Restriction map for
pFN21AE5446
(Circular) (Six-base) MAPSORT of: pFN21AE5446.seq Check: 9927 from: 1 to: 5671
pFN21AE5446 5671 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2189 2189
Size: 5671
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2560 2584 3734 5118 5386 1632
Size: 928 24 1150 1384 268 1917
Fragments arranged by size:
1917 1384 1150 928 268 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2459 1184
Size: 1275 4396
AlwNI CAG_nnn'CTG
Cuts at: 2031 2280 2503 3414 4986 2031
Size: 249 223 911 1572 2716
Fragments arranged by size:
2716 1572 911 249 223
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2897 2980 3206 3358 5467 1493
Size: 1404 83 226 152 2109 1697
Fragments arranged by size:
2109 1697 1404 226 152 83
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 2996 1870
Size: 73 942 111 4545
Fragments arranged by size:
4545 942 111 73
BamHI G'GATC_C
Cuts at: 2762 3001 3368 2762
Size: 239 367 5065
Fragments arranged by size:
5065 367 239
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2347 2992 4447 618
Size: 325 217 627 300 260 645 1455 1842
Fragments arranged by size:
1842 1455 645 627 325 300 260 217
BanII G_rGCy'C
Cuts at: 1391 2022 2700 2783 2941 2990 1391
Size: 631 678 83 158 49 4072
Fragments arranged by size:
4072 678 631 158 83 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2365 2374 2566 928
Size: 971 466 9 192 4033
Fragments arranged by size:
4033 971 466 192 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3862 3896 1903
Size: 34 50 34 1841 34 3678
Fragments arranged by size:
3678 1841 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BfrBI ATG'CAT
Cuts at: 2812 2812
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2456 4282 136
Size: 107 122 71 631 605 784 1826 1525
Fragments arranged by size:
1826 1525 784 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 2438 2452 3732 4888 1790
Size: 648 14 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 648 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2004 2290 3402
Size: 416 53 83 399 24 587 286 1112
Cuts at: 3402 4360 442
Size: 958 1753
Fragments arranged by size:
1753 1112 958 587 416 399 286 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2614 2802 4331 1239
Size: 950 425 188 1529 2579
Fragments arranged by size:
2579 1529 950 425 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2694 3796 4661 4959 2694
Size: 1102 865 298 3406
Fragments arranged by size:
3406 1102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 1178
Size: 30 165 30 711 30 4705
Fragments arranged by size:
4705 711 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5671
BseYI C'CCAG_C
Cuts at: 2015 2031 2273 2351 2950 4874 5370 2015
Size: 16 242 78 599 1924 496 2316
Fragments arranged by size:
2316 1924 599 496 242 78 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2946 1163
Size: 573 498 712 3888
Fragments arranged by size:
3888 712 573 498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2783 3732 3817 4888 2783
Size: 949 85 1071 3566
Fragments arranged by size:
3566 1071 949 85
BsmI GAATG_Cn'
Cuts at: 2812 2814 3182 3275 2812
Size: 2 368 93 5208
Fragments arranged by size:
5208 368 93 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2438 2452 2700 2783 2941 2990
Size: 399 232 416 14 248 83 158 49
Cuts at: 2990 3732 3817 4888 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 416 399 248 232 158
85 83 49 14
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2189 2206 2354 3026 844
Size: 1241 104 17 148 672 3489
Fragments arranged by size:
3489 1241 672 148 104 17
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4315 4502 1700
Size: 110 2505 187 2869
Fragments arranged by size:
2869 2505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2212 794
Size: 1418 4253
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2754 2762 3001 3368 3753 3770 5222 5308
Size: 782 8 239 367 385 17 1452 86
Cuts at: 5308 5320 5666 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 782 385 367 346 239 86
17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2199 2568 2731 513
Size: 552 1134 369 163 3453
Fragments arranged by size:
3453 1134 552 369 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 3187 3981 4001 3187
Size: 794 20 4857
Fragments arranged by size:
4857 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2457 2665
Size: 54 1425 50 275 18 208 419 208
Cuts at: 2665 3118 4007 5636 8
Size: 453 889 1629 43
Fragments arranged by size:
1629 1425 889 453 419 275 208 208
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3601 1353
Size: 2248 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2135 4258 4632 4778 387
Size: 1064 138 546 2123 374 146 1280
Fragments arranged by size:
2123 1280 1064 546 374 146 138
EcoICRI GAG'CTC
Cuts at: 2781 2781
Size: 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2560 2584 2614 2802 3734 4331
Size: 393 557 371 24 30 188 932 597
Cuts at: 4331 5118 5386 1239
Size: 787 268 1524
Fragments arranged by size:
1524 932 787 597 557 393 371 268
188 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2802 2802
Size: 5671
EcoO109I rG'GnC_Cy
Cuts at: 2889 2889
Size: 5671
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 5671
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 2494 4818 1678
Size: 816 2324 2531
Fragments arranged by size:
2531 2324 816
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 4312 4344 4386
Size: 32 135 32 542 32 2166 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 2166 542 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 2949 4785 4969 1305
Size: 825 819 1836 184 2007
Fragments arranged by size:
2007 1836 825 819 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2667 10
Size: 54 1425 343 835 3014
Fragments arranged by size:
3014 1425 835 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3629 3988 4147 518
Size: 648 2463 359 159 2042
Fragments arranged by size:
2463 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2104 3087 3764 4912 5157 1606
Size: 422 76 983 677 1148 245 2120
Fragments arranged by size:
2120 1148 983 677 422 245 76
NcoI C'CATG_G
Cuts at: 513 1065 2199 2568 2731 513
Size: 552 1134 369 163 3453
Fragments arranged by size:
3453 1134 552 369 163
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NsiI A_TGCA'T
Cuts at: 2814 2814
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 3029 4574 1132
Size: 97 1800 1545 2229
Fragments arranged by size:
2229 1800 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2536 1348
Size: 1188 4483
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2280 1108
Size: 1172 4499
PfoI T'CCnGG_A
Cuts at: 2166 2166
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2546 1613
Size: 933 4738
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 2027 3023 830
Size: 1197 996 3478
Fragments arranged by size:
3478 1197 996
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2028 2104 2028
Size: 76 5595
SacI G_AGCT'C
Cuts at: 2783 2783
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2023 3019 4154 4835 5026 826
Size: 219 978 996 1135 681 191 1471
Fragments arranged by size:
1471 1135 996 978 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2887 2998 1872
Size: 1015 111 4545
Fragments arranged by size:
4545 1015 111
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2709 3775 4676 4938 820
Size: 197 926 766 1066 901 262 1553
Fragments arranged by size:
1553 1066 926 901 766 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2199 2568 2731 3110 513
Size: 552 1134 369 163 379 3074
Fragments arranged by size:
3074 1134 552 379 369 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2224 2365 2581 3698 3715 3868 4053
Size: 1255 644 141 216 1117 17 153 185
Cuts at: 4053 4498 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1117 644 445 216 185 153
141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2729 2729
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2885 2996 1870
Size: 1015 111 4545
Fragments arranged by size:
4545 1015 111
XmnI GAAnn'nnTTC
Cuts at: 2812 3800 2812
Size: 988 4683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI