Restriction map for
pFN21AE5444
(Circular) (Six-base) MAPSORT of: pFN21AE5444.seq Check: 3555 from: 1 to: 5641
pFN21AE5444 5641 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2389 2984 1937
Size: 452 595 4594
Fragments arranged by size:
4594 595 452
Acc65I G'GTAC_C
Cuts at: 2503 2962 2503
Size: 459 5182
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2693 3704 5088 5356 1632
Size: 1061 1011 1384 268 1917
Fragments arranged by size:
1917 1384 1061 1011 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2726 4540 5517 1203
Size: 1523 1814 977 1327
Fragments arranged by size:
1814 1523 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 2251 3384 4956 2251
Size: 1133 1572 2936
Fragments arranged by size:
2936 1572 1133
ApaI G_GGCC'C
Cuts at: 2270 2270
Size: 5641
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2803 2950 3176 3328 5437 1493
Size: 1310 147 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 226 152 147
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2673 2966 1870
Size: 73 730 293 4545
Fragments arranged by size:
4545 730 293 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2503 2663 2962 4417 618
Size: 325 217 627 716 160 299 1455 1842
Fragments arranged by size:
1842 1455 716 627 325 299 217 160
BanII G_rGCy'C
Cuts at: 1391 2270 2618 2737 2960 1391
Size: 879 348 119 223 4072
Fragments arranged by size:
4072 879 348 223 119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2040 928
Size: 971 141 4529
Fragments arranged by size:
4529 971 141
BbvCI CC'TCA_GC
Cuts at: 2070 2070
Size: 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 2812 1548
Size: 1264 4377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2270 3702 4858 1790
Size: 480 1432 1156 2573
Fragments arranged by size:
2573 1432 1156 480
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2336 2712 3372
Size: 416 53 83 399 24 919 376 660
Cuts at: 3372 4330 442
Size: 958 1753
Fragments arranged by size:
1753 958 919 660 416 399 376 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2771 4301 1239
Size: 1532 1530 2579
Fragments arranged by size:
2579 1532 1530
Bpu10I CC'TnA_GC
Cuts at: 2070 2070
Size: 5641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2158 3432 493
Size: 711 954 1274 2702
Fragments arranged by size:
2702 1274 954 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2147 2177 1178
Size: 30 165 30 744 30 4642
Fragments arranged by size:
4642 744 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5641
BseYI C'CCAG_C
Cuts at: 4844 5340 4844
Size: 496 5145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2618 3702 3787 4858 2618
Size: 1084 85 1071 3401
Fragments arranged by size:
3401 1084 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2270 2618 2737 2960 3702 3787 4858
Size: 399 480 348 119 223 742 85 1071
Cuts at: 4858 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 480 399 348 223 119
85
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2610 3530 5260 2610
Size: 920 1730 2991
Fragments arranged by size:
2991 1730 920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2996 844
Size: 2152 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2667 2826 4136 4310 65
Size: 1183 1419 159 1310 174 1396
Fragments arranged by size:
1419 1396 1310 1183 174 159
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2215 2971 3338 3723 3740 5192 5278 5290
Size: 243 756 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 756 385 367 346 243 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5089
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2504 487
Size: 683 56 1278 3624
Fragments arranged by size:
3624 1278 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3088 3977 5606
Size: 54 1425 50 275 18 1258 889 1629
Cuts at: 5606 8
Size: 43
Fragments arranged by size:
1629 1425 1258 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2906 3571 1353
Size: 1553 665 3423
Fragments arranged by size:
3423 1553 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2616 2616
Size: 5641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2693 2771 3704 4301 5088 5356 1239
Size: 393 1061 78 933 597 787 268 1524
Fragments arranged by size:
1524 1061 933 787 597 393 268 78
EcoO109I rG'GnC_Cy
Cuts at: 2266 2266
Size: 5641
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2848 2880 4282 4314 4356
Size: 32 135 32 1276 32 1402 32 42
Cuts at: 4356 4388 1373
Size: 32 2626
Fragments arranged by size:
2626 1402 1276 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2578 2985 3231 669
Size: 988 281 640 407 246 3079
Fragments arranged by size:
3079 988 640 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5641
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2507 2966 2507
Size: 459 5182
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2589 2609 2777 3599 3958 4117
Size: 648 1014 409 20 168 822 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 1014 822 648 409 359 168 159
20
MspA1I CmG'CkG
Cuts at: 1606 2074 3057 3734 4882 5127 1606
Size: 468 983 677 1148 245 2120
Fragments arranged by size:
2120 1148 983 677 468 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5089
NdeI CA'TA_TG
Cuts at: 387 2365 387
Size: 1978 3663
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2692 2730 2782 2897 2999 4544 1132
Size: 97 1463 38 52 115 102 1545 2229
Fragments arranged by size:
2229 1545 1463 115 102 97 52 38
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 2726 4540 2726
Size: 1814 3827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 2743 1108
Size: 1329 306 4006
Fragments arranged by size:
4006 1329 306
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2266 2266
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2074 2074
Size: 5641
SacI G_AGCT'C
Cuts at: 2618 2618
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2989 4124 4805 4996 826
Size: 219 1944 1135 681 191 1471
Fragments arranged by size:
1944 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2675 2968 1872
Size: 803 293 4545
Fragments arranged by size:
4545 803 293
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2692 2999 1132
Size: 1560 307 3774
Fragments arranged by size:
3774 1560 307
SrfI GCCC'GGGC
Cuts at: 2675 2675
Size: 5641
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 3080 513
Size: 552 2015 3074
Fragments arranged by size:
3074 2015 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2597 3668 3685 3838 4023 4468 325
Size: 1255 1017 1071 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1071 1017 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2680 3887
Size: 274 80 33 51 1443 207 496 1207
Cuts at: 3887 5599 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1207 496 274 207 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2673 2966 1870
Size: 803 293 4545
Fragments arranged by size:
4545 803 293
XmnI GAAnn'nnTTC
Cuts at: 2082 3770 2082
Size: 1688 3953
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI
XcmI