Restriction map for
pFN21AE5353
(Circular) (Six-base) MAPSORT of: pFN21AE5353.seq Check: 4969 from: 1 to: 5680
pFN21AE5353 5680 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2602 2838 2602
Size: 236 5444
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 2845 3001 2845
Size: 156 5524
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 2486 2624 3743 5127 5395 1632
Size: 617 237 138 1119 1384 268 1917
Fragments arranged by size:
1917 1384 1119 617 268 237 138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 2858 3423 4995 2858
Size: 565 1572 3543
Fragments arranged by size:
3543 1572 565
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2104 2564 2731 2989 3215 3367 5476 1493
Size: 611 460 167 258 226 152 2109 1697
Fragments arranged by size:
2109 1697 611 460 258 226 167 152
AseI AT'TA_AT
Cuts at: 160 1032 2697 4234 160
Size: 872 1665 1537 1606
Fragments arranged by size:
1665 1606 1537 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 2556 3005 1870
Size: 73 400 213 449 4545
Fragments arranged by size:
4545 449 400 213 73
BamHI G'GATC_C
Cuts at: 2278 3010 3377 2278
Size: 732 367 4581
Fragments arranged by size:
4581 732 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2845 3001 4456 618
Size: 325 217 627 1058 156 1455 1842
Fragments arranged by size:
1842 1455 1058 627 325 217 156
BanII G_rGCy'C
Cuts at: 1391 2154 2999 1391
Size: 763 845 4072
Fragments arranged by size:
4072 845 763
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2237 2637 928
Size: 971 338 400 3971
Fragments arranged by size:
3971 971 400 338
BbvCI CC'TCA_GC
Cuts at: 2261 2261
Size: 5680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2027 3741 4897 1790
Size: 237 1714 1156 2573
Fragments arranged by size:
2573 1714 1156 237
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3411 4369
Size: 416 53 83 399 24 1293 701 958
Cuts at: 4369 442
Size: 1753
Fragments arranged by size:
1753 1293 958 701 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2571 2571
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2880 4340 1239
Size: 1641 1460 2579
Fragments arranged by size:
2579 1641 1460
Bpu10I CC'TnA_GC
Cuts at: 2261 2261
Size: 5680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 2283 5674 2283
Size: 3391 2289
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2244 3487 3869 4504
Size: 53 83 186 936 711 1243 382 635
Cuts at: 4504 275
Size: 1451
Fragments arranged by size:
1451 1243 936 711 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2977 4113 4511 4785 4932 5343 2977
Size: 1136 398 274 147 411 3314
Fragments arranged by size:
3314 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5455
Fragments arranged by size:
5455 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 2591 2696 1186
Size: 1192 213 105 4170
Fragments arranged by size:
4170 1192 213 105
BseYI C'CCAG_C
Cuts at: 4883 5379 4883
Size: 496 5184
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2888 1163
Size: 573 1152 3955
Fragments arranged by size:
3955 1152 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2651 3130 3891 4919 1540
Size: 275 186 650 479 761 1028 2301
Fragments arranged by size:
2301 1028 761 650 479 275 186
BsiHKAI G_wGCw'C
Cuts at: 2154 2886 3741 3826 4897 2154
Size: 732 855 85 1071 2937
Fragments arranged by size:
2937 1071 855 732 85
BsmI GAATG_Cn'
Cuts at: 3191 3284 3191
Size: 93 5587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2154 2886 2999 3741 3826 4897
Size: 399 237 127 732 113 742 85 1071
Cuts at: 4897 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 732 399 237 127 113
85
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2602 2838 3035 844
Size: 1758 236 197 3489
Fragments arranged by size:
3489 1758 236 197
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2177 4175 4349 65
Size: 1183 929 1998 174 1396
Fragments arranged by size:
1998 1396 1183 929 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2349 4324 4511 1700
Size: 110 539 1975 187 2869
Fragments arranged by size:
2869 1975 539 187 110
BsrGI T'GTAC_A
Cuts at: 96 2017 2932 96
Size: 1921 915 2844
Fragments arranged by size:
2844 1921 915
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2614 794
Size: 1820 3860
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2278 2836 3010 3377 3762 3779 5231 5317
Size: 306 558 174 367 385 17 1452 86
Cuts at: 5317 5329 5675 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 558 385 367 346 306 174
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2247 2840 513
Size: 552 939 243 593 3353
Fragments arranged by size:
3353 939 593 552 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2315 487
Size: 683 56 1089 3852
Fragments arranged by size:
3852 1089 683 56
BtsI GCAGTG_nn'
Cuts at: 3196 3990 4010 3196
Size: 794 20 4866
Fragments arranged by size:
4866 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2427 2984 2427
Size: 557 5123
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3127 4016 5645
Size: 54 1425 50 275 18 1297 889 1629
Cuts at: 5645 8
Size: 43
Fragments arranged by size:
1629 1425 1297 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 2505 3610 1353
Size: 1053 99 1105 3423
Fragments arranged by size:
3423 1105 1053 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2913 4267 4641 4787 387
Size: 1064 138 1324 1354 374 146 1280
Fragments arranged by size:
1354 1324 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2152 2152
Size: 5680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2486 2624 2880 3743 4340 5127
Size: 393 617 237 138 256 863 597 787
Cuts at: 5127 5395 1239
Size: 268 1524
Fragments arranged by size:
1524 863 787 617 597 393 268 256
237 138
EcoNI CCTnn'n_nnAGG
Cuts at: 2492 2492
Size: 5680
EcoO109I rG'GnC_Cy
Cuts at: 2855 2855
Size: 5680
EcoRI G'AATT_C
Cuts at: 2104 2564 2731 2989 3367 2104
Size: 460 167 258 378 4417
Fragments arranged by size:
4417 460 378 258 167
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 4827 1678
Size: 3149 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 2653 2685 4321
Size: 32 135 32 520 32 529 32 1636
Cuts at: 4321 4353 4395 4427 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1636 529 520 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 3024 3270 669
Size: 988 281 226 860 246 3079
Fragments arranged by size:
3079 988 860 281 246 226
HindIII A'AGCT_T
Cuts at: 748 2745 748
Size: 1997 3683
HpaI GTT'AAC
Cuts at: 2164 3270 2164
Size: 1106 4574
KpnI G_GTAC'C
Cuts at: 2849 3005 2849
Size: 156 5524
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3858
Fragments arranged by size:
3858 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3638 3997 4156 518
Size: 648 2472 359 159 2042
Fragments arranged by size:
2472 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2249 2359 3096 3773 4921 5166 1606
Size: 643 110 737 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 677 643 245 110
NcoI C'CATG_G
Cuts at: 513 1065 2004 2840 513
Size: 552 939 836 3353
Fragments arranged by size:
3353 939 836 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NruI TCG'CGA
Cuts at: 2334 2334
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 3038 4583 1132
Size: 97 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PfoI T'CCnGG_A
Cuts at: 2154 2154
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2358 3032 830
Size: 1528 674 3478
Fragments arranged by size:
3478 1528 674
PvuI CG_AT'CG
Cuts at: 2001 2651 2001
Size: 650 5030
SacI G_AGCT'C
Cuts at: 2154 2154
Size: 5680
SacII CC_GC'GG
Cuts at: 2250 2250
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SapI GCTCTTCn'nnn_
Cuts at: 2505 2505
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 2354 2687 3028 4163 4844 5035 826
Size: 219 1309 333 341 1135 681 191 1471
Fragments arranged by size:
1471 1309 1135 681 341 333 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 2345 3007 1872
Size: 473 662 4545
Fragments arranged by size:
4545 662 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2556 3784 4685 4947 820
Size: 197 926 613 1228 901 262 1553
Fragments arranged by size:
1553 1228 926 901 613 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2840 3119 513
Size: 552 939 836 279 3074
Fragments arranged by size:
3074 939 836 552 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2901 3707 3724 3877 4062 4507 325
Size: 1255 1321 806 17 153 185 445 1498
Fragments arranged by size:
1498 1321 1255 806 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2017 2932 3926
Size: 274 80 33 51 1443 40 915 994
Cuts at: 3926 5638 96
Size: 1712 138
Fragments arranged by size:
1712 1443 994 915 274 138 80 51
40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 2030 3016 2030
Size: 986 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2898 2898
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 2556 1943
Size: 613 5067
XmaI C'CCGG_G
Cuts at: 1870 2343 3005 1870
Size: 473 662 4545
Fragments arranged by size:
4545 662 473
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI
SexAI SfiI SfoI SrfI SwaI