Restriction map for
pFN21AE5355
(Circular) (Six-base) MAPSORT of: pFN21AE5355.seq Check: 3083 from: 1 to: 5587
pFN21AE5355 5587 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3397 278
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2930 1937
Size: 993 4594
Acc65I G'GTAC_C
Cuts at: 2675 2908 2675
Size: 233 5354
AclI AA'CG_TT
Cuts at: 1563 3714 4087 1563
Size: 2151 373 3063
Fragments arranged by size:
3063 2151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2634 3650 5034 5302 1632
Size: 1002 1016 1384 268 1917
Fragments arranged by size:
1917 1384 1016 1002 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5390
AflIII A'CryG_T
Cuts at: 1203 4486 5463 1203
Size: 3283 977 1327
Fragments arranged by size:
3283 1327 977
AgeI A'CCGG_T
Cuts at: 4418 4418
Size: 5587
AhdI GACnn_n'nnGTC
Cuts at: 4316 4316
Size: 5587
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5587
AlwNI CAG_nnn'CTG
Cuts at: 2189 2788 3330 4902 2189
Size: 599 542 1572 2874
Fragments arranged by size:
2874 1572 599 542
ApaLI G'TGCA_C
Cuts at: 3644 4800 3644
Size: 1156 4431
ApoI r'AATT_y
Cuts at: 1493 2156 2603 2896 3122 3274 5383 1493
Size: 663 447 293 226 152 2109 1697
Fragments arranged by size:
2109 1697 663 447 293 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2841 4141 160
Size: 872 1809 1300 1606
Fragments arranged by size:
1809 1606 1300 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
AvaI C'yCGr_G
Cuts at: 1870 1943 2912 1870
Size: 73 969 4545
Fragments arranged by size:
4545 969 73
BamHI G'GATC_C
Cuts at: 2719 2917 3284 2719
Size: 198 367 5022
Fragments arranged by size:
5022 367 198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 2908 4363 618
Size: 325 217 627 888 233 1455 1842
Fragments arranged by size:
1842 1455 888 627 325 233 217
BanII G_rGCy'C
Cuts at: 1391 2868 2906 1391
Size: 1477 38 4072
Fragments arranged by size:
4072 1477 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2307 2376 928
Size: 971 408 69 4139
Fragments arranged by size:
4139 971 408 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3778 3812 1903
Size: 34 1841 34 3678
Fragments arranged by size:
3678 1841 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3479 4689 3479
Size: 1210 4377
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5587
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4198 136
Size: 107 122 71 631 605 2526 1525
Fragments arranged by size:
2526 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2638 5582 2638
Size: 2944 2643
BlpI GC'TnA_GC
Cuts at: 3004 3004
Size: 5587
Bme1580I G_kGCm'C
Cuts at: 1790 3648 4804 1790
Size: 1858 1156 2573
Fragments arranged by size:
2573 1858 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2211 2571 3318
Size: 416 53 83 399 24 794 360 747
Cuts at: 3318 4276 442
Size: 958 1753
Fragments arranged by size:
1753 958 794 747 416 399 360 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2868 2894 4247 1239
Size: 1629 26 1353 2579
Fragments arranged by size:
2579 1629 1353 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3712 4577 4875 3712
Size: 865 298 4424
Fragments arranged by size:
4424 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2207 4250 882
Size: 617 708 2043 2219
Fragments arranged by size:
2219 2043 708 617
BsaAI yAC'GTr
Cuts at: 493 1204 3378 493
Size: 711 2174 2702
Fragments arranged by size:
2702 2174 711
BsaBI GATnn'nnATC
Cuts at: 5581 5581
Size: 5587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3394 3776 4411 275
Size: 53 83 186 936 1861 382 635 1451
Fragments arranged by size:
1861 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2168 4020 4418 4692 4839 5250 2168
Size: 1852 398 274 147 411 2505
Fragments arranged by size:
2505 1852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5362
Fragments arranged by size:
5362 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5587
BseYI C'CCAG_C
Cuts at: 4790 5286 4790
Size: 496 5091
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2668 1163
Size: 573 932 4082
Fragments arranged by size:
4082 932 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3798 4826 1540
Size: 275 186 1036 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3648 3733 4804 3648
Size: 85 1071 4431
Fragments arranged by size:
4431 1071 85
BsmI GAATG_Cn'
Cuts at: 3098 3191 3098
Size: 93 5494
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2868 2906 3648 3733 4804 1391
Size: 399 1078 38 742 85 1071 2174
Fragments arranged by size:
2174 1078 1071 742 399 85 38
BspEI T'CCGG_A
Cuts at: 5250 5250
Size: 5587
BspHI T'CATG_A
Cuts at: 3476 5206 3476
Size: 1730 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2452 2942 844
Size: 1608 490 3489
Fragments arranged by size:
3489 1608 490
BsrBI CCG'CTC
Cuts at: 1394 1823 3474 1394
Size: 429 1651 3507
Fragments arranged by size:
3507 1651 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2697 4082 4256 65
Size: 1183 1449 1385 174 1396
Fragments arranged by size:
1449 1396 1385 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4231 4418 1700
Size: 110 2421 187 2869
Fragments arranged by size:
2869 2421 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5587
BssHII G'CGCG_C
Cuts at: 1925 2028 1925
Size: 103 5484
BssSI C'ACGA_G
Cuts at: 3647 4659 3647
Size: 1012 4575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5587
BstBI TT'CG_AA
Cuts at: 4391 4391
Size: 5587
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5587
BstXI CCAn_nnnn'nTGG
Cuts at: 2861 2861
Size: 5587
BstYI r'GATC_y
Cuts at: 1972 2638 2719 2917 3284 3669 3686 5138 5224
Size: 666 81 198 367 385 17 1452 86
Cuts at: 5224 5236 5582 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 666 385 367 346 198 86
81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2014 2452 513
Size: 552 939 10 438 3648
Fragments arranged by size:
3648 939 552 438 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4848
Fragments arranged by size:
4848 683 56
BtsI GCAGTG_nn'
Cuts at: 2152 2553 2724 3103 3897 3917 2152
Size: 401 171 379 794 20 3822
Fragments arranged by size:
3822 794 401 379 171 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5552
DraI TTT'AAA
Cuts at: 2891 2891
Size: 5587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4594 809
Size: 507 3278 1802
Fragments arranged by size:
3278 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3034 3923 5552
Size: 54 1425 50 275 18 1204 889 1629
Cuts at: 5552 8
Size: 43
Fragments arranged by size:
1629 1425 1204 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3034 1537
Size: 275 1222 4090
Fragments arranged by size:
4090 1222 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2828 3517 1353
Size: 1475 689 3423
Fragments arranged by size:
3423 1475 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4174 4548 4694 387
Size: 1064 138 2585 374 146 1280
Fragments arranged by size:
2585 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2634 2868 2894 3650 4247 5034 5302
Size: 393 1002 234 26 756 597 787 268
Cuts at: 5302 1239
Size: 1524
Fragments arranged by size:
1524 1002 787 756 597 393 268 234
26
EcoO109I rG'GnC_Cy
Cuts at: 2205 2205
Size: 5587
EcoRI G'AATT_C
Cuts at: 2896 3274 2896
Size: 378 5209
FspI TGC'GCA
Cuts at: 4093 4093
Size: 5587
HaeII r_GCGC'y
Cuts at: 1678 4734 1678
Size: 3056 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2433 2465 4228 4260 4302
Size: 32 135 32 861 32 1763 32 42
Cuts at: 4302 4334 1373
Size: 32 2626
Fragments arranged by size:
2626 1763 861 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 2749 2931 3177 669
Size: 988 281 487 324 182 246 3079
Fragments arranged by size:
3079 988 487 324 281 246 182
HindIII A'AGCT_T
Cuts at: 748 2804 748
Size: 2056 3531
HpaI GTT'AAC
Cuts at: 3177 3177
Size: 5587
KpnI G_GTAC'C
Cuts at: 2679 2912 2679
Size: 233 5354
MfeI C'AATT_G
Cuts at: 2295 3186 2295
Size: 891 4696
MluI A'CGCG_T
Cuts at: 5463 5463
Size: 5587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2697 2859 4701 4885 1305
Size: 1392 162 1842 184 2007
Fragments arranged by size:
2007 1842 1392 184 162
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3765
Fragments arranged by size:
3765 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2583 3545 3904 4063 518
Size: 648 1417 962 359 159 2042
Fragments arranged by size:
2042 1417 962 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2462 3003 3680 4828 5073 1606
Size: 856 541 677 1148 245 2120
Fragments arranged by size:
2120 1148 856 677 541 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2452 513
Size: 552 939 448 3648
Fragments arranged by size:
3648 939 552 448
NdeI CA'TA_TG
Cuts at: 387 2056 387
Size: 1669 3918
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5587
NotI GC'GGCC_GC
Cuts at: 3034 3034
Size: 5587
NspI r_CATG'y
Cuts at: 1132 1229 2945 4490 1132
Size: 97 1716 1545 2229
Fragments arranged by size:
2229 1716 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5587
PciI A'CATG_T
Cuts at: 4486 4486
Size: 5587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5587
PmeI GTTT'AAAC
Cuts at: 2891 2891
Size: 5587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 5169 5201 3656
Size: 32 1481 32 4042
Fragments arranged by size:
4042 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2205 2205
Size: 5587
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5587
PsiI TTA'TAA
Cuts at: 3157 3157
Size: 5587
PstI C_TGCA'G
Cuts at: 830 2939 830
Size: 2109 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5587
PvuII CAG'CTG
Cuts at: 2462 2462
Size: 5587
SalI G'TCGA_C
Cuts at: 1936 2929 1936
Size: 993 4594
SapI GCTCTTCn'nnn_
Cuts at: 2828 2828
Size: 5587
SbfI CC_TGCA'GG
Cuts at: 2939 2939
Size: 5587
ScaI AGT'ACT
Cuts at: 3835 5547 3835
Size: 1712 3875
SfcI C'TryA_G
Cuts at: 826 1045 2554 2935 4070 4751 4942 826
Size: 219 1509 381 1135 681 191 1471
Fragments arranged by size:
1509 1471 1135 681 381 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5587
SmaI CCC'GGG
Cuts at: 1872 2914 1872
Size: 1042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3691 4592 4854 820
Size: 197 926 1748 901 262 1553
Fragments arranged by size:
1748 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3378 493
Size: 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5587
SphI G_CATG'C
Cuts at: 1132 2945 1132
Size: 1813 3774
SspI AAT'ATT
Cuts at: 5 52 3511 5
Size: 47 3459 2081
Fragments arranged by size:
3459 2081 47
StuI AGG'CCT
Cuts at: 1517 2794 1517
Size: 1277 4310
StyI C'CwwG_G
Cuts at: 513 1065 2004 2065 2208 2410 2452 3026 513
Size: 552 939 61 143 202 42 574 3074
Fragments arranged by size:
3074 939 574 552 202 143 61 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3614 3631 3784 3969 4414 325
Size: 1255 2034 17 153 185 445 1498
Fragments arranged by size:
2034 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3833 5545 96
Size: 274 80 33 51 1443 1856 1712 138
Fragments arranged by size:
1856 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5547 5579 5547
Size: 32 5555
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5587
XbaI T'CTAG_A
Cuts at: 2923 2923
Size: 5587
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5587
XmaI C'CCGG_G
Cuts at: 1870 2912 1870
Size: 1042 4545
XmnI GAAnn'nnTTC
Cuts at: 3716 3716
Size: 5587
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3395 276
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI