Restriction map for
pFN21AE5435
(Circular) (Six-base) MAPSORT of: pFN21AE5435.seq Check: 64 from: 1 to: 5659
pFN21AE5435 5659 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2302 2721 2807 2860 2908 2918 3722 5106
Size: 670 419 86 53 48 10 804 1384
Cuts at: 5106 5374 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 804 670 419 268 86 53
48 10
AfeI AGC'GCT
Cuts at: 2273 2273
Size: 5659
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 2260 4388 2260
Size: 2128 3531
AleI CACnn'nnGTG
Cuts at: 1166 2747 1166
Size: 1581 4078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 2809 3402 4974 2809
Size: 593 1572 3494
Fragments arranged by size:
3494 1572 593
ApaLI G'TGCA_C
Cuts at: 2756 3716 4872 2756
Size: 960 1156 3543
Fragments arranged by size:
3543 1156 960
ApoI r'AATT_y
Cuts at: 1493 2968 3194 3346 5455 1493
Size: 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2980 4435 618
Size: 325 217 627 1193 1455 1842
Fragments arranged by size:
1842 1455 1193 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2417 2978 1391
Size: 1026 561 4072
Fragments arranged by size:
4072 1026 561
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2053 2921 928
Size: 971 154 868 3666
Fragments arranged by size:
3666 971 868 154
BbvCI CC'TCA_GC
Cuts at: 2861 2861
Size: 5659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3850 3884 1903
Size: 34 50 34 1829 34 3678
Fragments arranged by size:
3678 1829 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 2421 2439
Size: 107 122 71 631 605 616 133 18
Cuts at: 2439 4270 136
Size: 1831 1525
Fragments arranged by size:
1831 1525 631 616 605 133 122 107
71 18
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2760 3720 4876 1790
Size: 970 960 1156 2573
Fragments arranged by size:
2573 1156 970 960
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2797 3390 4348
Size: 416 53 83 399 24 1380 593 958
Cuts at: 4348 442
Size: 1753
Fragments arranged by size:
1753 1380 958 593 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2173 4319 1239
Size: 818 116 2146 2579
Fragments arranged by size:
2579 2146 818 116
Bpu10I CC'TnA_GC
Cuts at: 2861 2861
Size: 5659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 3450 493
Size: 711 1170 1076 2702
Fragments arranged by size:
2702 1170 1076 711
BsaBI GATnn'nnATC
Cuts at: 2831 5653 2831
Size: 2822 2837
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2565 2595 1178
Size: 30 165 30 1162 30 4242
Fragments arranged by size:
4242 1162 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2633 2642 1186
Size: 1447 9 4203
Fragments arranged by size:
4203 1447 9
BseYI C'CCAG_C
Cuts at: 2122 2268 2629 4862 5358 2122
Size: 146 361 2233 496 2423
Fragments arranged by size:
2423 2233 496 361 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5086
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3109 3870 4898 1540
Size: 275 186 367 741 761 1028 2301
Fragments arranged by size:
2301 1028 761 741 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 2760 3720 3805 4876 2409
Size: 351 960 85 1071 3192
Fragments arranged by size:
3192 1071 960 351 85
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
BsmBI CGTCTCn'nnnn_
Cuts at: 2212 2568 2212
Size: 356 5303
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 2417 2760 2978 3720 3805 4876
Size: 399 619 8 343 218 742 85 1071
Cuts at: 4876 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 619 399 343 218 85
8
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 2409 3548 5278 2409
Size: 1139 1730 2790
Fragments arranged by size:
2790 1730 1139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2265 3014 844
Size: 1295 126 749 3489
Fragments arranged by size:
3489 1295 749 126
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 2479 1184
Size: 1295 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 2602 2602
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2214 2523 2688 2989 3356 3741 3758 5210
Size: 242 309 165 301 367 385 17 1452
Cuts at: 5210 5296 5308 5654 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 309 301 242
165 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2882 2882
Size: 5659
BtgI C'CryG_G
Cuts at: 513 1065 2226 2417 513
Size: 552 1161 191 3755
Fragments arranged by size:
3755 1161 552 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2263 487
Size: 683 56 1037 3883
Fragments arranged by size:
3883 1037 683 56
BtsI GCAGTG_nn'
Cuts at: 3175 3969 3989 3175
Size: 794 20 4845
Fragments arranged by size:
4845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2538 2573 529
Size: 35 1974 35 3615
Fragments arranged by size:
3615 1974 35 35
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2229 2250
Size: 54 1425 50 275 18 312 87 21
Cuts at: 2250 2376 2487 2592 2673 3106 3995 5624 8
Size: 126 111 105 81 433 889 1629 43
Fragments arranged by size:
1629 1425 889 433 312 275 126 111
105 87 81 54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2192 3589 1353
Size: 737 102 1397 3423
Fragments arranged by size:
3423 1397 737 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2173 2302 2721 2807 2860 2908
Size: 393 425 116 129 419 86 53 48
Cuts at: 2908 2918 3722 4319 5106 5374 1239
Size: 10 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 425 419 393 268
129 116 86 53 48 10
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2938 2935
Size: 3 5656
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 2275 4806 1678
Size: 597 2531 2531
Fragments arranged by size:
2531 2531 597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2246 2278 4300
Size: 32 135 32 430 32 212 32 2022
Cuts at: 4300 4332 4374 4406 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2022 430 212 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2687 4773 4957 1305
Size: 1382 2086 184 2007
Fragments arranged by size:
2086 2007 1382 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 2231 2378 2594 10
Size: 54 1425 343 312 87 147 216 3075
Fragments arranged by size:
3075 1425 343 312 216 147 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2354 2471 2747 2825 3617 3976
Size: 648 1011 177 117 276 78 792 359
Cuts at: 3976 4135 518
Size: 159 2042
Fragments arranged by size:
2042 1011 792 648 359 276 177 159
117 78
MspA1I CmG'CkG
Cuts at: 1606 2077 2419 3075 3752 4900 5145 1606
Size: 471 342 656 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 656 471 342 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5107
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2248 3017 4562 1132
Size: 97 1019 769 1545 2229
Fragments arranged by size:
2229 1545 1019 769 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2151 2809 1108
Size: 1043 658 3958
Fragments arranged by size:
3958 1043 658
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 3011 830
Size: 2181 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2077 2077
Size: 5659
SacII CC_GC'GG
Cuts at: 2420 2420
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SapI GCTCTTCn'nnn_
Cuts at: 2090 2090
Size: 5659
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 3007 4142 4823 5014 826
Size: 219 1962 1135 681 191 1471
Fragments arranged by size:
1962 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3763 4664 4926 820
Size: 197 926 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 2248 3017 1132
Size: 1116 769 3774
Fragments arranged by size:
3774 1116 769
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 2717 1517
Size: 1200 4459
StyI C'CwwG_G
Cuts at: 513 1065 3098 513
Size: 552 2033 3074
Fragments arranged by size:
3074 2033 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 2348 3686 3703 3856 4041 4486
Size: 1255 702 66 1338 17 153 185 445
Cuts at: 4486 325
Size: 1498
Fragments arranged by size:
1498 1338 1255 702 445 185 153 66
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2565 2597 5619 5651 2565
Size: 32 3022 32 2573
Fragments arranged by size:
3022 2573 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2284
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 2918 3788 2918
Size: 870 4789
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SanDI SexAI SfiI SfoI SrfI SwaI