Restriction map for
pFN21AE5402
(Circular) (Six-base) MAPSORT of: pFN21AE5402.seq Check: 6614 from: 1 to: 5644
pFN21AE5402 5644 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2376 2987 1937
Size: 439 611 4594
Fragments arranged by size:
4594 611 439
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3707 5091 5359 1632
Size: 2075 1384 268 1917
Fragments arranged by size:
2075 1917 1384 268
AfeI AGC'GCT
Cuts at: 2402 2402
Size: 5644
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 2685 2850 4543 5520 1203
Size: 1482 165 1693 977 1327
Fragments arranged by size:
1693 1482 1327 977 165
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 2294 2461 2884 3387 4959 2294
Size: 167 423 503 1572 2979
Fragments arranged by size:
2979 1572 503 423 167
ApaI G_GGCC'C
Cuts at: 2601 2601
Size: 5644
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2703 2953 3179 3331 5440 1493
Size: 1210 250 226 152 2109 1697
Fragments arranged by size:
2109 1697 1210 250 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2969 1870
Size: 73 1026 4545
Fragments arranged by size:
4545 1026 73
BamHI G'GATC_C
Cuts at: 2346 2932 2974 3341 2346
Size: 586 42 367 4649
Fragments arranged by size:
4649 586 367 42
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 4420 618
Size: 325 217 627 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2601 2963 1391
Size: 1210 362 4072
Fragments arranged by size:
4072 1210 362
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2500 2903 928
Size: 971 601 403 3669
Fragments arranged by size:
3669 971 601 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3835 3869 1903
Size: 34 50 34 1814 34 3678
Fragments arranged by size:
3678 1814 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2584 3536 4746 2584
Size: 952 1210 3482
Fragments arranged by size:
3482 1210 952
BclI T'GATC_A
Cuts at: 1548 2145 1548
Size: 597 5047
BfrBI ATG'CAT
Cuts at: 2468 2791 2468
Size: 323 5321
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4255 136
Size: 107 122 71 631 605 2583 1525
Fragments arranged by size:
2583 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2311 5639 2311
Size: 3328 2316
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 2601 3705 4861 1790
Size: 811 1104 1156 2573
Fragments arranged by size:
2573 1156 1104 811
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2056 2228 2276 4304 1239
Size: 817 172 48 2028 2579
Fragments arranged by size:
2579 2028 817 172 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2307 3769 4634 4932 2307
Size: 1462 865 298 3019
Fragments arranged by size:
3019 1462 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4307 882
Size: 617 2808 2219
Fragments arranged by size:
2808 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2415 3435 493
Size: 711 1211 1020 2702
Fragments arranged by size:
2702 1211 1020 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2427 2637 4077 4475 4749 4896 5307 2427
Size: 210 1440 398 274 147 411 2764
Fragments arranged by size:
2764 1440 411 398 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5419
Fragments arranged by size:
5419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5644
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2615 1163
Size: 573 879 4192
Fragments arranged by size:
4192 879 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3705 3790 4861 3705
Size: 85 1071 4488
Fragments arranged by size:
4488 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3248 3155
Size: 93 5551
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2601 2963 3705 3790 4861 1391
Size: 399 811 362 742 85 1071 2174
Fragments arranged by size:
2174 1071 811 742 399 362 85
BspEI T'CCGG_A
Cuts at: 2427 2637 5307 2427
Size: 210 2670 2764
Fragments arranged by size:
2764 2670 210
BspHI T'CATG_A
Cuts at: 3533 5263 3533
Size: 1730 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2999 844
Size: 2155 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2938 3531 1394
Size: 429 1115 593 3507
Fragments arranged by size:
3507 1115 593 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4139 4313 65
Size: 1183 2891 174 1396
Fragments arranged by size:
2891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5644
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2615 2809 794
Size: 1821 194 3629
Fragments arranged by size:
3629 1821 194
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstXI CCAn_nnnn'nTGG
Cuts at: 2717 2717
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2311 2346 2932 2974 3341 3726 3743 5195
Size: 339 35 586 42 367 385 17 1452
Cuts at: 5195 5281 5293 5639 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 586 385 367 346 339 86
42 35 17 12
Bsu36I CC'TnA_GG
Cuts at: 2454 2454
Size: 5644
BtgI C'CryG_G
Cuts at: 513 1065 2586 513
Size: 552 1521 3571
Fragments arranged by size:
3571 1521 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2628 487
Size: 683 56 1402 3503
Fragments arranged by size:
3503 1402 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 3160 3954 3974 2148
Size: 1012 794 20 3818
Fragments arranged by size:
3818 1012 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3091 3980 5609
Size: 54 1425 50 275 18 1261 889 1629
Cuts at: 5609 8
Size: 43
Fragments arranged by size:
1629 1425 1261 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2228 2276 3707 4304 5091 5359
Size: 393 424 172 48 1431 597 787 268
Cuts at: 5359 1239
Size: 1524
Fragments arranged by size:
1524 1431 787 597 424 393 268 172
48
EcoNI CCTnn'n_nnAGG
Cuts at: 2054 2812 2054
Size: 758 4886
EcoO109I rG'GnC_Cy
Cuts at: 2597 2598 2817 2597
Size: 1 219 5424
Fragments arranged by size:
5424 219 1
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 2404 4791 1678
Size: 726 2387 2531
Fragments arranged by size:
2531 2387 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 2901 2933 4285
Size: 32 135 32 704 32 593 32 1352
Cuts at: 4285 4317 4359 4391 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1352 704 593 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2988 3234 669
Size: 988 281 1050 246 3079
Fragments arranged by size:
3079 1050 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2624 748
Size: 1876 3768
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4758 4942 1305
Size: 3453 184 2007
Fragments arranged by size:
3453 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 2577 3602 3961 4120 518
Size: 648 1281 130 1025 359 159 2042
Fragments arranged by size:
2042 1281 1025 648 359 159 130
MspA1I CmG'CkG
Cuts at: 1606 2881 3060 3737 4885 5130 1606
Size: 1275 179 677 1148 245 2120
Fragments arranged by size:
2120 1275 1148 677 245 179
NcoI C'CATG_G
Cuts at: 513 1065 2586 513
Size: 552 1521 3571
Fragments arranged by size:
3571 1521 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NsiI A_TGCA'T
Cuts at: 2470 2793 2470
Size: 323 5321
NspI r_CATG'y
Cuts at: 1132 1229 2689 2854 3002 4547 1132
Size: 97 1460 165 148 1545 2229
Fragments arranged by size:
2229 1545 1460 165 148 97
PasI CC'CwG_GG
Cuts at: 1348 2746 1348
Size: 1398 4246
PciI A'CATG_T
Cuts at: 2685 2850 4543 2685
Size: 165 1693 3786
Fragments arranged by size:
3786 1693 165
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2884 1108
Size: 1776 3868
PfoI T'CCnGG_A
Cuts at: 2190 2190
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2817 2817
Size: 5644
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PspOMI G'GGCC_C
Cuts at: 2597 2597
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2996 830
Size: 2166 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
PvuII CAG'CTG
Cuts at: 2881 2881
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 2222 3892 5604 2222
Size: 1670 1712 2262
Fragments arranged by size:
2262 1712 1670
SfcI C'TryA_G
Cuts at: 826 1045 2992 4127 4808 4999 826
Size: 219 1947 1135 681 191 1471
Fragments arranged by size:
1947 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2322 3748 4649 4911 820
Size: 197 926 379 1426 901 262 1553
Fragments arranged by size:
1553 1426 926 901 379 262 197
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 2586 2716 2905 3083 513
Size: 552 1521 130 189 178 3074
Fragments arranged by size:
3074 1521 552 189 178 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2378 2759 3671 3688 3841 4026 4471
Size: 1255 798 381 912 17 153 185 445
Cuts at: 4471 325
Size: 1498
Fragments arranged by size:
1498 1255 912 798 445 381 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 3890 5602
Size: 274 80 33 51 1443 243 1670 1712
Cuts at: 5602 96
Size: 138
Fragments arranged by size:
1712 1670 1443 274 243 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2195 2227 5604 5636 2195
Size: 32 3377 32 2203
Fragments arranged by size:
3377 2203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5644
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 2184 2507 3773 2184
Size: 323 1266 4055
Fragments arranged by size:
4055 1266 323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI