Restriction map for
pFN21AE5403
(Circular) (Six-base) MAPSORT of: pFN21AE5403.seq Check: 2271 from: 1 to: 5725
pFN21AE5403 5725 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2956 2956
Size: 5725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3788 5172 5440 1632
Size: 2156 1384 268 1917
Fragments arranged by size:
2156 1917 1384 268
AfeI AGC'GCT
Cuts at: 2250 2250
Size: 5725
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 2289 4556 2289
Size: 2267 3458
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 2168 1166
Size: 1002 4723
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2367 1184
Size: 1183 4542
AlwNI CAG_nnn'CTG
Cuts at: 2086 3468 5040 2086
Size: 1382 1572 2771
Fragments arranged by size:
2771 1572 1382
ApaI G_GGCC'C
Cuts at: 2024 2024
Size: 5725
ApaLI G'TGCA_C
Cuts at: 2342 3782 4938 2342
Size: 1440 1156 3129
Fragments arranged by size:
3129 1440 1156
ApoI r'AATT_y
Cuts at: 1493 2590 3034 3260 3412 5521 1493
Size: 1097 444 226 152 2109 1697
Fragments arranged by size:
2109 1697 1097 444 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2261 3050 1870
Size: 73 80 238 789 4545
Fragments arranged by size:
4545 789 238 80 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2232 3046 4501 618
Size: 325 217 627 445 814 1455 1842
Fragments arranged by size:
1842 1455 814 627 445 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2043 2196 3044 1391
Size: 633 19 153 848 4072
Fragments arranged by size:
4072 848 633 153 19
BbeI G_GCGC'C
Cuts at: 2236 2236
Size: 5725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2405 928
Size: 971 506 4248
Fragments arranged by size:
4248 971 506
BbvCI CC'TCA_GC
Cuts at: 2322 2322
Size: 5725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2515 2586 3617 4827 2515
Size: 71 1031 1210 3413
Fragments arranged by size:
3413 1210 1031 71
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 2042 3142 2042
Size: 1100 4625
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2346 3786 4942 1790
Size: 234 322 1440 1156 2573
Fragments arranged by size:
2573 1440 1156 322 234
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3456 4414 442
Size: 416 53 83 399 24 2039 958 1753
Fragments arranged by size:
2039 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2808 2808
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 4385 1239
Size: 1184 1962 2579
Fragments arranged by size:
2579 1962 1184
Bpu10I CC'TnA_GC
Cuts at: 2322 2322
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2583 3850 4715 5013 2583
Size: 1267 865 298 3295
Fragments arranged by size:
3295 1267 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2233 3532 3914 4549
Size: 53 83 186 936 700 1299 382 635
Cuts at: 4549 275
Size: 1451
Fragments arranged by size:
1451 1299 936 700 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2289 4158 4556 4830 4977 5388 2289
Size: 1869 398 274 147 411 2626
Fragments arranged by size:
2626 1869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5500
Fragments arranged by size:
5500 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 1186
Size: 1018 4707
BseYI C'CCAG_C
Cuts at: 2051 2195 4928 5424 2051
Size: 144 2733 496 2352
Fragments arranged by size:
2733 2352 496 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 2695 1163
Size: 573 378 581 4193
Fragments arranged by size:
4193 581 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2127 2169 3175 3936 4964 1540
Size: 275 186 126 42 1006 761 1028 2301
Fragments arranged by size:
2301 1028 1006 761 275 186 126 42
BsiHKAI G_wGCw'C
Cuts at: 2043 2346 2927 3786 3871 4942 2043
Size: 303 581 859 85 1071 2826
Fragments arranged by size:
2826 1071 859 581 303 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
BsmBI CGTCTCn'nnnn_
Cuts at: 2258 2258
Size: 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2043 2196 2346 2927 3044 3786
Size: 399 234 19 153 150 581 117 742
Cuts at: 3786 3871 4942 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 581 399 234 153 150
117 85 19
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 2394 3614 5344 2394
Size: 1220 1730 2775
Fragments arranged by size:
2775 1730 1220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2956 3080 844
Size: 2112 124 3489
Fragments arranged by size:
3489 2112 124
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2511 4220 4394 65
Size: 1183 1263 1709 174 1396
Fragments arranged by size:
1709 1396 1263 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2289 4369 4556 1700
Size: 110 479 2080 187 2869
Fragments arranged by size:
2869 2080 479 187 110
BsrGI T'GTAC_A
Cuts at: 96 2798 2821 96
Size: 2702 23 3000
Fragments arranged by size:
3000 2702 23
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 2692 4529 2692
Size: 1837 3888
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 2224 3055 3422 3807 3824 5276 5362 5374
Size: 252 831 367 385 17 1452 86 12
Cuts at: 5374 5720 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 831 385 367 346 252 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2147 2147
Size: 5725
BtgI C'CryG_G
Cuts at: 513 1065 2121 513
Size: 552 1056 4117
Fragments arranged by size:
4117 1056 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3241 4035 4055 2890
Size: 351 794 20 4560
Fragments arranged by size:
4560 794 351 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2166 2373
Size: 54 1425 50 275 18 294 42 207
Cuts at: 2373 3172 4061 5690 8
Size: 799 889 1629 43
Fragments arranged by size:
1629 1425 889 799 294 275 207 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2124 2166 3172 1537
Size: 275 312 42 1006 4090
Fragments arranged by size:
4090 1006 312 275 42
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 3655 1353
Size: 890 1412 3423
Fragments arranged by size:
3423 1412 890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2041 2041
Size: 5725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 3788 4385 5172 5440 1239
Size: 393 791 1365 597 787 268 1524
Fragments arranged by size:
1524 1365 791 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2210 2210
Size: 5725
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2058 2236 2252 4872 1678
Size: 380 178 16 2620 2531
Fragments arranged by size:
2620 2531 380 178 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373
Size: 32 135 32 2794 32 42 32 2626
Fragments arranged by size:
2794 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2617 3069 3315 669
Size: 988 281 679 452 246 3079
Fragments arranged by size:
3079 988 679 452 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KasI G'GCGC_C
Cuts at: 2232 2232
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 4839 5023 1305
Size: 684 2850 184 2007
Fragments arranged by size:
2850 2007 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 3360
Fragments arranged by size:
3360 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2349 3683 4042 4201 518
Size: 648 1002 181 1334 359 159 2042
Fragments arranged by size:
2042 1334 1002 648 359 181 159
MspA1I CmG'CkG
Cuts at: 1606 2070 2123 3141 3818 4966 5211 1606
Size: 464 53 1018 677 1148 245 2120
Fragments arranged by size:
2120 1148 1018 677 464 245 53
NarI GG'CG_CC
Cuts at: 2233 2233
Size: 5725
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 3083 4628 1132
Size: 97 1854 1545 2229
Fragments arranged by size:
2229 1854 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2506 1108
Size: 1398 4327
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 2541 3295 2541
Size: 754 4971
PspOMI G'GGCC_C
Cuts at: 2020 2020
Size: 5725
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2070 2070
Size: 5725
SacI G_AGCT'C
Cuts at: 2043 2043
Size: 5725
SacII CC_GC'GG
Cuts at: 2124 2124
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2733 3073 4208 4889 5080 826
Size: 219 1688 340 1135 681 191 1471
Fragments arranged by size:
1688 1471 1135 681 340 219 191
SfoI GGC'GCC
Cuts at: 2234 2234
Size: 5725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2598 3829 4730 4992 820
Size: 197 926 655 1231 901 262 1553
Fragments arranged by size:
1553 1231 926 901 655 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 2991 3649 5
Size: 47 2939 658 2081
Fragments arranged by size:
2939 2081 658 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 3164 513
Size: 552 2099 3074
Fragments arranged by size:
3074 2099 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 3752 3769 3922 4107 4552 325
Size: 1255 717 1455 17 153 185 445 1498
Fragments arranged by size:
1498 1455 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2798 2821 3971
Size: 274 80 33 51 1443 821 23 1150
Cuts at: 3971 5683 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1150 821 274 138 80 51
33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2502 2534 5685 5717 2502
Size: 32 3151 32 2510
Fragments arranged by size:
3151 2510 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI