Restriction map for
pFN21AE5429
(Circular) (Six-base) MAPSORT of: pFN21AE5429.seq Check: 3145 from: 1 to: 5710
pFN21AE5429 5710 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2037 2037
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2503 3053 1937
Size: 566 550 4594
Fragments arranged by size:
4594 566 550
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2166 2388 2495 3773 5157 5425 1632
Size: 534 222 107 1278 1384 268 1917
Fragments arranged by size:
1917 1384 1278 534 268 222 107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 2986 3453 5025 2986
Size: 467 1572 3671
Fragments arranged by size:
3671 1572 467
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2055 2119 2793 2882 3019 3245 3397 5506
Size: 562 64 674 89 137 226 152 2109
Cuts at: 5506 1493
Size: 1697
Fragments arranged by size:
2109 1697 674 562 226 152 137 89
64
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BamHI G'GATC_C
Cuts at: 2873 3040 3407 2873
Size: 167 367 5176
Fragments arranged by size:
5176 367 167
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 3031 4486 618
Size: 325 217 627 224 1020 1455 1842
Fragments arranged by size:
1842 1455 1020 627 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2680 3029 1391
Size: 1289 349 4072
Fragments arranged by size:
4072 1289 349
BbeI G_GCGC'C
Cuts at: 2015 2015
Size: 5710
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 2961 1548
Size: 1413 4297
BfrBI ATG'CAT
Cuts at: 2903 2903
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4321 136
Size: 107 122 71 631 605 337 2312 1525
Fragments arranged by size:
2312 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 3771 4927 1790
Size: 1981 1156 2573
Fragments arranged by size:
2573 1981 1156
BmgBI CAC'GTC
Cuts at: 1318 2323 2967 1318
Size: 1005 644 4061
Fragments arranged by size:
4061 1005 644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2010 2257 3441
Size: 416 53 83 399 24 593 247 1184
Cuts at: 3441 4399 442
Size: 958 1753
Fragments arranged by size:
1753 1184 958 593 416 399 247 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2915 4370 1239
Size: 1676 1455 2579
Fragments arranged by size:
2579 1676 1455
Bpu10I CC'TnA_GC
Cuts at: 2199 2199
Size: 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2920 4373 882
Size: 617 1421 1453 2219
Fragments arranged by size:
2219 1453 1421 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2022 3517 3899
Size: 53 83 186 936 479 10 1495 382
Cuts at: 3899 4534 275
Size: 635 1451
Fragments arranged by size:
1495 1451 936 635 479 382 186 83
53 10
BsaWI w'CCGG_w
Cuts at: 2191 4143 4541 4815 4962 5373 2191
Size: 1952 398 274 147 411 2528
Fragments arranged by size:
2528 1952 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 2846 1186
Size: 1132 528 4050
Fragments arranged by size:
4050 1132 528
BseYI C'CCAG_C
Cuts at: 2336 4913 5409 2336
Size: 2577 496 2637
Fragments arranged by size:
2637 2577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2481 2952 3771 3856 4927 2481
Size: 471 819 85 1071 3264
Fragments arranged by size:
3264 1071 819 471 85
BsmI GAATG_Cn'
Cuts at: 2843 3221 3314 2843
Size: 378 93 5239
Fragments arranged by size:
5239 378 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2481 2680 2952 3029 3771 3856 4927
Size: 399 691 199 272 77 742 85 1071
Cuts at: 4927 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 691 399 272 199 85
77
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 3065 844
Size: 1193 1028 3489
Fragments arranged by size:
3489 1193 1028
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2602 4205 4379 65
Size: 1183 1354 1603 174 1396
Fragments arranged by size:
1603 1396 1354 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2167 4354 4541 1700
Size: 110 357 2187 187 2869
Fragments arranged by size:
2869 2187 357 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 2976 1925
Size: 1051 4659
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2796 2796
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2768 2873 2982 3040 3407 3792 3809 5261
Size: 796 105 109 58 367 385 17 1452
Cuts at: 5261 5347 5359 5705 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 796 385 367 346 109 105
86 58 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2563 2826 513
Size: 552 939 559 263 3397
Fragments arranged by size:
3397 939 559 552 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 2349 2887 3014 2349
Size: 538 127 5045
Fragments arranged by size:
5045 538 127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675
Size: 54 1425 50 275 18 1327 889 1629
Cuts at: 5675 8
Size: 43
Fragments arranged by size:
1629 1425 1327 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2068 2336 2364 2514 3640 1353
Size: 715 268 28 150 1126 3423
Fragments arranged by size:
3423 1126 715 268 150 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2388 2495 2915 3773 4370 5157
Size: 393 534 222 107 420 858 597 787
Cuts at: 5157 5425 1239
Size: 268 1524
Fragments arranged by size:
1524 858 787 597 534 420 393 268
222 107
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 2015 4857 1678
Size: 337 2842 2531
Fragments arranged by size:
2842 2531 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2365 2397 2511 2543 4351
Size: 32 135 32 793 32 114 32 1808
Cuts at: 4351 4383 4425 4457 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1808 793 135 114 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2108 2356 2504 3054 3300 669
Size: 988 281 170 248 148 550 246 3079
Fragments arranged by size:
3079 988 550 281 248 246 170 148
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KasI G'GCGC_C
Cuts at: 2011 2011
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 2655 3309 2655
Size: 654 5056
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2724 3668 4027 4186 518
Size: 648 1558 944 359 159 2042
Fragments arranged by size:
2042 1558 944 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2311 3126 3803 4951 5196 1606
Size: 705 815 677 1148 245 2120
Fragments arranged by size:
2120 1148 815 705 677 245
NaeI GCC'GGC
Cuts at: 2169 2169
Size: 5710
NarI GG'CG_CC
Cuts at: 2012 2012
Size: 5710
NcoI C'CATG_G
Cuts at: 513 1065 2004 2563 2826 513
Size: 552 939 559 263 3397
Fragments arranged by size:
3397 939 559 552 263
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NgoMIV G'CCGG_C
Cuts at: 2167 2167
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NsiI A_TGCA'T
Cuts at: 2905 2905
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PfoI T'CCnGG_A
Cuts at: 2892 2892
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2912 1613
Size: 1299 4411
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2176 3062 830
Size: 1346 886 3478
Fragments arranged by size:
3478 1346 886
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2311 2311
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 2502 3052 1936
Size: 566 550 4594
Fragments arranged by size:
4594 566 550
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2172 2850 3058 4193 4874 5065 826
Size: 219 1127 678 208 1135 681 191 1471
Fragments arranged by size:
1471 1135 1127 681 678 219 208 191
SfoI GGC'GCC
Cuts at: 2013 2013
Size: 5710
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3814 4715 4977 820
Size: 197 926 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2563 2826 3149 513
Size: 552 939 559 263 323 3074
Fragments arranged by size:
3074 939 559 552 323 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3737 3754 3907 4092 4537 325
Size: 1255 2157 17 153 185 445 1498
Fragments arranged by size:
2157 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NruI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI