Restriction map for
pFN21AE5432
(Circular) (Six-base) MAPSORT of: pFN21AE5432.seq Check: 5262 from: 1 to: 5671
pFN21AE5432 5671 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2720 2720
Size: 5671
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 2690 2915 3734 5118 5386 1632
Size: 404 654 225 819 1384 268 1917
Fragments arranged by size:
1917 1384 819 654 404 268 225
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 2831 1166
Size: 1665 4006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2396 1184
Size: 1212 4459
AlwNI CAG_nnn'CTG
Cuts at: 3414 4986 3414
Size: 1572 4099
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2008 2142 2980 3206 3358 5467 1493
Size: 515 134 838 226 152 2109 1697
Fragments arranged by size:
2109 1697 838 515 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 1870
Size: 73 1053 4545
Fragments arranged by size:
4545 1053 73
BamHI G'GATC_C
Cuts at: 2603 3001 3368 2603
Size: 398 367 4906
Fragments arranged by size:
4906 398 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2691 2992 4447 618
Size: 325 217 627 904 301 1455 1842
Fragments arranged by size:
1842 1455 904 627 325 301 217
BanII G_rGCy'C
Cuts at: 1391 2893 2990 1391
Size: 1502 97 4072
Fragments arranged by size:
4072 1502 97
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 2100 1548
Size: 552 5119
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4282 136
Size: 107 122 71 631 605 2610 1525
Fragments arranged by size:
2610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 2694 3732 4888 1790
Size: 904 1038 1156 2573
Fragments arranged by size:
2573 1156 1038 904
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3402 4360 442
Size: 416 53 83 399 24 1985 958 1753
Fragments arranged by size:
1985 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2108
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4331 1239
Size: 3092 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3796 4661 4959 3796
Size: 865 298 4508
Fragments arranged by size:
4508 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5446
Fragments arranged by size:
5446 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5671
BseYI C'CCAG_C
Cuts at: 4874 5370 4874
Size: 496 5175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2698 1163
Size: 573 962 4136
Fragments arranged by size:
4136 962 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3732 3817 4888 3732
Size: 85 1071 4515
Fragments arranged by size:
4515 1071 85
BsmI GAATG_Cn'
Cuts at: 2330 3182 3275 2330
Size: 852 93 4726
Fragments arranged by size:
4726 852 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2694 2893 2990 3732 3817 4888 1391
Size: 399 904 199 97 742 85 1071 2174
Fragments arranged by size:
2174 1071 904 742 399 199 97 85
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 2097 2926 3560 5290 2097
Size: 829 634 1730 2478
Fragments arranged by size:
2478 1730 829 634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2720 3026 844
Size: 1876 306 3489
Fragments arranged by size:
3489 1876 306
BsrBI CCG'CTC
Cuts at: 1394 1823 2036 3558 1394
Size: 429 213 1522 3507
Fragments arranged by size:
3507 1522 429 213
BsrDI GCAATG_nn'
Cuts at: 65 1248 2454 4166 4340 65
Size: 1183 1206 1712 174 1396
Fragments arranged by size:
1712 1396 1206 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4315 4502 1700
Size: 110 2505 187 2869
Fragments arranged by size:
2869 2505 187 110
BsrGI T'GTAC_A
Cuts at: 96 2823 96
Size: 2727 2944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 2734 1184
Size: 1550 4121
BstXI CCAn_nnnn'nTGG
Cuts at: 2614 2614
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2603 3001 3368 3753 3770 5222 5308 5320
Size: 631 398 367 385 17 1452 86 12
Cuts at: 5320 5666 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 631 398 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2867 2867
Size: 5671
BtgI C'CryG_G
Cuts at: 513 1065 2343 513
Size: 552 1278 3841
Fragments arranged by size:
3841 1278 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2378 3187 3981 4001 2378
Size: 809 794 20 4048
Fragments arranged by size:
4048 809 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DraIII CAC_nnn'GTG
Cuts at: 2625 2625
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3118 4007 5636
Size: 54 1425 50 275 18 1288 889 1629
Cuts at: 5636 8
Size: 43
Fragments arranged by size:
1629 1425 1288 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2498 2891 3601 1353
Size: 746 399 393 710 3423
Fragments arranged by size:
3423 746 710 399 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2690 2915 3734 4331 5118 5386
Size: 393 404 654 225 819 597 787 268
Cuts at: 5386 1239
Size: 1524
Fragments arranged by size:
1524 819 787 654 597 404 393 268
225
EcoRI G'AATT_C
Cuts at: 2008 2980 3358 2008
Size: 972 378 4321
Fragments arranged by size:
4321 972 378
EcoRV GAT'ATC
Cuts at: 2566 2566
Size: 5671
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 4818 1678
Size: 3140 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 4312 4344 4386
Size: 32 135 32 699 32 2009 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 2009 699 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4785 4969 1305
Size: 3480 184 2007
Fragments arranged by size:
3480 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3849
Fragments arranged by size:
3849 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2612 2768 2831 3629 3988 4147 518
Size: 648 1446 156 63 798 359 159 2042
Fragments arranged by size:
2042 1446 798 648 359 159 156 63
MspA1I CmG'CkG
Cuts at: 1606 2303 3087 3764 4912 5157 1606
Size: 697 784 677 1148 245 2120
Fragments arranged by size:
2120 1148 784 697 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2343 513
Size: 552 1278 3841
Fragments arranged by size:
3841 1278 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2236 3029 4574 1132
Size: 97 1007 793 1545 2229
Fragments arranged by size:
2229 1545 1007 793 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 3023 830
Size: 2193 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2860 3019 4154 4835 5026 826
Size: 219 1815 159 1135 681 191 1471
Fragments arranged by size:
1815 1471 1135 681 219 191 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3775 4676 4938 820
Size: 197 926 1832 901 262 1553
Fragments arranged by size:
1832 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 2702 1517
Size: 1185 4486
StyI C'CwwG_G
Cuts at: 513 1065 2275 2343 3110 513
Size: 552 1210 68 767 3074
Fragments arranged by size:
3074 1210 767 552 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2242 3698 3715 3868 4053 4498 325
Size: 1255 662 1456 17 153 185 445 1498
Fragments arranged by size:
1498 1456 1255 662 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 3917 5629
Size: 274 80 33 51 1443 846 1094 1712
Cuts at: 5629 96
Size: 138
Fragments arranged by size:
1712 1443 1094 846 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 2146 3800 2146
Size: 1654 4017
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI