Restriction map for
pFN21AE5414
(Circular) (Six-base) MAPSORT of: pFN21AE5414.seq Check: 478 from: 1 to: 5752
pFN21AE5414 5752 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2352 3095 1937
Size: 415 743 4594
Fragments arranged by size:
4594 743 415
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 2857 2945 3815 5199 5467 1632
Size: 431 794 88 870 1384 268 1917
Fragments arranged by size:
1917 1384 870 794 431 268 88
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 2515 4651 5628 1203
Size: 1312 2136 977 1327
Fragments arranged by size:
2136 1327 1312 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 2096 3495 5067 2096
Size: 1399 1572 2781
Fragments arranged by size:
2781 1572 1399
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2396 2916 3041 3061 3287 3439 5548 1493
Size: 903 520 125 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 903 520 226 152 125 20
AseI AT'TA_AT
Cuts at: 160 1032 2598 4306 160
Size: 872 1566 1708 1606
Fragments arranged by size:
1708 1606 1566 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 3015 3077 1870
Size: 73 1072 62 4545
Fragments arranged by size:
4545 1072 73 62
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3071 1391
Size: 1680 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2678 928
Size: 971 779 4002
Fragments arranged by size:
4002 971 779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3019 3644 4854 3019
Size: 625 1210 3917
Fragments arranged by size:
3917 1210 625
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4363 136
Size: 107 122 71 631 605 2691 1525
Fragments arranged by size:
2691 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 3813 4969 1790
Size: 2023 1156 2573
Fragments arranged by size:
2573 2023 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4412 1239
Size: 3173 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2961 4415 882
Size: 617 1462 1454 2219
Fragments arranged by size:
2219 1462 1454 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 2438 5746 2438
Size: 3308 2444
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 3941 4576 275
Size: 53 83 186 936 2026 382 635 1451
Fragments arranged by size:
2026 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5527
Fragments arranged by size:
5527 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5752
BseYI C'CCAG_C
Cuts at: 2096 2767 4955 5451 2096
Size: 671 2188 496 2397
Fragments arranged by size:
2397 2188 671 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3813 3898 4969 3813
Size: 85 1071 4596
Fragments arranged by size:
4596 1071 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3071 3813 3898 4969 1391
Size: 399 1281 742 85 1071 2174
Fragments arranged by size:
2174 1281 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2824 3107 844
Size: 1980 283 3489
Fragments arranged by size:
3489 1980 283
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 3639 1394
Size: 429 205 1611 3507
Fragments arranged by size:
3507 1611 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747
Size: 1110 367 385 17 1452 86 12 346
Cuts at: 5747 1972
Size: 1977
Fragments arranged by size:
1977 1452 1110 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4261
Fragments arranged by size:
4261 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2551 487
Size: 683 56 1325 3688
Fragments arranged by size:
3688 1325 683 56
BtsI GCAGTG_nn'
Cuts at: 2683 3268 4062 4082 2683
Size: 585 794 20 4353
Fragments arranged by size:
4353 794 585 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 2668 3056 2668
Size: 388 5364
DraIII CAC_nnn'GTG
Cuts at: 2211 2211
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3199 4088 5717
Size: 54 1425 50 275 18 1369 889 1629
Cuts at: 5717 8
Size: 43
Fragments arranged by size:
1629 1425 1369 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 2524 2701 2964 3682 1353
Size: 1008 163 177 263 718 3423
Fragments arranged by size:
3423 1008 718 263 177 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2139 4339 4713 4859 387
Size: 1064 138 550 2200 374 146 1280
Fragments arranged by size:
2200 1280 1064 550 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2857 2945 3815 4412 5199 5467
Size: 393 431 794 88 870 597 787 268
Cuts at: 5467 1239
Size: 1524
Fragments arranged by size:
1524 870 794 787 597 431 393 268
88
EcoO109I rG'GnC_Cy
Cuts at: 2716 2716
Size: 5752
EcoRI G'AATT_C
Cuts at: 2916 3061 3439 2916
Size: 145 378 5229
Fragments arranged by size:
5229 378 145
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2097 4899 1678
Size: 419 2802 2531
Fragments arranged by size:
2802 2531 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373
Size: 32 135 32 2821 32 42 32 2626
Fragments arranged by size:
2821 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 2778 3351 2778
Size: 573 5179
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2149 4866 5050 1305
Size: 844 2717 184 2007
Fragments arranged by size:
2717 2007 844 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3710 4069 4228 518
Size: 648 2544 359 159 2042
Fragments arranged by size:
2544 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2066 2188 2227 3168 3845 4993 5238
Size: 431 29 122 39 941 677 1148 245
Cuts at: 5238 1606
Size: 2120
Fragments arranged by size:
2120 1148 941 677 431 245 122 39
29
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4261
Fragments arranged by size:
4261 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2519 3110 4655 1132
Size: 97 1290 591 1545 2229
Fragments arranged by size:
2229 1545 1290 591 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 2515 4651 2515
Size: 2136 3616
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2716 2716
Size: 5752
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 2092 3104 830
Size: 1262 1012 3478
Fragments arranged by size:
3478 1262 1012
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 2088 2388 3100 4235 4916 5107 826
Size: 219 1043 300 712 1135 681 191 1471
Fragments arranged by size:
1471 1135 1043 712 681 300 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3017 3079 1872
Size: 1145 62 4545
Fragments arranged by size:
4545 1145 62
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 2004 3191 513
Size: 552 939 1187 3074
Fragments arranged by size:
3074 1187 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3796 3949 4134 4579 325
Size: 1255 2199 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3998 5710 96
Size: 274 80 33 51 1443 2021 1712 138
Fragments arranged by size:
2021 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3015 3077 1870
Size: 1145 62 4545
Fragments arranged by size:
4545 1145 62
XmnI GAAnn'nnTTC
Cuts at: 2076 2742 3881 2076
Size: 666 1139 3947
Fragments arranged by size:
3947 1139 666
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI