Restriction map for
pFN21AE5410
(Circular) (Six-base) MAPSORT of: pFN21AE5410.seq Check: 6633 from: 1 to: 5641
pFN21AE5410 5641 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2502 2506 2502
Size: 4 5637
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2772 2984 1937
Size: 835 212 4594
Fragments arranged by size:
4594 835 212
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaI G_GGCC'C
Cuts at: 2261 2261
Size: 5641
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 2966 1870
Size: 73 116 907 4545
Fragments arranged by size:
4545 907 116 73
BamHI G'GATC_C
Cuts at: 2971 3338 2971
Size: 367 5274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 4417 618
Size: 325 217 627 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2261 2931 2960 1391
Size: 870 670 29 4072
Fragments arranged by size:
4072 870 670 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2083 2273 2574 2602 928
Size: 971 184 190 301 28 3967
Fragments arranged by size:
3967 971 301 190 184 28
BbvCI CC'TCA_GC
Cuts at: 2675 2675
Size: 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2298 5636 2298
Size: 3338 2303
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 2261 2843 3702 4858 1790
Size: 471 582 859 1156 2573
Fragments arranged by size:
2573 1156 859 582 471
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2762 4301 1239
Size: 1523 1539 2579
Fragments arranged by size:
2579 1539 1523
Bpu10I CC'TnA_GC
Cuts at: 2675 2675
Size: 5641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2828 4304 882
Size: 617 1329 1476 2219
Fragments arranged by size:
2219 1476 1329 617
BsaAI yAC'GTr
Cuts at: 493 1204 2368 3432 493
Size: 711 1164 1064 2702
Fragments arranged by size:
2702 1164 1064 711
BsaBI GATnn'nnATC
Cuts at: 2303 5635 2303
Size: 3332 2309
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 1178
Size: 30 165 30 600 30 4786
Fragments arranged by size:
4786 600 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2713 1186
Size: 1527 4114
BseYI C'CCAG_C
Cuts at: 2542 2911 4844 5340 2542
Size: 369 1933 496 2843
Fragments arranged by size:
2843 1933 496 369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2474 1163
Size: 573 738 4330
Fragments arranged by size:
4330 738 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2512 3091 3852 4880 1540
Size: 275 186 511 579 761 1028 2301
Fragments arranged by size:
2301 1028 761 579 511 275 186
BsiHKAI G_wGCw'C
Cuts at: 3702 3787 4858 3702
Size: 85 1071 4485
Fragments arranged by size:
4485 1071 85
BsmI GAATG_Cn'
Cuts at: 2897 3152 3245 2897
Size: 255 93 5293
Fragments arranged by size:
5293 255 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2843 2931 2960 3702 3787 4858
Size: 399 471 582 88 29 742 85 1071
Cuts at: 4858 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 582 471 399 88 85
29
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2502 2506 2726 2996 844
Size: 1658 4 220 270 3489
Fragments arranged by size:
3489 1658 270 220 4
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2763 4136 4310 65
Size: 1183 1515 1373 174 1396
Fragments arranged by size:
1515 1396 1373 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 2221 3701 4713 2221
Size: 1480 1012 3149
Fragments arranged by size:
3149 1480 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2740 2887 1184
Size: 1556 147 3938
Fragments arranged by size:
3938 1556 147
BstYI r'GATC_y
Cuts at: 1972 2298 2586 2971 3338 3723 3740 5192 5278
Size: 326 288 385 367 385 17 1452 86
Cuts at: 5278 5290 5636 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 385 367 346 326 288
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2873 2873
Size: 5641
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2676 2841 3157 3951 3971 2676
Size: 165 316 794 20 4346
Fragments arranged by size:
4346 794 316 165 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2291 4648 809
Size: 507 975 2357 1802
Fragments arranged by size:
2357 1802 975 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3088 3977 5606
Size: 54 1425 50 275 18 1258 889 1629
Cuts at: 5606 8
Size: 43
Fragments arranged by size:
1629 1425 1258 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2753 2831 3571 1353
Size: 731 669 78 740 3423
Fragments arranged by size:
3423 740 731 669 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2713 4228 4602 4748 387
Size: 1064 138 1124 1515 374 146 1280
Fragments arranged by size:
1515 1280 1124 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2762 3704 4301 5088 5356 1239
Size: 393 1130 942 597 787 268 1524
Fragments arranged by size:
1524 1130 942 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2257 2539 2715 2257
Size: 282 176 5183
Fragments arranged by size:
5183 282 176
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373
Size: 32 135 32 2710 32 42 32 2626
Fragments arranged by size:
2710 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2011 2248 2985 3231 669
Size: 988 281 73 237 737 246 3079
Fragments arranged by size:
3079 988 737 281 246 237 73
HindIII A'AGCT_T
Cuts at: 748 2706 748
Size: 1958 3683
HpaI GTT'AAC
Cuts at: 2011 3231 2011
Size: 1220 4421
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2214 4755 4939 1305
Size: 909 2541 184 2007
Fragments arranged by size:
2541 2007 909 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3599 3958 4117 518
Size: 648 2433 359 159 2042
Fragments arranged by size:
2433 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2428 3057 3734 4882 5127 1606
Size: 822 629 677 1148 245 2120
Fragments arranged by size:
2120 1148 822 677 629 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2317 1348
Size: 969 4672
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 2664 1108
Size: 1134 422 4085
Fragments arranged by size:
4085 1134 422
PfoI T'CCnGG_A
Cuts at: 2280 2280
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2539 2715 2539
Size: 176 5465
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspOMI G'GGCC_C
Cuts at: 2257 2257
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2512 2001
Size: 511 5130
PvuII CAG'CTG
Cuts at: 2428 2428
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2752 2989 4124 4805 4996 826
Size: 219 1707 237 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 681 237 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2061 2968 1872
Size: 189 907 4545
Fragments arranged by size:
4545 907 189
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3745 4646 4908 820
Size: 197 926 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 3565 5
Size: 47 3513 2081
Fragments arranged by size:
3513 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 3080 513
Size: 552 939 1076 3074
Fragments arranged by size:
3074 1076 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 3887 5599
Size: 274 80 33 51 1443 198 1712 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1712 1443 274 198 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2016 2048 2118 2150 5601 5633 2016
Size: 32 70 32 3451 32 2024
Fragments arranged by size:
3451 2024 70 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2278
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2059 2966 1870
Size: 189 907 4545
Fragments arranged by size:
4545 907 189
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI