Restriction map for
pFN21AE5393
(Circular) (Six-base) MAPSORT of: pFN21AE5393.seq Check: 5021 from: 1 to: 5632
pFN21AE5393 5632 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 2931 3759 4132 1563
Size: 1368 828 373 3063
Fragments arranged by size:
3063 1368 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2927 3695 5079 5347 1632
Size: 1295 768 1384 268 1917
Fragments arranged by size:
1917 1384 1295 768 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 2658 2723 4531 5508 1203
Size: 1455 65 1808 977 1327
Fragments arranged by size:
1808 1455 1327 977 65
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2254 2941 3167 3319 5428 1493
Size: 761 687 226 152 2109 1697
Fragments arranged by size:
2109 1697 761 687 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2953 4408 618
Size: 325 217 627 1166 1455 1842
Fragments arranged by size:
1842 1455 1166 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2951 1391
Size: 1560 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2710 2824 928
Size: 971 811 114 3736
Fragments arranged by size:
3736 971 811 114
BbvCI CC'TCA_GC
Cuts at: 2697 2697
Size: 5632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2152 3524 4734 2152
Size: 1372 1210 3050
Fragments arranged by size:
3050 1372 1210
BclI T'GATC_A
Cuts at: 1548 2844 1548
Size: 1296 4336
BfrBI ATG'CAT
Cuts at: 2291 2689 2291
Size: 398 5234
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 3693 4849 1790
Size: 1903 1156 2573
Fragments arranged by size:
2573 1903 1156
BmgBI CAC'GTC
Cuts at: 1318 2724 1318
Size: 1406 4226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2315 3363 4321
Size: 416 53 83 399 24 898 1048 958
Cuts at: 4321 442
Size: 1753
Fragments arranged by size:
1753 1048 958 898 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2400 2864 4292 1239
Size: 1161 464 1428 2579
Fragments arranged by size:
2579 1428 1161 464
Bpu10I CC'TnA_GC
Cuts at: 2697 2697
Size: 5632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2546 1186
Size: 1360 4272
BseYI C'CCAG_C
Cuts at: 4835 5331 4835
Size: 496 5136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5059
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3693 3778 4849 3693
Size: 85 1071 4476
Fragments arranged by size:
4476 1071 85
BsmI GAATG_Cn'
Cuts at: 2427 2689 3143 3236 2427
Size: 262 454 93 4823
Fragments arranged by size:
4823 454 262 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2951 3693 3778 4849 1391
Size: 399 1161 742 85 1071 2174
Fragments arranged by size:
2174 1161 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 2325 3521 5251 2325
Size: 1196 1730 2706
Fragments arranged by size:
2706 1730 1196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 844
Size: 2143 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2156 3519 1394
Size: 429 333 1363 3507
Fragments arranged by size:
3507 1363 429 333
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 2262 96
Size: 2166 3466
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2598 2962 3329 3714 3731 5183 5269 5281
Size: 626 364 367 385 17 1452 86 12
Cuts at: 5281 5627 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 626 385 367 364 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2558 2681 2558
Size: 123 5509
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3790
Fragments arranged by size:
3790 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 3079 3968
Size: 54 1425 50 275 18 546 703 889
Cuts at: 3968 5597 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 703 546 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2230 3562 1353
Size: 764 113 1332 3423
Fragments arranged by size:
3423 1332 764 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2486 4219 4593 4739 387
Size: 1064 138 897 1733 374 146 1280
Fragments arranged by size:
1733 1280 1064 897 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2400 2864 2927 3695 4292 5079 5347
Size: 393 768 464 63 768 597 787 268
Cuts at: 5347 1239
Size: 1524
Fragments arranged by size:
1524 787 768 768 597 464 393 268
63
EcoO109I rG'GnC_Cy
Cuts at: 2763 2763
Size: 5632
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
EcoRV GAT'ATC
Cuts at: 2331 2331
Size: 5632
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 4779 1678
Size: 3101 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373
Size: 32 135 32 2701 32 42 32 2626
Fragments arranged by size:
2701 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2527 2976 3222 669
Size: 988 281 589 449 246 3079
Fragments arranged by size:
3079 988 589 449 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 2017 3231 2017
Size: 1214 4418
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2541 4746 4930 1305
Size: 1236 2205 184 2007
Fragments arranged by size:
2205 2007 1236 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3264
Fragments arranged by size:
3264 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2324 2774 2852 3590 3949 4108
Size: 648 1011 147 450 78 738 359 159
Cuts at: 4108 518
Size: 2042
Fragments arranged by size:
2042 1011 738 648 450 359 159 147
78
MspA1I CmG'CkG
Cuts at: 1606 2696 3048 3725 4873 5118 1606
Size: 1090 352 677 1148 245 2120
Fragments arranged by size:
2120 1148 1090 677 352 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3790
Fragments arranged by size:
3790 939 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NsiI A_TGCA'T
Cuts at: 2293 2691 2293
Size: 398 5234
NspI r_CATG'y
Cuts at: 1132 1229 2295 2551 2662 2740 2990 4535 1132
Size: 97 1066 256 111 78 250 1545 2229
Fragments arranged by size:
2229 1545 1066 256 250 111 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 2658 4531 2658
Size: 1873 3759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2806 1108
Size: 1698 3934
PfoI T'CCnGG_A
Cuts at: 2416 2416
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2763 2763
Size: 5632
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2984 830
Size: 2154 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2696 2696
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SanDI GG'GwC_CC
Cuts at: 2763 2763
Size: 5632
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2455 2872 2980 4115 4796 4987 826
Size: 219 1410 417 108 1135 681 191 1471
Fragments arranged by size:
1471 1410 1135 681 417 219 191 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4637 4899 820
Size: 197 926 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 3071 513
Size: 552 939 351 716 3074
Fragments arranged by size:
3074 939 716 552 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2039 2254 2382 3659 3676 3829 4014
Size: 1255 459 215 128 1277 17 153 185
Cuts at: 4014 4459 325
Size: 445 1498
Fragments arranged by size:
1498 1277 1255 459 445 215 185 153
128 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2262 2778
Size: 274 80 33 51 1443 204 81 516
Cuts at: 2778 3878 5590 96
Size: 1100 1712 138
Fragments arranged by size:
1712 1443 1100 516 274 204 138 81
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 2714 3761 2714
Size: 1047 4585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI