550 359 159 2042
Fragments arranged by size:
2550 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2091 3174 3851 4999 5244 1606
Size: 412 73 1083 677 1148 245 2120
Fragments arranged by size:
2120 1148 1083 677 412 245 73
NcoI C'CATG_G
Cuts at: 513 1065 2004 2025 2706 513
Size: 552 939 21 681 3565
Fragments arranged by size:
3565 939 681 552 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NsiI A_TGCA'T
Cuts at: 2408 2408
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PfoI T'CCnGG_A
Cuts at: 2570 2570
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2223 2223
Size: 5758
PshAI GACnn'nnGTC
Cuts at: 1613 2205 1613
Size: 592 5166
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2030 2030
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2023 3110 830
Size: 1193 1087 3478
Fragments arranged by size:
3478 1193 1087
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SapI GCTCTTCn'nnn_
Cuts at: 2532 2532
Size: 5758
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 2717 4006 5718 2717
Size: 1289 1712 2757
Fragments arranged by size:
2757 1712 1289
SfcI C'TryA_G
Cuts at: 826 1045 2019 2475 3106 4241 4922 5113 826
Size: 219 974 456 631 1135 681 191 1471
Fragments arranged by size:
1471 1135 974 681 631 456 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2052 2052
Size: 5758
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3862 4763 5025 820
Size: 197 926 1919 901 262 1553
Fragments arranged by size:
1919 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 2962 1517
Size: 1445 4313
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 2172 2706 3197 513
Size: 552 939 21 147 534 491 3074
Fragments arranged by size:
3074 939 552 534 491 147 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3785 3802 3955 4140 4585 325
Size: 1255 2205 17 153 185 445 1498
Fragments arranged by size:
2205 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2715 4004 5716
Size: 274 80 33 51 1443 738 1289 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1712 1443 1289 738 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2903 2935 5718 5750 2903
Size: 32 2783 32 2911
Fragments arranged by size:
2911 2783 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfoI SrfI SwaI XcmI