Restriction map for
pFN21AE5382
(Circular) (Six-base) MAPSORT of: pFN21AE5382.seq Check: 1674 from: 1 to: 5620
pFN21AE5382 5620 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2249 2963 1937
Size: 312 714 4594
Fragments arranged by size:
4594 714 312
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3683 5067 5335 1632
Size: 2051 1384 268 1917
Fragments arranged by size:
2051 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 2840 1166
Size: 1674 3946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AlwNI CAG_nnn'CTG
Cuts at: 2080 3363 4935 2080
Size: 1283 1572 2765
Fragments arranged by size:
2765 1572 1283
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2036 2929 3155 3307 5416 1493
Size: 543 893 226 152 2109 1697
Fragments arranged by size:
2109 1697 893 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2565 2598 2565
Size: 33 5587
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2055 2939 1391
Size: 664 884 4072
Fragments arranged by size:
4072 884 664
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2276 928
Size: 971 377 4272
Fragments arranged by size:
4272 971 377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 3681 4837 1790
Size: 1891 1156 2573
Fragments arranged by size:
2573 1891 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2608 4280 1239
Size: 818 551 1672 2579
Fragments arranged by size:
2579 1672 818 551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 4610 4908 3745
Size: 865 298 4457
Fragments arranged by size:
4457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2679 2848 4053 4451 4725 4872 5283 2679
Size: 169 1205 398 274 147 411 3016
Fragments arranged by size:
3016 1205 411 398 274 169 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5395
Fragments arranged by size:
5395 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5620
BseYI C'CCAG_C
Cuts at: 4823 5319 4823
Size: 496 5124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3681 3766 4837 3681
Size: 85 1071 4464
Fragments arranged by size:
4464 1071 85
BsmI GAATG_Cn'
Cuts at: 2711 3131 3224 2711
Size: 420 93 5107
Fragments arranged by size:
5107 420 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2939 3681 3766 4837 1391
Size: 399 265 884 742 85 1071 2174
Fragments arranged by size:
2174 1071 884 742 399 265 85
BspEI T'CCGG_A
Cuts at: 2679 5283 2679
Size: 2604 3016
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 844
Size: 2131 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2551 3507 1394
Size: 429 728 956 3507
Fragments arranged by size:
3507 956 728 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2313 2358 4115 4289 65
Size: 1183 1065 45 1757 174 1396
Fragments arranged by size:
1757 1396 1183 1065 174 45
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstXI CCAn_nnnn'nTGG
Cuts at: 2644 2644
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2510 2950 3317 3702 3719 5171 5257 5269
Size: 538 440 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 538 440 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5068
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 2029 3136 3930 3950 2029
Size: 1107 794 20 3699
Fragments arranged by size:
3699 1107 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 2433 3067
Size: 54 1425 50 275 18 543 60 634
Cuts at: 3067 3956 5585 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 634 543 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2477 3550 1353
Size: 1124 1073 3423
Fragments arranged by size:
3423 1124 1073
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2483 4207 4581 4727 387
Size: 1064 138 894 1724 374 146 1280
Fragments arranged by size:
1724 1280 1064 894 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2608 3683 4280 5067 5335 1239
Size: 393 425 551 1075 597 787 268 1524
Fragments arranged by size:
1524 1075 787 597 551 425 393 268
EcoRI G'AATT_C
Cuts at: 2036 2929 3307 2036
Size: 893 378 4349
Fragments arranged by size:
4349 893 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 5620
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2409 2441 2502 2534 2857
Size: 32 135 32 837 32 61 32 323
Cuts at: 2857 2889 4261 4293 4335 4367 1373
Size: 32 1372 32 42 32 2626
Fragments arranged by size:
2626 1372 837 323 135 61 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2250 2964 3210 669
Size: 988 281 312 714 246 3079
Fragments arranged by size:
3079 988 714 312 281 246
HindIII A'AGCT_T
Cuts at: 748 2558 748
Size: 1810 3810
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 4734 4918 1305
Size: 729 2700 184 2007
Fragments arranged by size:
2700 2007 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 2435 10
Size: 54 1425 343 543 60 3195
Fragments arranged by size:
3195 1425 543 343 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2141 2177 2840 3578 3937 4096 518
Size: 648 975 36 663 738 359 159 2042
Fragments arranged by size:
2042 975 738 663 648 359 159 36
MspA1I CmG'CkG
Cuts at: 1606 3036 3713 4861 5106 1606
Size: 1430 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5068
NdeI CA'TA_TG
Cuts at: 387 2733 387
Size: 2346 3274
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2344 2594 2776 2978 4523 1132
Size: 97 1115 250 182 202 1545 2229
Fragments arranged by size:
2229 1545 1115 250 202 182 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2737 1108
Size: 1629 3991
PfoI T'CCnGG_A
Cuts at: 2411 2411
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2972 830
Size: 2142 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2248 2962 1936
Size: 312 714 4594
Fragments arranged by size:
4594 714 312
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2395 2506 2869 2968 4103 4784 4975
Size: 219 1350 111 363 99 1135 681 191
Cuts at: 4975 826
Size: 1471
Fragments arranged by size:
1471 1350 1135 681 363 219 191 111
99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3724 4625 4887 820
Size: 197 926 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2594 2978 1132
Size: 1462 384 3774
Fragments arranged by size:
3774 1462 384
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 2900 1517
Size: 1383 4237
StyI C'CwwG_G
Cuts at: 513 1065 3059 513
Size: 552 1994 3074
Fragments arranged by size:
3074 1994 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3647 3664 3817 4002 4447 325
Size: 1255 2067 17 153 185 445 1498
Fragments arranged by size:
2067 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3866 5578
Size: 274 80 33 51 1443 204 1685 1712
Cuts at: 5578 96
Size: 138
Fragments arranged by size:
1712 1685 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI