Restriction map for
pFN21AE5441
(Circular) (Six-base) MAPSORT of: pFN21AE5441.seq Check: 5839 from: 1 to: 5656
pFN21AE5441 5656 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2081 2826 2999 1937
Size: 144 745 173 4594
Fragments arranged by size:
4594 745 173 144
Acc65I G'GTAC_C
Cuts at: 2977 2977
Size: 5656
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 3719 5103 5371 1632
Size: 986 1101 1384 268 1917
Fragments arranged by size:
1917 1384 1101 986 268
AfeI AGC'GCT
Cuts at: 2450 2450
Size: 5656
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5459
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2143 2253 2587 2596 3399 4971 2143
Size: 110 334 9 803 1572 2828
Fragments arranged by size:
2828 1572 803 334 110 9
ApaI G_GGCC'C
Cuts at: 2738 2914 2738
Size: 176 5480
ApaLI G'TGCA_C
Cuts at: 3713 4869 3713
Size: 1156 4500
ApoI r'AATT_y
Cuts at: 1493 2965 3191 3343 5452 1493
Size: 1472 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2444 2819 2981 1870
Size: 73 501 375 162 4545
Fragments arranged by size:
4545 501 375 162 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2414 2759 2920 2977
Size: 325 217 627 264 363 345 161 57
Cuts at: 2977 4432 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 363 345 325 264 217
161 57
BanII G_rGCy'C
Cuts at: 1391 2388 2738 2914 2936 2975 1391
Size: 997 350 176 22 39 4072
Fragments arranged by size:
4072 997 350 176 39 22
BbeI G_GCGC'C
Cuts at: 2763 2763
Size: 5656
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2783 2817 3847 3881 1903
Size: 34 846 34 1030 34 3678
Fragments arranged by size:
3678 1030 846 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2100 3548 4758 2100
Size: 1448 1210 2998
Fragments arranged by size:
2998 1448 1210
BclI T'GATC_A
Cuts at: 1548 2259 1548
Size: 711 4945
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2918 4267 136
Size: 107 122 71 631 605 1246 1349 1525
Fragments arranged by size:
1525 1349 1246 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5651 5651
Size: 5656
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2738 2914 2923 3717 4873 1790
Size: 948 176 9 794 1156 2573
Fragments arranged by size:
2573 1156 948 794 176 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2037 4316 1239
Size: 798 2279 2579
Fragments arranged by size:
2579 2279 798
Bpu10I CC'TnA_GC
Cuts at: 2898 2898
Size: 5656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3781 4646 4944 3781
Size: 865 298 4493
Fragments arranged by size:
4493 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 2760 3463 3845
Size: 53 83 186 936 545 682 703 382
Cuts at: 3845 4480 275
Size: 635 1451
Fragments arranged by size:
1451 936 703 682 635 545 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5431
Fragments arranged by size:
5431 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5656
BseYI C'CCAG_C
Cuts at: 2026 2119 2136 2253 2320 2335 2541 2787 4859
Size: 93 17 117 67 15 206 246 2072
Cuts at: 4859 5355 2026
Size: 496 2327
Fragments arranged by size:
2327 2072 496 246 206 117 93 67
17 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 2900 1163
Size: 573 706 458 3919
Fragments arranged by size:
3919 706 573 458
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3717 3802 4873 3717
Size: 85 1071 4500
Fragments arranged by size:
4500 1071 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
BsmBI CGTCTCn'nnnn_
Cuts at: 2447 2447
Size: 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2388 2738 2914 2923 2936 2975 3717
Size: 399 598 350 176 9 13 39 742
Cuts at: 3717 3802 4873 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 598 399 350 176 85
39 13 9
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2222 3011 844
Size: 1378 789 3489
Fragments arranged by size:
3489 1378 789
BsrBI CCG'CTC
Cuts at: 1394 1823 2470 2707 2850 3543 1394
Size: 429 647 237 143 693 3507
Fragments arranged by size:
3507 693 647 429 237 143
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 4151 4325 65
Size: 1183 1179 1724 174 1396
Fragments arranged by size:
1724 1396 1183 1179 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2687 2774 4300 4487 1700
Size: 110 877 87 1526 187 2869
Fragments arranged by size:
2869 1526 877 187 110 87
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 2893 2948 1925
Size: 968 55 4633
Fragments arranged by size:
4633 968 55
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2429 2878 794
Size: 1635 449 3572
Fragments arranged by size:
3572 1635 449
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2213 2936 2213
Size: 723 4933
BstYI r'GATC_y
Cuts at: 1972 2532 2986 3353 3738 3755 5207 5293 5305
Size: 560 454 367 385 17 1452 86 12
Cuts at: 5305 5651 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 560 454 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2915 2915
Size: 5656
BtgI C'CryG_G
Cuts at: 513 1065 2004 2887 513
Size: 552 939 883 3282
Fragments arranged by size:
3282 939 883 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 2224 2262 2524 3172 3966 3986 2224
Size: 38 262 648 794 20 3894
Fragments arranged by size:
3894 794 648 262 38 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2960 2960
Size: 5656
DraIII CAC_nnn'GTG
Cuts at: 2395 2395
Size: 5656
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2621 4663 809
Size: 507 1305 2042 1802
Fragments arranged by size:
2042 1802 1305 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2772 3103
Size: 54 1425 50 275 18 270 672 331
Cuts at: 3103 3992 5621 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 672 331 275 270 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3586 1353
Size: 2233 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2618 3719 4316 5103 5371 1239
Size: 393 405 581 1101 597 787 268 1524
Fragments arranged by size:
1524 1101 787 597 581 405 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2217 2325 2473 2660 2734 2735 2910 2911 2217
Size: 108 148 187 74 1 175 1 4962
Fragments arranged by size:
4962 187 175 148 108 74 1 1
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 2230 2844 4162 2230
Size: 614 1318 3724
Fragments arranged by size:
3724 1318 614
HaeII r_GCGC'y
Cuts at: 1678 2024 2452 2763 4803 1678
Size: 346 428 311 2040 2531
Fragments arranged by size:
2531 2040 428 346 311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373
Size: 32 135 32 2725 32 42 32 2626
Fragments arranged by size:
2725 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2082 3000 3246 669
Size: 988 281 144 918 246 3079
Fragments arranged by size:
3079 988 918 281 246 144
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5656
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KasI G'GCGC_C
Cuts at: 2759 2759
Size: 5656
KpnI G_GTAC'C
Cuts at: 2981 2981
Size: 5656
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3834
Fragments arranged by size:
3834 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 3973 4132 518
Size: 648 2448 359 159 2042
Fragments arranged by size:
2448 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2253 2335 2787 2889 3072 3749 4897
Size: 537 110 82 452 102 183 677 1148
Cuts at: 4897 5142 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 537 452 245 183 110
102 82
NaeI GCC'GGC
Cuts at: 2689 2776 2689
Size: 87 5569
NarI GG'CG_CC
Cuts at: 2760 2760
Size: 5656
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NgoMIV G'CCGG_C
Cuts at: 2687 2774 2687
Size: 87 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2680 3014 4559 1132
Size: 97 1451 334 1545 2229
Fragments arranged by size:
2229 1545 1451 334 97
PasI CC'CwG_GG
Cuts at: 1348 2032 1348
Size: 684 4972
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2126 2253 2365 1108
Size: 1018 127 112 4399
Fragments arranged by size:
4399 1018 127 112
PfoI T'CCnGG_A
Cuts at: 2402 2402
Size: 5656
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2333 2365 3725 3757 5238 5270 2333
Size: 32 1360 32 1481 32 2719
Fragments arranged by size:
2719 1481 1360 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2217 2473 2660 2217
Size: 256 187 5213
Fragments arranged by size:
5213 256 187
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PspOMI G'GGCC_C
Cuts at: 2734 2910 2734
Size: 176 5480
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2285 2317 2285
Size: 32 5624
PstI C_TGCA'G
Cuts at: 830 2592 2630 3008 830
Size: 1762 38 378 3478
Fragments arranged by size:
3478 1762 378 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
PvuII CAG'CTG
Cuts at: 2143 2335 2787 2143
Size: 192 452 5012
Fragments arranged by size:
5012 452 192
SacII CC_GC'GG
Cuts at: 2890 2890
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2080 2998 1936
Size: 144 918 4594
Fragments arranged by size:
4594 918 144
SanDI GG'GwC_CC
Cuts at: 2217 2660 2217
Size: 443 5213
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2588 2626 3004 4139 4820 5011 826
Size: 219 1543 38 378 1135 681 191 1471
Fragments arranged by size:
1543 1471 1135 681 378 219 191 38
SfoI GGC'GCC
Cuts at: 2761 2761
Size: 5656
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2821 2983 1872
Size: 949 162 4545
Fragments arranged by size:
4545 949 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 3760 4661 4923 820
Size: 197 926 501 1316 901 262 1553
Fragments arranged by size:
1553 1316 926 901 501 262 197
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 3014 1132
Size: 1882 3774
SspI AAT'ATT
Cuts at: 5 52 3580 5
Size: 47 3528 2081
Fragments arranged by size:
3528 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2004 3095 513
Size: 552 939 1091 3074
Fragments arranged by size:
3074 1091 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2245 2343 2357 2368 2537 2845
Size: 1255 444 221 98 14 11 169 308
Cuts at: 2845 3683 3700 3853 4038 4483 325
Size: 838 17 153 185 445 1498
Fragments arranged by size:
1498 1255 838 445 444 308 221 185
169 153 98 17 14 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 3902 5614
Size: 274 80 33 51 1443 660 1265 1712
Cuts at: 5614 96
Size: 138
Fragments arranged by size:
1712 1443 1265 660 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2398 2430 2666 2698 5616 5648 2398
Size: 32 236 32 2918 32 2406
Fragments arranged by size:
2918 2406 236 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 2444 1943
Size: 501 5155
XmaI C'CCGG_G
Cuts at: 1870 2819 2981 1870
Size: 949 162 4545
Fragments arranged by size:
4545 949 162
XmnI GAAnn'nnTTC
Cuts at: 3785 3785
Size: 5656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI RsrII SacI SapI
SexAI SfiI SrfI SwaI XcmI