Restriction map for
pFN21AE5359
(Circular) (Six-base) MAPSORT of: pFN21AE5359.seq Check: 917 from: 1 to: 5626
pFN21AE5359 5626 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2153 2640 3436 278
Size: 53 83 186 936 617 487 796 2468
Fragments arranged by size:
2468 936 796 617 487 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2818 2947 2818
Size: 129 5497
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 3689 5073 5341 1632
Size: 602 1455 1384 268 1917
Fragments arranged by size:
1917 1455 1384 602 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 4525 5502 1203
Size: 3322 977 1327
Fragments arranged by size:
3322 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 2195 1166
Size: 1029 4597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5626
AlwNI CAG_nnn'CTG
Cuts at: 3369 4941 3369
Size: 1572 4054
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2935 3161 3313 5422 1493
Size: 1442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2727 4180 160
Size: 872 1695 1453 1606
Fragments arranged by size:
1695 1606 1453 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 1870
Size: 73 1008 4545
Fragments arranged by size:
4545 1008 73
BamHI G'GATC_C
Cuts at: 2840 2956 3323 2840
Size: 116 367 5143
Fragments arranged by size:
5143 367 116
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2818 2947 4402 618
Size: 325 217 627 1031 129 1455 1842
Fragments arranged by size:
1842 1455 1031 627 325 217 129
BanII G_rGCy'C
Cuts at: 1391 2945 1391
Size: 1554 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3817 3851 1903
Size: 34 1880 34 3678
Fragments arranged by size:
3678 1880 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 2091 1548
Size: 543 5083
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2245 4237 136
Size: 107 122 71 631 605 573 1992 1525
Fragments arranged by size:
1992 1525 631 605 573 122 107 71
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 2760 3687 4843 1790
Size: 970 927 1156 2573
Fragments arranged by size:
2573 1156 970 927
BmgBI CAC'GTC
Cuts at: 1318 2089 1318
Size: 771 4855
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2394 4286 1239
Size: 872 283 1892 2579
Fragments arranged by size:
2579 1892 872 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3751 4616 4914 3751
Size: 865 298 4463
Fragments arranged by size:
4463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3417 493
Size: 711 2213 2702
Fragments arranged by size:
2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2150 2637 3433 3815
Size: 53 83 186 936 617 487 796 382
Cuts at: 3815 4450 275
Size: 635 1451
Fragments arranged by size:
1451 936 796 635 617 487 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2370 2400 1178
Size: 30 165 30 967 30 4404
Fragments arranged by size:
4404 967 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2391 1186
Size: 1205 4421
BseYI C'CCAG_C
Cuts at: 4829 5325 4829
Size: 496 5130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5053
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3076 3837 4865 1540
Size: 275 186 1075 761 1028 2301
Fragments arranged by size:
2301 1075 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3687 3772 4843 3687
Size: 85 1071 4470
Fragments arranged by size:
4470 1071 85
BsmI GAATG_Cn'
Cuts at: 2147 3137 3230 2147
Size: 990 93 4543
Fragments arranged by size:
4543 990 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2760 2945 3687 3772 4843 1391
Size: 399 970 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 970 742 399 185 85
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 3515 5245 3515
Size: 1730 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 844
Size: 2137 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2775 4121 4295 65
Size: 1183 1527 1346 174 1396
Fragments arranged by size:
1527 1396 1346 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4270 4457 1700
Size: 110 2460 187 2869
Fragments arranged by size:
2869 2460 187 110
BsrGI T'GTAC_A
Cuts at: 96 2570 96
Size: 2474 3152
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5626
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2840 2956 3323 3708 3725 5177 5263 5275
Size: 868 116 367 385 17 1452 86 12
Cuts at: 5275 5621 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 868 385 367 346 116 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2446 513
Size: 552 939 442 3693
Fragments arranged by size:
3693 939 552 442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3936 3956 3142
Size: 794 20 4812
Fragments arranged by size:
4812 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DraIII CAC_nnn'GTG
Cuts at: 2653 2653
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 3073 3962
Size: 54 1425 50 275 18 282 961 889
Cuts at: 3962 5591 8
Size: 1629 43
Fragments arranged by size:
1629 1425 961 889 282 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3073 1537
Size: 275 1261 4090
Fragments arranged by size:
4090 1261 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 3556 1353
Size: 707 1496 3423
Fragments arranged by size:
3423 1496 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 2230 2257 4213 4587 4733
Size: 1064 138 510 131 27 1956 374 146
Cuts at: 4733 387
Size: 1280
Fragments arranged by size:
1956 1280 1064 510 374 146 138 131
27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2234 2394 3689 4286 5073 5341
Size: 393 479 123 160 1295 597 787 268
Cuts at: 5341 1239
Size: 1524
Fragments arranged by size:
1524 1295 787 597 479 393 268 160
123
EcoNI CCTnn'n_nnAGG
Cuts at: 2275 2340 2275
Size: 65 5561
EcoO109I rG'GnC_Cy
Cuts at: 2011 2011
Size: 5626
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2441 2623 2441
Size: 182 5444
FspI TGC'GCA
Cuts at: 2680 4132 2680
Size: 1452 4174
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373
Size: 32 135 32 2695 32 42 32 2626
Fragments arranged by size:
2695 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2822 2951 2822
Size: 129 5497
MfeI C'AATT_G
Cuts at: 2566 3225 2566
Size: 659 4967
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4740 4924 1305
Size: 3435 184 2007
Fragments arranged by size:
3435 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3804
Fragments arranged by size:
3804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 3584 3943 4102 518
Size: 648 1029 1389 359 159 2042
Fragments arranged by size:
2042 1389 1029 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2104 2195 2232 2254 3042 3719 4867
Size: 457 41 91 37 22 788 677 1148
Cuts at: 4867 5112 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 788 677 457 245 91 41
37 22
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4135
Fragments arranged by size:
4135 939 552
NdeI CA'TA_TG
Cuts at: 387 2587 387
Size: 2200 3426
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2984 4529 1132
Size: 97 1755 1545 2229
Fragments arranged by size:
2229 1755 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2041 1348
Size: 693 4933
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5626
PfoI T'CCnGG_A
Cuts at: 2301 2843 2301
Size: 542 5084
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 2478 3196 2478
Size: 718 4908
PstI C_TGCA'G
Cuts at: 830 2978 830
Size: 2148 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
PvuII CAG'CTG
Cuts at: 2063 2063
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2060
Size: 5626
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2227 2974 4109 4790 4981 826
Size: 219 1182 747 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 747 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3730 4631 4893 820
Size: 197 926 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 2606 1517
Size: 1089 4537
StyI C'CwwG_G
Cuts at: 513 1065 2004 2134 3065 513
Size: 552 939 130 931 3074
Fragments arranged by size:
3074 939 931 552 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3653 3670 3823 4008 4453 325
Size: 1255 2073 17 153 185 445 1498
Fragments arranged by size:
2073 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2570 2745 3872
Size: 274 80 33 51 1443 593 175 1127
Cuts at: 3872 5584 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1127 593 274 175 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 2297 1316
Size: 981 4645
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 2777 3755 2777
Size: 978 4648
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2151 2638 3434 276
Size: 53 83 186 936 617 487 796 2468
Fragments arranged by size:
2468 936 796 617 487 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI