Restriction map for
pFN21AE5358
(Circular) (Six-base) MAPSORT of: pFN21AE5358.seq Check: 5343 from: 1 to: 5626
pFN21AE5358 5626 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 2969 1937
Size: 883 149 4594
Fragments arranged by size:
4594 883 149
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2390 2723 2842 3689 5073 5341 1632
Size: 758 333 119 847 1384 268 1917
Fragments arranged by size:
1917 1384 847 758 333 268 119
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 4525 5502 1203
Size: 3322 977 1327
Fragments arranged by size:
3322 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2144 1184
Size: 960 4666
AlwNI CAG_nnn'CTG
Cuts at: 2896 3369 4941 2896
Size: 473 1572 3581
Fragments arranged by size:
3581 1572 473
ApaLI G'TGCA_C
Cuts at: 2195 3683 4839 2195
Size: 1488 1156 2982
Fragments arranged by size:
2982 1488 1156
ApoI r'AATT_y
Cuts at: 1493 2477 2492 2771 2935 3161 3313 5422 1493
Size: 984 15 279 164 226 152 2109 1697
Fragments arranged by size:
2109 1697 984 279 226 164 152 15
AseI AT'TA_AT
Cuts at: 160 1032 2616 4180 160
Size: 872 1584 1564 1606
Fragments arranged by size:
1606 1584 1564 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2951 1870
Size: 73 1008 4545
Fragments arranged by size:
4545 1008 73
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2392 2947 4402 618
Size: 325 217 627 372 233 555 1455 1842
Fragments arranged by size:
1842 1455 627 555 372 325 233 217
BanII G_rGCy'C
Cuts at: 1391 2414 2701 2945 1391
Size: 1023 287 244 4072
Fragments arranged by size:
4072 1023 287 244
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 928
Size: 971 149 4506
Fragments arranged by size:
4506 971 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3817 3851 1903
Size: 34 50 34 1796 34 3678
Fragments arranged by size:
3678 1796 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 2754 1548
Size: 1206 4420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4237 136
Size: 107 122 71 631 605 2565 1525
Fragments arranged by size:
2565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5621 5621
Size: 5626
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 2199 2397 2661 3687 4843 1790
Size: 409 198 264 1026 1156 2573
Fragments arranged by size:
2573 1156 1026 409 264 198
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2652 2652
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4286 1239
Size: 3047 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2852 3751 4616 4914 2852
Size: 899 865 298 3564
Fragments arranged by size:
3564 899 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2246 2602 4289 882
Size: 617 747 356 1687 2219
Fragments arranged by size:
2219 1687 747 617 356
BsaAI yAC'GTr
Cuts at: 493 1204 3417 493
Size: 711 2213 2702
Fragments arranged by size:
2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3433 3815 4450 275
Size: 53 83 186 936 1900 382 635 1451
Fragments arranged by size:
1900 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4059 4457 4731 4878 5289 4059
Size: 398 274 147 411 4396
Fragments arranged by size:
4396 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5401
Fragments arranged by size:
5401 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2641 1186
Size: 1455 4171
BseYI C'CCAG_C
Cuts at: 2095 2675 4829 5325 2095
Size: 580 2154 496 2396
Fragments arranged by size:
2396 2154 580 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5053
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2248 3076 3837 4865 1540
Size: 275 186 247 828 761 1028 2301
Fragments arranged by size:
2301 1028 828 761 275 247 186
BsiHKAI G_wGCw'C
Cuts at: 2199 2701 3687 3772 4843 2199
Size: 502 986 85 1071 2982
Fragments arranged by size:
2982 1071 986 502 85
BsmI GAATG_Cn'
Cuts at: 2649 3137 3230 2649
Size: 488 93 5045
Fragments arranged by size:
5045 488 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2397 2414 2661 2701 2945 3687
Size: 399 409 198 17 247 40 244 742
Cuts at: 3687 3772 4843 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 409 399 247 244 198
85 40 17
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 3515 5245 3515
Size: 1730 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2981 844
Size: 1268 869 3489
Fragments arranged by size:
3489 1268 869
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 1394
Size: 429 1690 3507
Fragments arranged by size:
3507 1690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4121 4295 65
Size: 1183 2873 174 1396
Fragments arranged by size:
2873 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4270 4457 1700
Size: 110 2460 187 2869
Fragments arranged by size:
2869 2460 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 3686 4698 3686
Size: 1012 4614
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2665 794
Size: 1871 3755
BstBI TT'CG_AA
Cuts at: 2875 4430 2875
Size: 1555 4071
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2956 3323 3708 3725 5177 5263 5275 5621
Size: 984 367 385 17 1452 86 12 346
Cuts at: 5621 1972
Size: 1977
Fragments arranged by size:
1977 1452 984 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2804 513
Size: 552 1739 3335
Fragments arranged by size:
3335 1739 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3936 3956 3142
Size: 794 20 4812
Fragments arranged by size:
4812 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5591
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2245 2801 2922
Size: 54 1425 50 275 18 415 556 121
Cuts at: 2922 3073 3962 5591 8
Size: 151 889 1629 43
Fragments arranged by size:
1629 1425 889 556 415 275 151 121
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2245 3073 1537
Size: 275 433 828 4090
Fragments arranged by size:
4090 828 433 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3556 1353
Size: 2203 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2699 2699
Size: 5626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2723 2842 3689 4286 5073 5341
Size: 393 758 333 119 847 597 787 268
Cuts at: 5341 1239
Size: 1524
Fragments arranged by size:
1524 847 787 758 597 393 333 268
119
EcoO109I rG'GnC_Cy
Cuts at: 2671 2671
Size: 5626
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FspI TGC'GCA
Cuts at: 4132 4132
Size: 5626
HaeII r_GCGC'y
Cuts at: 1678 4773 1678
Size: 3095 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373
Size: 32 135 32 2695 32 42 32 2626
Fragments arranged by size:
2695 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2870 748
Size: 2122 3504
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4740 4924 1305
Size: 3435 184 2007
Fragments arranged by size:
3435 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2803 10
Size: 54 1425 343 971 2833
Fragments arranged by size:
2833 1425 971 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3584 3943 4102 518
Size: 648 2418 359 159 2042
Fragments arranged by size:
2418 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2307 3042 3719 4867 5112 1606
Size: 701 735 677 1148 245 2120
Fragments arranged by size:
2120 1148 735 701 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2804 513
Size: 552 1739 3335
Fragments arranged by size:
3335 1739 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2984 4529 1132
Size: 97 1755 1545 2229
Fragments arranged by size:
2229 1755 1545 97
PacI TTA_AT'TAA
Cuts at: 2616 2616
Size: 5626
PasI CC'CwG_GG
Cuts at: 1348 2384 1348
Size: 1036 4590
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2295 1108
Size: 1187 4439
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2671 2671
Size: 5626
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2495 2527 2780 2812 2495
Size: 32 253 32 5309
Fragments arranged by size:
5309 253 32 32
PstI C_TGCA'G
Cuts at: 830 2181 2670 2978 830
Size: 1351 489 308 3478
Fragments arranged by size:
3478 1351 489 308
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
PvuII CAG'CTG
Cuts at: 2307 2307
Size: 5626
SacI G_AGCT'C
Cuts at: 2701 2701
Size: 5626
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2670 2978 2670
Size: 308 5318
ScaI AGT'ACT
Cuts at: 2231 3874 5586 2231
Size: 1643 1712 2271
Fragments arranged by size:
2271 1712 1643
SfcI C'TryA_G
Cuts at: 826 1045 2177 2666 2974 4109 4790 4981 826
Size: 219 1132 489 308 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 681 489 308 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5626
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2867 3730 4631 4893 820
Size: 197 926 924 863 901 262 1553
Fragments arranged by size:
1553 926 924 901 863 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5626
StyI C'CwwG_G
Cuts at: 513 1065 2133 2804 3065 513
Size: 552 1068 671 261 3074
Fragments arranged by size:
3074 1068 671 552 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2147 2380 2443 2696 2908 3653 3670
Size: 1255 567 233 63 253 212 745 17
Cuts at: 3670 3823 4008 4453 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 745 567 445 253 233 212
185 153 63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 3872 5584
Size: 274 80 33 51 1443 252 1643 1712
Cuts at: 5584 96
Size: 138
Fragments arranged by size:
1712 1643 1443 274 252 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 2078 2494 3755 2078
Size: 416 1261 3949
Fragments arranged by size:
3949 1261 416
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PacI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PfoI PmlI PspOMI PspXI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI