Restriction map for
pFN21AE5404
(Circular) (Six-base) MAPSORT of: pFN21AE5404.seq Check: 8296 from: 1 to: 5635
pFN21AE5404 5635 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2016 3445 278
Size: 53 83 186 936 480 1429 2468
Fragments arranged by size:
2468 1429 936 480 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2933 3698 5082 5350 1632
Size: 1301 765 1384 268 1917
Fragments arranged by size:
1917 1384 1301 765 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 2680 3378 4950 2680
Size: 698 1572 3365
Fragments arranged by size:
3365 1572 698
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2382 2944 3170 3322 5431 1493
Size: 889 562 226 152 2109 1697
Fragments arranged by size:
2109 1697 889 562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2960 1870
Size: 73 1017 4545
Fragments arranged by size:
4545 1017 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2870 2956 4411 618
Size: 325 217 627 1083 86 1455 1842
Fragments arranged by size:
1842 1455 1083 627 325 217 86
BanII G_rGCy'C
Cuts at: 1391 2954 1391
Size: 1563 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 2160 1548
Size: 612 5023
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2066 4246 136
Size: 107 122 71 631 605 394 2180 1525
Fragments arranged by size:
2180 1525 631 605 394 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 3696 4852 1790
Size: 1906 1156 2573
Fragments arranged by size:
2573 1906 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3366 4324 442
Size: 416 53 83 399 24 1949 958 1753
Fragments arranged by size:
1949 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2210 1056
Size: 1154 4481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2071 2187 4295 1239
Size: 832 116 2108 2579
Fragments arranged by size:
2579 2108 832 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2543 4298 882
Size: 617 1044 1755 2219
Fragments arranged by size:
2219 1755 1044 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 3442 3824 4459
Size: 53 83 186 936 480 1429 382 635
Cuts at: 4459 275
Size: 1451
Fragments arranged by size:
1451 1429 936 635 480 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2443 4068 4466 4740 4887 5298 2443
Size: 1625 398 274 147 411 2780
Fragments arranged by size:
2780 1625 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2465 2495 1178
Size: 30 165 30 1062 30 4318
Fragments arranged by size:
4318 1062 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2311 1186
Size: 1125 4510
BseYI C'CCAG_C
Cuts at: 4838 5334 4838
Size: 496 5139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5062
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3696 3781 4852 3696
Size: 85 1071 4479
Fragments arranged by size:
4479 1071 85
BsmI GAATG_Cn'
Cuts at: 2409 3146 3239 2409
Size: 737 93 4805
Fragments arranged by size:
4805 737 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2954 3696 3781 4852 1391
Size: 399 1164 742 85 1071 2174
Fragments arranged by size:
2174 1164 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2990 844
Size: 2146 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2284 3522 1394
Size: 429 461 1238 3507
Fragments arranged by size:
3507 1238 461 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2265 2712 4130 4304 65
Size: 1183 1017 447 1418 174 1396
Fragments arranged by size:
1418 1396 1183 1017 447 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4279 4466 1700
Size: 110 2469 187 2869
Fragments arranged by size:
2869 2469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2965 3332 3717 3734 5186 5272 5284 5630
Size: 993 367 385 17 1452 86 12 346
Cuts at: 5630 1972
Size: 1977
Fragments arranged by size:
1977 1452 993 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2190 487
Size: 683 56 964 3932
Fragments arranged by size:
3932 964 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 3151 3945 3965 2163
Size: 988 794 20 3833
Fragments arranged by size:
3833 988 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DraIII CAC_nnn'GTG
Cuts at: 2902 2902
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3082 3971 5600
Size: 54 1425 50 275 18 1252 889 1629
Cuts at: 5600 8
Size: 43
Fragments arranged by size:
1629 1425 1252 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3565 1353
Size: 2212 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2187 2933 3698 4295 5082 5350
Size: 393 439 116 746 765 597 787 268
Cuts at: 5350 1239
Size: 1524
Fragments arranged by size:
1524 787 765 746 597 439 393 268
116
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 2878 2642
Size: 236 5399
EcoO109I rG'GnC_Cy
Cuts at: 2635 2635
Size: 5635
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2378 2149
Size: 229 5406
FspI TGC'GCA
Cuts at: 2623 4141 2623
Size: 1518 4117
FspAI rTGC'GCAy
Cuts at: 2623 2623
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 4782 1678
Size: 3104 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2701 2733 4276 4308 4350
Size: 32 135 32 1129 32 1543 32 42
Cuts at: 4350 4382 1373
Size: 32 2626
Fragments arranged by size:
2626 1543 1129 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2392 2979 3225 669
Size: 988 281 454 587 246 3079
Fragments arranged by size:
3079 988 587 454 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 2784 3234 2784
Size: 450 5185
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2380 4749 4933 1305
Size: 1075 2369 184 2007
Fragments arranged by size:
2369 2007 1075 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3813
Fragments arranged by size:
3813 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2675 3593 3952 4111 518
Size: 648 1509 918 359 159 2042
Fragments arranged by size:
2042 1509 918 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2868 3051 3728 4876 5121 1606
Size: 1262 183 677 1148 245 2120
Fragments arranged by size:
2120 1262 1148 677 245 183
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
NdeI CA'TA_TG
Cuts at: 387 2517 2689 387
Size: 2130 172 3333
Fragments arranged by size:
3333 2130 172
NheI G'CTAG_C
Cuts at: 1052 2206 1052
Size: 1154 4481
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2993 4538 1132
Size: 97 1764 1545 2229
Fragments arranged by size:
2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PfoI T'CCnGG_A
Cuts at: 2016 2016
Size: 5635
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2289 2863 2983 4118 4799 4990 826
Size: 219 1244 574 120 1135 681 191 1471
Fragments arranged by size:
1471 1244 1135 681 574 219 191 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2004 2576 2607 2631 2925 3074 513
Size: 552 939 572 31 24 294 149 3074
Fragments arranged by size:
3074 939 572 552 294 149 31 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3662 3679 3832 4017 4462 325
Size: 1255 2082 17 153 185 445 1498
Fragments arranged by size:
2082 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 3881 5593
Size: 274 80 33 51 1443 618 1286 1712
Cuts at: 5593 96
Size: 138
Fragments arranged by size:
1712 1443 1286 618 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5595 5627 5595
Size: 32 5603
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 3764 3764
Size: 5635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2014 3443 276
Size: 53 83 186 936 480 1429 2468
Fragments arranged by size:
2468 1429 936 480 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI