Restriction map for
pFN21AE5430
(Circular) (Six-base) MAPSORT of: pFN21AE5430.seq Check: 7531 from: 1 to: 5662
pFN21AE5430 5662 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2735 2735
Size: 5662
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3005 1937
Size: 1068 4594
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3725 5109 5377 1632
Size: 2093 1384 268 1917
Fragments arranged by size:
2093 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 2747 1166
Size: 1581 4081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2622 2654 2622
Size: 32 5630
AlwNI CAG_nnn'CTG
Cuts at: 2451 3405 4977 2451
Size: 954 1572 3136
Fragments arranged by size:
3136 1572 954
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2971 3197 3349 5458 1493
Size: 1478 226 152 2109 1697
Fragments arranged by size:
2109 1697 1478 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2987 1870
Size: 73 1044 4545
Fragments arranged by size:
4545 1044 73
AvrII C'CTAG_G
Cuts at: 2194 2626 2194
Size: 432 5230
BamHI G'GATC_C
Cuts at: 2688 2992 3359 2688
Size: 304 367 4991
Fragments arranged by size:
4991 367 304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2983 4438 618
Size: 325 217 627 1196 1455 1842
Fragments arranged by size:
1842 1455 1196 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2981 1391
Size: 1590 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2656 2710 2749 928
Size: 971 757 54 39 3841
Fragments arranged by size:
3841 971 757 54 39
BbvCI CC'TCA_GC
Cuts at: 2695 2700 2695
Size: 5 5657
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 1903
Size: 34 1916 34 3678
Fragments arranged by size:
3678 1916 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 2409 2595 1548
Size: 861 186 4615
Fragments arranged by size:
4615 861 186
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4273 136
Size: 107 122 71 631 605 2601 1525
Fragments arranged by size:
2601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5657 5657
Size: 5662
BlpI GC'TnA_GC
Cuts at: 3079 3079
Size: 5662
Bme1580I G_kGCm'C
Cuts at: 1790 3723 4879 1790
Size: 1933 1156 2573
Fragments arranged by size:
2573 1933 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3393 4351 442
Size: 416 53 83 399 24 1976 958 1753
Fragments arranged by size:
1976 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4322 1239
Size: 3083 2579
Bpu10I CC'TnA_GC
Cuts at: 2351 2695 2700 2351
Size: 344 5 5313
Fragments arranged by size:
5313 344 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3787 4652 4950 3787
Size: 865 298 4499
Fragments arranged by size:
4499 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 2935 4325 882
Size: 617 516 920 1390 2219
Fragments arranged by size:
2219 1390 920 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2508 2538 2622 2652 1178
Size: 30 165 30 1105 30 84 30 4188
Fragments arranged by size:
4188 1105 165 84 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2224 2944 1186
Size: 1038 720 3904
Fragments arranged by size:
3904 1038 720
BseYI C'CCAG_C
Cuts at: 2125 4865 5361 2125
Size: 2740 496 2426
Fragments arranged by size:
2740 2426 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 2324 1163
Size: 573 420 168 4501
Fragments arranged by size:
4501 573 420 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 3723 3808 4879 2097
Size: 1626 85 1071 2880
Fragments arranged by size:
2880 1626 1071 85
BsmI GAATG_Cn'
Cuts at: 3173 3266 3173
Size: 93 5569
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2981 3723 3808 4879 1391
Size: 399 307 884 742 85 1071 2174
Fragments arranged by size:
2174 1071 884 742 399 307 85
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 2412 3551 5281 2412
Size: 1139 1730 2793
Fragments arranged by size:
2793 1730 1139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2735 3017 844
Size: 1891 282 3489
Fragments arranged by size:
3489 1891 282
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 4157 4331 65
Size: 1183 889 2020 174 1396
Fragments arranged by size:
2020 1396 1183 889 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4306 4493 1700
Size: 110 2496 187 2869
Fragments arranged by size:
2869 2496 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 2114 3722 4734 2114
Size: 1608 1012 3042
Fragments arranged by size:
3042 1608 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 2149 2866 1184
Size: 965 717 3980
Fragments arranged by size:
3980 965 717
BstYI r'GATC_y
Cuts at: 1972 2688 2827 2992 3359 3744 3761 5213 5299
Size: 716 139 165 367 385 17 1452 86
Cuts at: 5299 5311 5657 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 716 385 367 346 165 139
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2051 2396 2684 2900 2051
Size: 345 288 216 4813
Fragments arranged by size:
4813 345 288 216
BtgI C'CryG_G
Cuts at: 513 1065 2004 2325 2436 2475 2745 2808 513
Size: 552 939 321 111 39 270 63 3367
Fragments arranged by size:
3367 939 552 321 270 111 63 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2379 3109 3998
Size: 54 1425 50 275 18 549 730 889
Cuts at: 3998 5627 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 730 549 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3592 1353
Size: 2239 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3725 4322 5109 5377 1239
Size: 393 2093 597 787 268 1524
Fragments arranged by size:
2093 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2137 2766 2137
Size: 629 5033
EcoRI G'AATT_C
Cuts at: 2971 3349 2971
Size: 378 5284
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2846 2104
Size: 742 4920
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 4809 1678
Size: 3131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2508 2540 4303 4335 4377
Size: 32 135 32 936 32 1763 32 42
Cuts at: 4377 4409 1373
Size: 32 2626
Fragments arranged by size:
2626 1763 936 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3006 3252 669
Size: 988 281 1068 246 3079
Fragments arranged by size:
3079 1068 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4776 4960 1305
Size: 3471 184 2007
Fragments arranged by size:
3471 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 10
Size: 54 1425 343 549 3291
Fragments arranged by size:
3291 1425 549 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2180 2435 2591 2606 2747 3620
Size: 648 981 33 255 156 15 141 873
Cuts at: 3620 3979 4138 518
Size: 359 159 2042
Fragments arranged by size:
2042 981 873 648 359 255 159 156
141 33 15
MspA1I CmG'CkG
Cuts at: 1606 2503 2699 3078 3755 4903 5148 1606
Size: 897 196 379 677 1148 245 2120
Fragments arranged by size:
2120 1148 897 677 379 245 196
NcoI C'CATG_G
Cuts at: 513 1065 2004 2325 2436 2808 513
Size: 552 939 321 111 372 3367
Fragments arranged by size:
3367 939 552 372 321 111
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 2434 3020 4565 1132
Size: 97 1205 586 1545 2229
Fragments arranged by size:
2229 1545 1205 586 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5662
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2451 1108
Size: 1343 4319
PfoI T'CCnGG_A
Cuts at: 2211 2250 2211
Size: 39 5623
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2622 2654 3731 3763 5244 5276 2622
Size: 32 1077 32 1481 32 3008
Fragments arranged by size:
3008 1481 1077 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2766 2137
Size: 629 5033
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PstI C_TGCA'G
Cuts at: 830 3014 830
Size: 2184 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
PvuII CAG'CTG
Cuts at: 2503 2699 2503
Size: 196 5466
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 2893 3010 4145 4826 5017 826
Size: 219 1848 117 1135 681 191 1471
Fragments arranged by size:
1848 1471 1135 681 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3766 4667 4929 820
Size: 197 926 1823 901 262 1553
Fragments arranged by size:
1823 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 2004 2194 2325 2436 2490 2626 2808
Size: 552 939 190 131 111 54 136 182
Cuts at: 2808 3101 513
Size: 293 3074
Fragments arranged by size:
3074 939 552 293 190 182 136 131
111 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2082 3689 3706 3859 4044 4489 325
Size: 1255 502 1607 17 153 185 445 1498
Fragments arranged by size:
1607 1498 1255 502 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 3908 5620
Size: 274 80 33 51 1443 207 1724 1712
Cuts at: 5620 96
Size: 138
Fragments arranged by size:
1724 1712 1443 274 207 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5622 5654 5622
Size: 32 5630
Tth111I GACn'n_nGTC
Cuts at: 1316 2522 1316
Size: 1206 4456
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI