Restriction map for
pFN21AE5407
(Circular) (Six-base) MAPSORT of: pFN21AE5407.seq Check: 3174 from: 1 to: 5698
pFN21AE5407 5698 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2088 2088
Size: 5698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2576 3761 5145 5413 1632
Size: 497 447 1185 1384 268 1917
Fragments arranged by size:
1917 1384 1185 497 447 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 2765 1166
Size: 1599 4099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2783 1184
Size: 1599 4099
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaI G_GGCC'C
Cuts at: 2171 2960 2171
Size: 789 4909
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 2663 3007 3233 3385 5494 1493
Size: 1170 344 226 152 2109 1697
Fragments arranged by size:
2109 1697 1170 344 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 2902 2959 3023 1870
Size: 73 116 843 57 64 4545
Fragments arranged by size:
4545 843 116 73 64 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2202 2235 2202
Size: 33 5665
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3019 4474 618
Size: 325 217 627 1232 1455 1842
Fragments arranged by size:
1842 1455 1232 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2171 2903 2960 2966 3017 1391
Size: 780 732 57 6 51 4072
Fragments arranged by size:
4072 780 732 57 51 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2459 2564 928
Size: 971 560 105 4062
Fragments arranged by size:
4062 971 560 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2080 3590 4800 2080
Size: 1510 1210 2978
Fragments arranged by size:
2978 1510 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BfrBI ATG'CAT
Cuts at: 2689 2689
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2062 2406 4309
Size: 107 122 71 631 605 390 344 1903
Cuts at: 4309 136
Size: 1525
Fragments arranged by size:
1903 1525 631 605 390 344 122 107
71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 2171 2809 2960 3759 4915 1790
Size: 381 638 151 799 1156 2573
Fragments arranged by size:
2573 1156 799 638 381 151
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2815 2922 3429
Size: 416 53 83 399 24 1398 107 507
Cuts at: 3429 4387 442
Size: 958 1753
Fragments arranged by size:
1753 1398 958 507 416 399 107 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2331 2906 2999 4358 1239
Size: 1092 575 93 1359 2579
Fragments arranged by size:
2579 1359 1092 575 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 2917 3823 4688 4986 2255
Size: 662 906 865 298 2967
Fragments arranged by size:
2967 906 865 662 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2120 4361 882
Size: 617 621 2241 2219
Fragments arranged by size:
2241 2219 621 617
BsaAI yAC'GTr
Cuts at: 493 1204 2470 3489 493
Size: 711 1266 1019 2702
Fragments arranged by size:
2702 1266 1019 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2659 4131 4529 4803 4950 5361 2659
Size: 1472 398 274 147 411 2996
Fragments arranged by size:
2996 1472 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2304 2334 1178
Size: 30 165 30 901 30 4542
Fragments arranged by size:
4542 901 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 1186
Size: 1153 4545
BseYI C'CCAG_C
Cuts at: 2164 4901 5397 2164
Size: 2737 496 2465
Fragments arranged by size:
2737 2465 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2699 2743 1163
Size: 573 963 44 4118
Fragments arranged by size:
4118 963 573 44
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2966 3759 3844 4915 2325
Size: 641 793 85 1071 3108
Fragments arranged by size:
3108 1071 793 641 85
BsmI GAATG_Cn'
Cuts at: 2258 2493 3209 3302 2258
Size: 235 716 93 4654
Fragments arranged by size:
4654 716 235 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2687 2687
Size: 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2171 2325 2809 2903 2960 2966 3017
Size: 399 381 154 484 94 57 6 51
Cuts at: 3017 3759 3844 4915 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 399 381 154 94
85 57 51 6
BspEI T'CCGG_A
Cuts at: 2659 5361 2659
Size: 2702 2996
BspHI T'CATG_A
Cuts at: 2722 3587 5317 2722
Size: 865 1730 3103
Fragments arranged by size:
3103 1730 865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2986 3053 844
Size: 1244 898 67 3489
Fragments arranged by size:
3489 1244 898 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2138 3585 1394
Size: 429 315 1447 3507
Fragments arranged by size:
3507 1447 429 315
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 2361 96
Size: 2265 3433
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2640 2686 794
Size: 1846 46 3806
Fragments arranged by size:
3806 1846 46
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 2309
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693
Size: 1056 367 385 17 1452 86 12 346
Cuts at: 5693 1972
Size: 1977
Fragments arranged by size:
1977 1452 1056 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2813 2868 2813
Size: 55 5643
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 513
Size: 552 939 168 4039
Fragments arranged by size:
4039 939 552 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2687 2748 3214 4008 4028 2687
Size: 61 466 794 20 4357
Fragments arranged by size:
4357 794 466 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2499 2580 3145
Size: 54 1425 50 275 18 669 81 565
Cuts at: 3145 4034 5663 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 669 565 275 81 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 2885 3628 1353
Size: 828 704 743 3423
Fragments arranged by size:
3423 828 743 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2964 2964
Size: 5698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2331 2576 2906 2999 3761 4358
Size: 393 497 202 245 330 93 762 597
Cuts at: 4358 5145 5413 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 762 597 497 393 330 268
245 202 93
EcoNI CCTnn'n_nnAGG
Cuts at: 2672 2672
Size: 5698
EcoO109I rG'GnC_Cy
Cuts at: 2168 2527 2817 2827 2956 2168
Size: 359 290 10 129 4910
Fragments arranged by size:
4910 359 290 129 10
EcoRI G'AATT_C
Cuts at: 2663 3007 3385 2663
Size: 344 378 4976
Fragments arranged by size:
4976 378 344
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2331 2363 2580 2612 4339
Size: 32 135 32 759 32 217 32 1727
Cuts at: 4339 4371 4413 4445 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1727 759 217 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 2568 4812 4996 1305
Size: 969 294 2244 184 2007
Fragments arranged by size:
2244 2007 969 294 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3876
Fragments arranged by size:
3876 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2245 2357 2765 3656 4015 4174 518
Size: 648 1079 112 408 891 359 159 2042
Fragments arranged by size:
2042 1079 891 648 408 359 159 112
MspA1I CmG'CkG
Cuts at: 1606 3114 3791 4939 5184 1606
Size: 1508 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2172 513
Size: 552 939 168 4039
Fragments arranged by size:
4039 939 552 168
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NsiI A_TGCA'T
Cuts at: 2691 2691
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2689 3056 4601 1132
Size: 97 1460 367 1545 2229
Fragments arranged by size:
2229 1545 1460 367 97
PasI CC'CwG_GG
Cuts at: 1348 2953 1348
Size: 1605 4093
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 1108
Size: 1134 4564
PfoI T'CCnGG_A
Cuts at: 2841 2932 2841
Size: 91 5607
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PmlI CAC'GTG
Cuts at: 2470 2470
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2817 2827 2817
Size: 10 5688
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PspOMI G'GGCC_C
Cuts at: 2167 2956 2167
Size: 789 4909
PstI C_TGCA'G
Cuts at: 830 2881 3050 830
Size: 2051 169 3478
Fragments arranged by size:
3478 2051 169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
SacI G_AGCT'C
Cuts at: 2966 2966
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SapI GCTCTTCn'nnn_
Cuts at: 2885 2885
Size: 5698
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 2316 3946 5658 2316
Size: 1630 1712 2356
Fragments arranged by size:
2356 1712 1630
SfcI C'TryA_G
Cuts at: 826 1045 2415 2877 3046 4181 4862 5053 826
Size: 219 1370 462 169 1135 681 191 1471
Fragments arranged by size:
1471 1370 1135 681 462 219 191 169
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 2061 2904 3025 1872
Size: 189 843 121 4545
Fragments arranged by size:
4545 843 189 121
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2270 2896 3802 4703 4965 820
Size: 197 926 327 626 906 901 262 1553
Fragments arranged by size:
1553 926 906 901 626 327 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 2689 3056 1132
Size: 1557 367 3774
Fragments arranged by size:
3774 1557 367
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 2570 2592 1517
Size: 1053 22 4623
Fragments arranged by size:
4623 1053 22
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 2379 2509 2970 3137 513
Size: 552 939 168 207 130 461 167 3074
Fragments arranged by size:
3074 939 552 461 207 168 167 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2777 3725 3742 3895 4080 4525 325
Size: 1255 1197 948 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1197 948 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2314 2361 2538
Size: 274 80 33 51 1443 337 47 177
Cuts at: 2538 3944 5656 96
Size: 1406 1712 138
Fragments arranged by size:
1712 1443 1406 337 274 177 138 80
51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2304 2336 5658 5690 2304
Size: 32 3322 32 2312
Fragments arranged by size:
3322 2312 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2273 2482 2273
Size: 209 5489
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 2059 2902 3023 1870
Size: 189 843 121 4545
Fragments arranged by size:
4545 843 189 121
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI