Restriction map for
pFN21AE5396
(Circular) (Six-base) MAPSORT of: pFN21AE5396.seq Check: 4888 from: 1 to: 5632
pFN21AE5396 5632 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 3695 5079 5347 1632
Size: 551 1512 1384 268 1917
Fragments arranged by size:
1917 1512 1384 551 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 2714 2725 4531 5508 1203
Size: 1511 11 1806 977 1327
Fragments arranged by size:
1806 1511 1327 977 11
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 2255 1166
Size: 1089 4543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2081 1184
Size: 897 4735
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaI G_GGCC'C
Cuts at: 2511 2511
Size: 5632
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2953 4408 618
Size: 325 217 627 222 944 1455 1842
Fragments arranged by size:
1842 1455 944 627 325 222 217
BanII G_rGCy'C
Cuts at: 1391 2511 2951 1391
Size: 1120 440 4072
Fragments arranged by size:
4072 1120 440
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2331 5627 2331
Size: 3296 2336
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2511 3693 4849 1790
Size: 721 1182 1156 2573
Fragments arranged by size:
2573 1182 1156 721
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2432 1056
Size: 1376 4256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4292 1239
Size: 3053 2579
Bpu10I CC'TnA_GC
Cuts at: 2186 2920 2186
Size: 734 4898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 3757 4622 4920 2537
Size: 1220 865 298 3249
Fragments arranged by size:
3249 1220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 4295 882
Size: 617 1092 1704 2219
Fragments arranged by size:
2219 1704 1092 617
BsaAI yAC'GTr
Cuts at: 493 1204 2728 3423 493
Size: 711 1524 695 2702
Fragments arranged by size:
2702 1524 711 695
BsaBI GATnn'nnATC
Cuts at: 2541 5626 2541
Size: 3085 2547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 2929 1186
Size: 1285 458 3889
Fragments arranged by size:
3889 1285 458
BseYI C'CCAG_C
Cuts at: 2295 4835 5331 2295
Size: 2540 496 2596
Fragments arranged by size:
2596 2540 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 2586 1163
Size: 573 636 214 4209
Fragments arranged by size:
4209 636 573 214
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 3693 3778 4849 2109
Size: 1584 85 1071 2892
Fragments arranged by size:
2892 1584 1071 85
BsmI GAATG_Cn'
Cuts at: 2461 3143 3236 2461
Size: 682 93 4857
Fragments arranged by size:
4857 682 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2511 2951 3693 3778 4849 1391
Size: 399 319 402 440 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 440 402 399 319 85
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 844
Size: 2143 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2694 4276 4463 1700
Size: 110 884 1582 187 2869
Fragments arranged by size:
2869 1582 884 187 110
BsrGI T'GTAC_A
Cuts at: 96 2769 96
Size: 2673 2959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstXI CCAn_nnnn'nTGG
Cuts at: 2318 2791 2318
Size: 473 5159
BstYI r'GATC_y
Cuts at: 1972 2331 2962 3329 3714 3731 5183 5269 5281
Size: 359 631 367 385 17 1452 86 12
Cuts at: 5281 5627 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 631 385 367 359 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 3796
Fragments arranged by size:
3796 939 552 345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2164 4639 809
Size: 507 848 2475 1802
Fragments arranged by size:
2475 1802 848 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 2598 3079
Size: 54 1425 50 275 18 540 228 481
Cuts at: 3079 3968 5597 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 540 481 275 228 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 1353
Size: 2209 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 3695 4292 5079 5347 1239
Size: 393 551 1512 597 787 268 1524
Fragments arranged by size:
1524 1512 787 597 551 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2382 2436 2382
Size: 54 5578
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 5632
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 4779 1678
Size: 3101 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373
Size: 32 135 32 2701 32 42 32 2626
Fragments arranged by size:
2701 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2976 3222 669
Size: 988 281 1038 246 3079
Fragments arranged by size:
3079 1038 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 2600 10
Size: 54 1425 343 540 228 3042
Fragments arranged by size:
3042 1425 540 343 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 2576 2594 2765 2828 3590 3949
Size: 648 1089 321 18 171 63 762 359
Cuts at: 3949 4108 518
Size: 159 2042
Fragments arranged by size:
2042 1089 762 648 359 321 171 159
63 18
MspA1I CmG'CkG
Cuts at: 1606 2494 2607 3048 3725 4873 5118 1606
Size: 888 113 441 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 677 441 245 113
NcoI C'CATG_G
Cuts at: 513 1065 2004 2349 513
Size: 552 939 345 3796
Fragments arranged by size:
3796 939 552 345
NdeI CA'TA_TG
Cuts at: 387 2210 387
Size: 1823 3809
NheI G'CTAG_C
Cuts at: 1052 2428 1052
Size: 1376 4256
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2079 2718 2990 4535 1132
Size: 97 850 639 272 1545 2229
Fragments arranged by size:
2229 1545 850 639 272 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 2714 4531 2714
Size: 1817 3815
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspOMI G'GGCC_C
Cuts at: 2507 2507
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2475 2984 830
Size: 1645 509 3478
Fragments arranged by size:
3478 1645 509
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2494 2494
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2377 2471 2980 4115 4796 4987 826
Size: 219 1332 94 509 1135 681 191 1471
Fragments arranged by size:
1471 1332 1135 681 509 219 191 94
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 3736 4637 4899 820
Size: 197 926 609 1184 901 262 1553
Fragments arranged by size:
1553 1184 926 901 609 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2004 2349 3071 513
Size: 552 939 345 722 3074
Fragments arranged by size:
3074 939 722 552 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3659 3676 3829 4014 4459 325
Size: 1255 417 1662 17 153 185 445 1498
Fragments arranged by size:
1662 1498 1255 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 3878 5590
Size: 274 80 33 51 1443 792 1109 1712
Cuts at: 5590 96
Size: 138
Fragments arranged by size:
1712 1443 1109 792 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2318 2318
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI