Restriction map for
pFN21AE5388
(Circular) (Six-base) MAPSORT of: pFN21AE5388.seq Check: 723 from: 1 to: 5629
pFN21AE5388 5629 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3439 278
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2222 2972 1937
Size: 285 750 4594
Fragments arranged by size:
4594 750 285
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 2930 3692 5076 5344 1632
Size: 425 873 762 1384 268 1917
Fragments arranged by size:
1917 1384 873 762 425 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 2744 2843 1166
Size: 1578 99 3952
Fragments arranged by size:
3952 1578 99
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AlwNI CAG_nnn'CTG
Cuts at: 3372 4944 3372
Size: 1572 4057
ApaLI G'TGCA_C
Cuts at: 3686 4842 3686
Size: 1156 4473
ApoI r'AATT_y
Cuts at: 1493 2938 3164 3316 5425 1493
Size: 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 1870
Size: 73 1011 4545
Fragments arranged by size:
4545 1011 73
BamHI G'GATC_C
Cuts at: 2685 2959 3326 2685
Size: 274 367 4988
Fragments arranged by size:
4988 367 274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2950 4405 618
Size: 325 217 627 1163 1455 1842
Fragments arranged by size:
1842 1455 1163 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2447 2948 1391
Size: 1056 501 4072
Fragments arranged by size:
4072 1056 501
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3820 3854 1903
Size: 34 1883 34 3678
Fragments arranged by size:
3678 1883 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3521 4731 3521
Size: 1210 4419
BclI T'GATC_A
Cuts at: 1548 2814 1548
Size: 1266 4363
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4240 136
Size: 107 122 71 631 605 2568 1525
Fragments arranged by size:
2568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 3046 3046
Size: 5629
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2481 3690 4846 1790
Size: 451 240 1209 1156 2573
Fragments arranged by size:
2573 1209 1156 451 240
BmgBI CAC'GTC
Cuts at: 1318 2782 2830 1318
Size: 1464 48 4117
Fragments arranged by size:
4117 1464 48
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2057 2270 3360
Size: 416 53 83 399 24 640 213 1090
Cuts at: 3360 4318 442
Size: 958 1753
Fragments arranged by size:
1753 1090 958 640 416 399 213 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2835 4289 1239
Size: 992 604 1454 2579
Fragments arranged by size:
2579 1454 992 604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3754 4619 4917 3754
Size: 865 298 4466
Fragments arranged by size:
4466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4292 882
Size: 617 2793 2219
Fragments arranged by size:
2793 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2724 3420 493
Size: 711 1520 696 2702
Fragments arranged by size:
2702 1520 711 696
BsaBI GATnn'nnATC
Cuts at: 2108 5623 2108
Size: 3515 2114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3436 3818 4453 275
Size: 53 83 186 936 1903 382 635 1451
Fragments arranged by size:
1903 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4062 4460 4734 4881 5292 4062
Size: 398 274 147 411 4399
Fragments arranged by size:
4399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 4573
Fragments arranged by size:
4573 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2719 1186
Size: 1533 4096
BseYI C'CCAG_C
Cuts at: 4832 5328 4832
Size: 496 5133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2314 2554 1163
Size: 573 578 240 4238
Fragments arranged by size:
4238 578 573 240
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 3840 4868 1540
Size: 275 186 1078 761 1028 2301
Fragments arranged by size:
2301 1078 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2156 2729 2751 3690 3775 4846 2156
Size: 573 22 939 85 1071 2939
Fragments arranged by size:
2939 1071 939 573 85 22
BsmI GAATG_Cn'
Cuts at: 3140 3233 3140
Size: 93 5536
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2241 2447 2481 2729 2751 2948
Size: 399 366 85 206 34 248 22 197
Cuts at: 2948 3690 3775 4846 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 248 206 197
85 85 34 22
BspEI T'CCGG_A
Cuts at: 5292 5292
Size: 5629
BspHI T'CATG_A
Cuts at: 2409 3518 5248 2409
Size: 1109 1730 2790
Fragments arranged by size:
2790 1730 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2984 844
Size: 2140 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 1394
Size: 429 1693 3507
Fragments arranged by size:
3507 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2469 2544 4124 4298 65
Size: 1183 1221 75 1580 174 1396
Fragments arranged by size:
1580 1396 1221 1183 174 75
BsrFI r'CCGG_y
Cuts at: 1700 1810 4273 4460 1700
Size: 110 2463 187 2869
Fragments arranged by size:
2869 2463 187 110
BsrGI T'GTAC_A
Cuts at: 96 2106 2661 96
Size: 2010 555 3064
Fragments arranged by size:
3064 2010 555
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 3689 4701 3689
Size: 1012 4617
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5629
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2260 2248
Size: 12 5617
BstYI r'GATC_y
Cuts at: 1972 2685 2809 2959 3326 3711 3728 5180 5266
Size: 713 124 150 367 385 17 1452 86
Cuts at: 5266 5278 5624 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 713 385 367 346 150 124
86 17 12
BstZ17I GTA'TAC
Cuts at: 2223 2223
Size: 5629
Bsu36I CC'TnA_GG
Cuts at: 2681 2681
Size: 5629
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 2742 513
Size: 552 939 351 387 3400
Fragments arranged by size:
3400 939 552 387 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4890
Fragments arranged by size:
4890 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 2481 3145 3939 3959 2241
Size: 240 664 794 20 3911
Fragments arranged by size:
3911 794 664 240 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5594
DraI TTT'AAA
Cuts at: 2859 2933 2859
Size: 74 5555
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2466 2701
Size: 54 1425 50 275 18 420 216 235
Cuts at: 2701 3076 3965 5594 8
Size: 375 889 1629 43
Fragments arranged by size:
1629 1425 889 420 375 275 235 216
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 1537
Size: 275 1264 4090
Fragments arranged by size:
4090 1264 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 3559 1353
Size: 723 1483 3423
Fragments arranged by size:
3423 1483 723
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4216 4590 4736 387
Size: 1064 138 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2231 2835 2930 3692 4289 5076
Size: 393 425 174 604 95 762 597 787
Cuts at: 5076 5344 1239
Size: 268 1524
Fragments arranged by size:
1524 787 762 604 597 425 393 268
174 95
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FspI TGC'GCA
Cuts at: 4135 4135
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 4776 1678
Size: 3098 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4270 4302 4344 4376 1373
Size: 32 135 32 2698 32 42 32 2626
Fragments arranged by size:
2698 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2973 3219 669
Size: 988 281 1035 246 3079
Fragments arranged by size:
3079 1035 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4743 4927 1305
Size: 3438 184 2007
Fragments arranged by size:
3438 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 2468 10
Size: 54 1425 343 420 216 3171
Fragments arranged by size:
3171 1425 420 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 2744 2843 3587 3946 4105 518
Size: 648 1308 270 99 744 359 159 2042
Fragments arranged by size:
2042 1308 744 648 359 270 159 99
MspA1I CmG'CkG
Cuts at: 1606 2370 2434 3045 3722 4870 5115 1606
Size: 764 64 611 677 1148 245 2120
Fragments arranged by size:
2120 1148 764 677 611 245 64
NcoI C'CATG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3787
Fragments arranged by size:
3787 939 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2987 4532 1132
Size: 97 1758 1545 2229
Fragments arranged by size:
2229 1758 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2440 1348
Size: 1092 4537
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5629
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5629
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PmlI CAC'GTG
Cuts at: 2724 2724
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2370 1613
Size: 757 4872
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PstI C_TGCA'G
Cuts at: 830 2981 830
Size: 2151 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2434 2434
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 2339 3877 5589 2339
Size: 1538 1712 2379
Fragments arranged by size:
2379 1712 1538
SfcI C'TryA_G
Cuts at: 826 1045 2977 4112 4793 4984 826
Size: 219 1932 1135 681 191 1471
Fragments arranged by size:
1932 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3733 4634 4896 820
Size: 197 926 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5629
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 2805 3068 513
Size: 552 939 351 450 263 3074
Fragments arranged by size:
3074 939 552 450 351 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2440 3656 3673 3826 4011 4456 325
Size: 1255 860 1216 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1216 860 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2181 2307
Size: 274 80 33 51 1443 129 75 126
Cuts at: 2307 2337 2661 3875 5587 96
Size: 30 324 1214 1712 138
Fragments arranged by size:
1712 1443 1214 324 274 138 129 126
80 75 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2238 2270 5589 5621 2238
Size: 32 3319 32 2246
Fragments arranged by size:
3319 2246 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 3758 3758
Size: 5629
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3437 276
Size: 53 83 186 936 1903 2468
Fragments arranged by size:
2468 1903 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI