Restriction map for
pFN21AE5443
(Circular) (Six-base) MAPSORT of: pFN21AE5443.seq Check: 1395 from: 1 to: 5644
pFN21AE5443 5644 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2483 2704 2987 1937
Size: 546 221 283 4594
Fragments arranged by size:
4594 546 283 221
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2718 3707 5091 5359 1632
Size: 1086 989 1384 268 1917
Fragments arranged by size:
1917 1384 1086 989 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2228 2723 4543 5520 1203
Size: 1025 495 1820 977 1327
Fragments arranged by size:
1820 1327 1025 977 495
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2713 2745 2713
Size: 32 5612
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2254 2953 3179 3331 5440 1493
Size: 761 699 226 152 2109 1697
Fragments arranged by size:
2109 1697 761 699 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 4420 618
Size: 325 217 627 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2619 2963 1391
Size: 1228 344 4072
Fragments arranged by size:
4072 1228 344
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 2920 928
Size: 971 187 834 3652
Fragments arranged by size:
3652 971 834 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3835 3869 1903
Size: 34 1898 34 3678
Fragments arranged by size:
3678 1898 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2679 3536 4746 2679
Size: 857 1210 3577
Fragments arranged by size:
3577 1210 857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2327 4255 136
Size: 107 122 71 631 605 655 1928 1525
Fragments arranged by size:
1928 1525 655 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 3705 4861 1790
Size: 1915 1156 2573
Fragments arranged by size:
2573 1915 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2414 2480 4304 1239
Size: 854 321 66 1824 2579
Fragments arranged by size:
2579 1824 854 321 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 3769 4634 4932 2543
Size: 1226 865 298 3255
Fragments arranged by size:
3255 1226 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4077 4475 4749 4896 5307 4077
Size: 398 274 147 411 4414
Fragments arranged by size:
4414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2412 2442 1178
Size: 30 165 30 1009 30 4380
Fragments arranged by size:
4380 1009 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5644
BseYI C'CCAG_C
Cuts at: 2047 2301 2798 4847 5343 2047
Size: 254 497 2049 496 2348
Fragments arranged by size:
2348 2049 497 496 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2535 1163
Size: 573 799 4272
Fragments arranged by size:
4272 799 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2619 3705 3790 4861 2619
Size: 1086 85 1071 3402
Fragments arranged by size:
3402 1086 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
BsmBI CGTCTCn'nnnn_
Cuts at: 2776 2776
Size: 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2619 2963 3705 3790 4861 1391
Size: 399 829 344 742 85 1071 2174
Fragments arranged by size:
2174 1071 829 742 399 344 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2790 2999 844
Size: 1946 209 3489
Fragments arranged by size:
3489 1946 209
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 1394
Size: 429 1708 3507
Fragments arranged by size:
3507 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2844 4139 4313 65
Size: 1183 1596 1295 174 1396
Fragments arranged by size:
1596 1396 1295 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 2231 96
Size: 2135 3509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2212 2974 3341 3726 3743 5195 5281 5293
Size: 240 762 367 385 17 1452 86 12
Cuts at: 5293 5639 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 762 385 367 346 240 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5092
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 3160 3954 3974 2552
Size: 608 794 20 4222
Fragments arranged by size:
4222 794 608 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2908 2948 2908
Size: 40 5604
DraIII CAC_nnn'GTG
Cuts at: 2510 2510
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 3091 3980
Size: 54 1425 50 275 18 519 742 889
Cuts at: 3980 5609 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 742 519 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2624 2837 3574 1353
Size: 1271 213 737 3423
Fragments arranged by size:
3423 1271 737 213
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2617 2617
Size: 5644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2414 2480 2718 3707 4304 5091
Size: 393 461 321 66 238 989 597 787
Cuts at: 5091 5359 1239
Size: 268 1524
Fragments arranged by size:
1524 989 787 597 461 393 321 268
238 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2365 2365
Size: 5644
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 5644
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 4791 1678
Size: 3113 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2412 2444 2812 2844 4285
Size: 32 135 32 840 32 368 32 1441
Cuts at: 4285 4317 4359 4391 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1441 840 368 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2705 2988 3234 669
Size: 988 281 767 283 246 3079
Fragments arranged by size:
3079 988 767 283 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5644
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3602 3961 4120 518
Size: 648 2436 359 159 2042
Fragments arranged by size:
2436 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2441 2500 3060 3737 4885 5130 1606
Size: 835 59 560 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 677 560 245 59
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5092
NdeI CA'TA_TG
Cuts at: 387 2656 387
Size: 2269 3375
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NspI r_CATG'y
Cuts at: 1132 1229 2232 2727 3002 4547 1132
Size: 97 1003 495 275 1545 2229
Fragments arranged by size:
2229 1545 1003 495 275 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 2228 2723 4543 2228
Size: 495 1820 3329
Fragments arranged by size:
3329 1820 495
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4742
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2996 830
Size: 2166 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2441 2500 2441
Size: 59 5585
SacI G_AGCT'C
Cuts at: 2619 2619
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2703 2986 1936
Size: 767 283 4594
Fragments arranged by size:
4594 767 283
SapI GCTCTTCn'nnn_
Cuts at: 2624 2624
Size: 5644
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2992 4127 4808 4999 826
Size: 219 1947 1135 681 191 1471
Fragments arranged by size:
1947 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 3748 4649 4911 820
Size: 197 926 615 1190 901 262 1553
Fragments arranged by size:
1553 1190 926 901 615 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2066 2322 2805 2812 3083 513
Size: 552 1001 256 483 7 271 3074
Fragments arranged by size:
3074 1001 552 483 271 256 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2866 3671 3688 3841 4026 4471 325
Size: 1255 1286 805 17 153 185 445 1498
Fragments arranged by size:
1498 1286 1255 805 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2231 2762 3890
Size: 274 80 33 51 1443 254 531 1128
Cuts at: 3890 5602 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1128 531 274 254 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 3773 2340
Size: 1433 4211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI