Restriction map for
pFN21AE5360
(Circular) (Six-base) MAPSORT of: pFN21AE5360.seq Check: 6792 from: 1 to: 5485
pFN21AE5360 5485 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2619 2806 2619
Size: 187 5298
AclI AA'CG_TT
Cuts at: 1563 2784 3612 3985 1563
Size: 1221 828 373 3063
Fragments arranged by size:
3063 1221 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2426 2660 3548 4932 5200 1632
Size: 794 234 888 1384 268 1917
Fragments arranged by size:
1917 1384 888 794 268 234
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 4384 5361 1203
Size: 3181 977 1327
Fragments arranged by size:
3181 1327 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5485
AlwNI CAG_nnn'CTG
Cuts at: 2131 3228 4800 2131
Size: 1097 1572 2816
Fragments arranged by size:
2816 1572 1097
ApaI G_GGCC'C
Cuts at: 2149 2149
Size: 5485
ApaLI G'TGCA_C
Cuts at: 3542 4698 3542
Size: 1156 4329
ApoI r'AATT_y
Cuts at: 1493 2794 3020 3172 5281 1493
Size: 1301 226 152 2109 1697
Fragments arranged by size:
2109 1697 1301 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 2365 2810 1870
Size: 73 205 217 445 4545
Fragments arranged by size:
4545 445 217 205 73
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 2161 2355 2619 2806
Size: 325 217 627 365 9 194 264 187
Cuts at: 2806 4261 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 365 325 264 217 194
187 9
BanII G_rGCy'C
Cuts at: 1391 2149 2202 2479 2804 1391
Size: 758 53 277 325 4072
Fragments arranged by size:
4072 758 325 277 53
BbeI G_GCGC'C
Cuts at: 2156 2156
Size: 5485
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2475 2677 928
Size: 971 576 202 3736
Fragments arranged by size:
3736 971 576 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3676 3710 1903
Size: 34 1739 34 3678
Fragments arranged by size:
3678 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4096 136
Size: 107 122 71 631 605 2424 1525
Fragments arranged by size:
2424 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2043 2902 2043
Size: 859 4626
Bme1580I G_kGCm'C
Cuts at: 1790 2149 2166 2365 2414 3546 4702 1790
Size: 359 17 199 49 1132 1156 2573
Fragments arranged by size:
2573 1156 1132 359 199 49 17
BmgBI CAC'GTC
Cuts at: 1318 2548 1318
Size: 1230 4255
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2197 3216 4174
Size: 416 53 83 399 24 780 1019 958
Cuts at: 4174 442
Size: 1753
Fragments arranged by size:
1753 1019 958 780 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 4145 1239
Size: 841 2065 2579
Fragments arranged by size:
2579 2065 841
Bpu10I CC'TnA_GC
Cuts at: 2646 2646
Size: 5485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3610 4475 4773 3610
Size: 865 298 4322
Fragments arranged by size:
4322 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4148 882
Size: 617 2649 2219
Fragments arranged by size:
2649 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2153 3292 3674 4309
Size: 53 83 186 936 620 1139 382 635
Cuts at: 4309 275
Size: 1451
Fragments arranged by size:
1451 1139 936 635 620 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2304 2727 3918 4316 4590 4737 5148 2304
Size: 423 1191 398 274 147 411 2641
Fragments arranged by size:
2641 1191 423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5260
Fragments arranged by size:
5260 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2282 2629 1186
Size: 841 255 347 4042
Fragments arranged by size:
4042 841 347 255
BseYI C'CCAG_C
Cuts at: 2235 2255 2624 4688 5184 2235
Size: 20 369 2064 496 2536
Fragments arranged by size:
2536 2064 496 369 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 2366 1163
Size: 573 429 201 4282
Fragments arranged by size:
4282 573 429 201
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2380 2456 2935 3696 4724 1540
Size: 275 186 379 76 479 761 1028 2301
Fragments arranged by size:
2301 1028 761 479 379 275 186 76
BsiHKAI G_wGCw'C
Cuts at: 2202 3546 3631 4702 2202
Size: 1344 85 1071 2985
Fragments arranged by size:
2985 1344 1071 85
BsmI GAATG_Cn'
Cuts at: 2996 3089 2996
Size: 93 5392
BsmBI CGTCTCn'nnnn_
Cuts at: 2538 2538
Size: 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2149 2166 2202 2365 2414 2479 2804
Size: 399 359 17 36 163 49 65 325
Cuts at: 2804 3546 3631 4702 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 359 325 163 85
65 49 36 17
BspEI T'CCGG_A
Cuts at: 2304 5148 2304
Size: 2844 2641
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 2840 844
Size: 1343 653 3489
Fragments arranged by size:
3489 1343 653
BsrBI CCG'CTC
Cuts at: 1394 1823 2143 2458 3372 1394
Size: 429 320 315 914 3507
Fragments arranged by size:
3507 914 429 320 315
BsrDI GCAATG_nn'
Cuts at: 65 1248 3980 4154 65
Size: 1183 2732 174 1396
Fragments arranged by size:
2732 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2379 2400 4129 4316 1700
Size: 110 569 21 1729 187 2869
Fragments arranged by size:
2869 1729 569 187 110 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5485
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 2478 2747 3545 4557 2478
Size: 269 798 1012 3406
Fragments arranged by size:
3406 1012 798 269
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5485
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2263
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2569 2815 3182 3567 3584 5036 5122 5134
Size: 597 246 367 385 17 1452 86 12
Cuts at: 5134 5480 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 597 385 367 346 246 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2419 2435 513
Size: 552 939 415 16 3563
Fragments arranged by size:
3563 939 552 415 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4746
Fragments arranged by size:
4746 683 56
BtsI GCAGTG_nn'
Cuts at: 3001 3795 3815 3001
Size: 794 20 4671
Fragments arranged by size:
4671 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2789 2789
Size: 5485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2377 2453 2932
Size: 54 1425 50 275 18 547 76 479
Cuts at: 2932 3821 5450 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 547 479 275 76 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2377 2453 2932 1537
Size: 275 565 76 479 4090
Fragments arranged by size:
4090 565 479 275 76
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 3415 1353
Size: 1253 809 3423
Fragments arranged by size:
3423 1253 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4072 4446 4592 387
Size: 1064 138 2483 374 146 1280
Fragments arranged by size:
2483 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2200 2200
Size: 5485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2426 2660 3548 4145 4932 5200
Size: 393 448 346 234 888 597 787 268
Cuts at: 5200 1239
Size: 1524
Fragments arranged by size:
1524 888 787 597 448 393 346 268
234
EcoO109I rG'GnC_Cy
Cuts at: 2527 2527
Size: 5485
EcoRI G'AATT_C
Cuts at: 2794 3172 2794
Size: 378 5107
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 2156 2395 2515 4632 1678
Size: 478 239 120 2117 2531
Fragments arranged by size:
2531 2117 478 239 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2015 2035 2047 2442
Size: 32 135 32 431 12 20 12 395
Cuts at: 2442 2474 2561 2593 4126 4158 4200 4232 1373
Size: 32 87 32 1533 32 42 32 2626
Fragments arranged by size:
2626 1533 431 395 135 87 42 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 3075 669
Size: 988 281 891 246 3079
Fragments arranged by size:
3079 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5485
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KasI G'GCGC_C
Cuts at: 2152 2152
Size: 5485
KpnI G_GTAC'C
Cuts at: 2623 2810 2623
Size: 187 5298
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 4599 4783 1305
Size: 696 2598 184 2007
Fragments arranged by size:
2598 2007 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3663
Fragments arranged by size:
3663 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3443 3802 3961 518
Size: 648 2277 359 159 2042
Fragments arranged by size:
2277 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2488 2497 2592 2699 2901 3578 4726
Size: 441 441 9 95 107 202 677 1148
Cuts at: 4726 4971 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 441 441 245 202 107
95 9
NaeI GCC'GGC
Cuts at: 2381 2381
Size: 5485
NarI GG'CG_CC
Cuts at: 2153 2153
Size: 5485
NcoI C'CATG_G
Cuts at: 513 1065 2004 2419 513
Size: 552 939 415 3579
Fragments arranged by size:
3579 939 552 415
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NgoMIV G'CCGG_C
Cuts at: 2379 2379
Size: 5485
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2843 4388 1132
Size: 97 1614 1545 2229
Fragments arranged by size:
2229 1614 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5485
PciI A'CATG_T
Cuts at: 4384 4384
Size: 5485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2131 1108
Size: 1023 4462
PfoI T'CCnGG_A
Cuts at: 2058 2058
Size: 5485
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 2527
Size: 5485
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 3055 3055
Size: 5485
PspOMI G'GGCC_C
Cuts at: 2145 2145
Size: 5485
PstI C_TGCA'G
Cuts at: 830 2502 2837 830
Size: 1672 335 3478
Fragments arranged by size:
3478 1672 335
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
PvuII CAG'CTG
Cuts at: 2047 2497 2699 2047
Size: 450 202 4833
Fragments arranged by size:
4833 450 202
SacI G_AGCT'C
Cuts at: 2202 2202
Size: 5485
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SbfI CC_TGCA'GG
Cuts at: 2837 2837
Size: 5485
ScaI AGT'ACT
Cuts at: 3733 5445 3733
Size: 1712 3773
SfcI C'TryA_G
Cuts at: 826 1045 2498 2833 3968 4649 4840 826
Size: 219 1453 335 1135 681 191 1471
Fragments arranged by size:
1471 1453 1135 681 335 219 191
SfoI GGC'GCC
Cuts at: 2154 2154
Size: 5485
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2150 2812 1872
Size: 278 662 4545
Fragments arranged by size:
4545 662 278
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3589 4490 4752 820
Size: 197 926 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SrfI GCCC'GGGC
Cuts at: 2150 2150
Size: 5485
SspI AAT'ATT
Cuts at: 5 52 3409 5
Size: 47 3357 2081
Fragments arranged by size:
3357 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5485
StyI C'CwwG_G
Cuts at: 513 1065 2004 2419 2691 2924 513
Size: 552 939 415 272 233 3074
Fragments arranged by size:
3074 939 552 415 272 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3512 3529 3682 3867 4312 325
Size: 1255 1932 17 153 185 445 1498
Fragments arranged by size:
1932 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3731 5443 96
Size: 274 80 33 51 1443 1754 1712 138
Fragments arranged by size:
1754 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5445 5477 5445
Size: 32 5453
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2821 2821
Size: 5485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2728 2728
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2148 2810 1870
Size: 278 662 4545
Fragments arranged by size:
4545 662 278
XmnI GAAnn'nnTTC
Cuts at: 3614 3614
Size: 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SwaI