Restriction map for
pFN21AE5422
(Circular) (Six-base) MAPSORT of: pFN21AE5422.seq Check: 2685 from: 1 to: 5701
pFN21AE5422 5701 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2282 2282
Size: 5701
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 3044 1937
Size: 807 300 4594
Fragments arranged by size:
4594 807 300
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2183 2198 2657 2756 3764 5148 5416
Size: 515 36 15 459 99 1008 1384 268
Cuts at: 5416 1632
Size: 1917
Fragments arranged by size:
1917 1384 1008 515 459 268 99 36
15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 4600 5577 1203
Size: 3397 977 1327
Fragments arranged by size:
3397 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AlwNI CAG_nnn'CTG
Cuts at: 3444 5016 3444
Size: 1572 4129
ApaLI G'TGCA_C
Cuts at: 2813 3758 4914 2813
Size: 945 1156 3600
Fragments arranged by size:
3600 1156 945
ApoI r'AATT_y
Cuts at: 1493 2400 2929 3010 3236 3388 5497 1493
Size: 907 529 81 226 152 2109 1697
Fragments arranged by size:
2109 1697 907 529 226 152 81
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 3026 1870
Size: 73 1083 4545
Fragments arranged by size:
4545 1083 73
BamHI G'GATC_C
Cuts at: 2786 3031 3398 2786
Size: 245 367 5089
Fragments arranged by size:
5089 367 245
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 3022 4477 618
Size: 325 217 627 303 932 1455 1842
Fragments arranged by size:
1842 1455 932 627 325 303 217
BanII G_rGCy'C
Cuts at: 1391 3020 1391
Size: 1629 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2645 928
Size: 971 746 3984
Fragments arranged by size:
3984 971 746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3892 3926 1903
Size: 34 1955 34 3678
Fragments arranged by size:
3678 1955 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2885 3593 4803 2885
Size: 708 1210 3783
Fragments arranged by size:
3783 1210 708
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4312 136
Size: 107 122 71 631 605 2640 1525
Fragments arranged by size:
2640 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2601 2817 3762 4918 1790
Size: 811 216 945 1156 2573
Fragments arranged by size:
2573 1156 945 811 216
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 2813 3432
Size: 416 53 83 399 24 639 757 619
Cuts at: 3432 4390 442
Size: 958 1753
Fragments arranged by size:
1753 958 757 639 619 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2254 4361 1239
Size: 1015 2107 2579
Fragments arranged by size:
2579 2107 1015
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4691 4989 3826
Size: 865 298 4538
Fragments arranged by size:
4538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4364 882
Size: 617 2865 2219
Fragments arranged by size:
2865 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3492 493
Size: 711 2288 2702
Fragments arranged by size:
2702 2288 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3508 3890 4525 275
Size: 53 83 186 936 1975 382 635 1451
Fragments arranged by size:
1975 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4134 4532 4806 4953 5364 4134
Size: 398 274 147 411 4471
Fragments arranged by size:
4471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5476
Fragments arranged by size:
5476 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2951 2954 1186
Size: 1765 3 3933
Fragments arranged by size:
3933 1765 3
BseYI C'CCAG_C
Cuts at: 4904 5400 4904
Size: 496 5205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2982 1163
Size: 573 1246 3882
Fragments arranged by size:
3882 1246 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3151 3912 4940 1540
Size: 275 186 1150 761 1028 2301
Fragments arranged by size:
2301 1150 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2817 3762 3847 4918 2817
Size: 945 85 1071 3600
Fragments arranged by size:
3600 1071 945 85
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2601 2817 3020 3762 3847 4918 1391
Size: 399 811 216 203 742 85 1071 2174
Fragments arranged by size:
2174 1071 811 742 399 216 203 85
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2282 3056 844
Size: 1438 774 3489
Fragments arranged by size:
3489 1438 774
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 1394
Size: 429 1765 3507
Fragments arranged by size:
3507 1765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4196 4370 65
Size: 1183 2948 174 1396
Fragments arranged by size:
2948 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4345 4532 1700
Size: 110 2535 187 2869
Fragments arranged by size:
2869 2535 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 2472 3761 4773 2472
Size: 1289 1012 3400
Fragments arranged by size:
3400 1289 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5701
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5701
BstYI r'GATC_y
Cuts at: 1972 2194 2776 2786 2989 3031 3398 3783 3800
Size: 222 582 10 203 42 367 385 17
Cuts at: 3800 5252 5338 5350 5696 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 582 385 367 346 222 203
86 42 17 12 10
BstZ17I GTA'TAC
Cuts at: 2745 2745
Size: 5701
BtgI C'CryG_G
Cuts at: 513 1065 2560 2829 513
Size: 552 1495 269 3385
Fragments arranged by size:
3385 1495 552 269
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 3217 4011 4031 3217
Size: 794 20 4887
Fragments arranged by size:
4887 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2403 2438 529
Size: 35 1839 35 3792
Fragments arranged by size:
3792 1839 35 35
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3148 4037 5666
Size: 54 1425 50 275 18 1318 889 1629
Cuts at: 5666 8
Size: 43
Fragments arranged by size:
1629 1425 1318 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2951 3631 1353
Size: 1598 680 3423
Fragments arranged by size:
3423 1598 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2908 4288 4662 4808 387
Size: 1064 138 1319 1380 374 146 1280
Fragments arranged by size:
1380 1319 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2183 2198 2254 2657 2756 3764
Size: 393 515 36 15 56 403 99 1008
Cuts at: 3764 4361 5148 5416 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1008 787 597 515 403 393 268
99 56 36 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2655 2655
Size: 5701
EcoO109I rG'GnC_Cy
Cuts at: 2944 2944
Size: 5701
EcoRI G'AATT_C
Cuts at: 2929 3010 3388 2929
Size: 81 378 5242
Fragments arranged by size:
5242 378 81
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 4848 1678
Size: 3170 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 4342 4374 4416
Size: 32 135 32 470 32 2268 32 42
Cuts at: 4416 4448 1373
Size: 32 2626
Fragments arranged by size:
2626 2268 470 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 3045 3291 669
Size: 988 281 72 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246 72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2062 2359 2546 4815 4999 1305
Size: 757 297 187 2269 184 2007
Fragments arranged by size:
2269 2007 757 297 187 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3879
Fragments arranged by size:
3879 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3659 4018 4177 518
Size: 648 2493 359 159 2042
Fragments arranged by size:
2493 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3117 3794 4942 5187 1606
Size: 1511 677 1148 245 2120
Fragments arranged by size:
2120 1511 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2560 2829 513
Size: 552 1495 269 3385
Fragments arranged by size:
3385 1495 552 269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 3059 4604 1132
Size: 97 1830 1545 2229
Fragments arranged by size:
2229 1830 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5701
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2853 2885 3770 3802 5283 5315 2853
Size: 32 885 32 1481 32 3239
Fragments arranged by size:
3239 1481 885 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 3271 3271
Size: 5701
PstI C_TGCA'G
Cuts at: 830 3053 830
Size: 2223 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 2224 3949 5661 2224
Size: 1725 1712 2264
Fragments arranged by size:
2264 1725 1712
SfcI C'TryA_G
Cuts at: 826 1045 3049 4184 4865 5056 826
Size: 219 2004 1135 681 191 1471
Fragments arranged by size:
2004 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 3028 1872
Size: 1156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3805 4706 4968 820
Size: 197 926 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SspI AAT'ATT
Cuts at: 5 52 2980 2997 3625 5
Size: 47 2928 17 628 2081
Fragments arranged by size:
2928 2081 628 47 17
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5701
StyI C'CwwG_G
Cuts at: 513 1065 2560 2829 3140 513
Size: 552 1495 269 311 3074
Fragments arranged by size:
3074 1495 552 311 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2528 2832 3728 3745 3898 4083 4528
Size: 1255 948 304 896 17 153 185 445
Cuts at: 4528 325
Size: 1498
Fragments arranged by size:
1498 1255 948 896 445 304 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2222 3947 5659
Size: 274 80 33 51 1443 245 1725 1712
Cuts at: 5659 96
Size: 138
Fragments arranged by size:
1725 1712 1443 274 245 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2440 2472 2754 2786 2807 2839 5661 5693 2440
Size: 32 282 32 21 32 2822 32 2448
Fragments arranged by size:
2822 2448 282 32 32 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 2497 3037 2497
Size: 540 5161
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 3026 1870
Size: 1156 4545
XmnI GAAnn'nnTTC
Cuts at: 2665 2865 3830 2665
Size: 200 965 4536
Fragments arranged by size:
4536 965 200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI