Restriction map for
pFN21AE5420
(Circular) (Six-base) MAPSORT of: pFN21AE5420.seq Check: 5571 from: 1 to: 5650
pFN21AE5420 5650 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2277 2672 3713 5097 5365 1632
Size: 645 395 1041 1384 268 1917
Fragments arranged by size:
1917 1384 1041 645 395 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 2402 4379 2402
Size: 1977 3673
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 3393 4965 3393
Size: 1572 4078
ApaLI G'TGCA_C
Cuts at: 2594 3707 4863 2594
Size: 1113 1156 3381
Fragments arranged by size:
3381 1156 1113
ApoI r'AATT_y
Cuts at: 1493 2959 3185 3337 5446 1493
Size: 1466 226 152 2109 1697
Fragments arranged by size:
2109 1697 1466 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2495 2736 2808 2975 1870
Size: 73 552 241 72 167 4545
Fragments arranged by size:
4545 552 241 167 73 72
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2008 2041 2008
Size: 33 5617
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2364 2730 2969 1391
Size: 973 366 239 4072
Fragments arranged by size:
4072 973 366 239
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2314 928
Size: 971 415 4264
Fragments arranged by size:
4264 971 415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2214 2224 2248 2258 3841 3875 1903
Size: 34 277 10 24 10 1583 34 3678
Fragments arranged by size:
3678 1583 277 34 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 2036 3542 4752 2036
Size: 1506 1210 2934
Fragments arranged by size:
2934 1506 1210
BclI T'GATC_A
Cuts at: 1548 2037 1548
Size: 489 5161
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2598 3711 4867 1790
Size: 303 505 1113 1156 2573
Fragments arranged by size:
2573 1156 1113 505 303
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2299 4310 1239
Size: 1060 2011 2579
Fragments arranged by size:
2579 2011 1060
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4640 4938 3775
Size: 865 298 4487
Fragments arranged by size:
4487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2484 2733 4313 882
Size: 617 985 249 1580 2219
Fragments arranged by size:
2219 1580 985 617 249
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 2036 5644 2036
Size: 3608 2042
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2697 4083 4481 4755 4902 5313 2697
Size: 1386 398 274 147 411 3034
Fragments arranged by size:
3034 1386 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2831 1186
Size: 1645 4005
BseYI C'CCAG_C
Cuts at: 4853 5349 4853
Size: 496 5154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2143 1163
Size: 573 407 4670
Fragments arranged by size:
4670 573 407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 3100 3861 4889 1540
Size: 275 186 853 246 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 275 246 186
BsiHKAI G_wGCw'C
Cuts at: 2598 3711 3796 4867 2598
Size: 1113 85 1071 3381
Fragments arranged by size:
3381 1113 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2364 2598 2730 2969 3711 3796
Size: 399 303 271 234 132 239 742 85
Cuts at: 3796 4867 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 271 239 234
132 85
BspEI T'CCGG_A
Cuts at: 2697 5313 2697
Size: 2616 3034
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2210 3005 844
Size: 1366 795 3489
Fragments arranged by size:
3489 1366 795
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2079 4145 4319 65
Size: 1183 831 2066 174 1396
Fragments arranged by size:
2066 1396 1183 831 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2086
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2732 2980 3347 3732 3749 5201 5287 5299
Size: 760 248 367 385 17 1452 86 12
Cuts at: 5299 5645 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 760 385 367 346 248 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2829 513
Size: 552 1764 3334
Fragments arranged by size:
3334 1764 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2551 487
Size: 683 56 1325 3586
Fragments arranged by size:
3586 1325 683 56
BtsI GCAGTG_nn'
Cuts at: 2091 2909 3166 3960 3980 2091
Size: 818 257 794 20 3761
Fragments arranged by size:
3761 818 794 257 20
ClaI AT'CG_AT
Cuts at: 1554 2244 2692 1554
Size: 690 448 4512
Fragments arranged by size:
4512 690 448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2925 3097 3986
Size: 54 1425 50 275 18 1095 172 889
Cuts at: 3986 5615 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1095 889 275 172 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3580 1353
Size: 2227 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 2299 2672 3713 4310 5097 5365
Size: 393 645 22 373 1041 597 787 268
Cuts at: 5365 1239
Size: 1524
Fragments arranged by size:
1524 1041 787 645 597 393 373 268
22
EcoO109I rG'GnC_Cy
Cuts at: 2199 2310 2812 2835 2199
Size: 111 502 23 5014
Fragments arranged by size:
5014 502 111 23
EcoRI G'AATT_C
Cuts at: 2959 3337 2959
Size: 378 5272
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2388 2420 2717 2749 4291
Size: 32 135 32 816 32 297 32 1542
Cuts at: 4291 4323 4365 4397 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1542 816 297 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2874 4764 4948 1305
Size: 1569 1890 184 2007
Fragments arranged by size:
2007 1890 1569 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3828
Fragments arranged by size:
3828 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2084 2792 3608 3967 4126 518
Size: 648 843 75 708 816 359 159 2042
Fragments arranged by size:
2042 843 816 708 648 359 159 75
MspA1I CmG'CkG
Cuts at: 1606 3066 3743 4891 5136 1606
Size: 1460 677 1148 245 2120
Fragments arranged by size:
2120 1460 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2829 513
Size: 552 1764 3334
Fragments arranged by size:
3334 1764 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 3008 4553 1132
Size: 97 1779 1545 2229
Fragments arranged by size:
2229 1779 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5650
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2199 2812 2835 2199
Size: 613 23 5014
Fragments arranged by size:
5014 613 23
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2440 3002 830
Size: 1610 562 3478
Fragments arranged by size:
3478 1610 562
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SanDI GG'GwC_CC
Cuts at: 2812 2835 2812
Size: 23 5627
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2046 2436 2683 2870 2998 4133 4814
Size: 219 1001 390 247 187 128 1135 681
Cuts at: 4814 5005 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1001 681 390 247 219 191
187 128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2497 2810 2977 1872
Size: 625 313 167 4545
Fragments arranged by size:
4545 625 313 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3754 4655 4917 820
Size: 197 926 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2829 3089 513
Size: 552 1764 260 3074
Fragments arranged by size:
3074 1764 552 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2905 3677 3694 3847 4032 4477 325
Size: 1255 1325 772 17 153 185 445 1498
Fragments arranged by size:
1498 1325 1255 772 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3896 5608 96
Size: 274 80 33 51 1443 1919 1712 138
Fragments arranged by size:
1919 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2495 2808 2975 1870
Size: 625 313 167 4545
Fragments arranged by size:
4545 625 313 167
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI