Restriction map for
pFN21AE5350
(Circular) (Six-base) MAPSORT of: pFN21AE5350.seq Check: 9352 from: 1 to: 5623
pFN21AE5350 5623 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2718 3433 278
Size: 53 83 186 936 1182 715 2468
Fragments arranged by size:
2468 1182 936 715 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2966 1937
Size: 1029 4594
Acc65I G'GTAC_C
Cuts at: 2944 2944
Size: 5623
AclI AA'CG_TT
Cuts at: 1563 3750 4123 1563
Size: 2187 373 3063
Fragments arranged by size:
3063 2187 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3686 5070 5338 1632
Size: 2054 1384 268 1917
Fragments arranged by size:
2054 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5426
AflIII A'CryG_T
Cuts at: 1203 2549 4522 5499 1203
Size: 1346 1973 977 1327
Fragments arranged by size:
1973 1346 1327 977
AgeI A'CCGG_T
Cuts at: 4454 4454
Size: 5623
AhdI GACnn_n'nnGTC
Cuts at: 2726 4352 2726
Size: 1626 3997
AleI CACnn'nnGTG
Cuts at: 1166 2243 1166
Size: 1077 4546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5623
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2403 2435 2403
Size: 32 5591
AlwNI CAG_nnn'CTG
Cuts at: 2704 2800 3366 4938 2704
Size: 96 566 1572 3389
Fragments arranged by size:
3389 1572 566 96
ApaLI G'TGCA_C
Cuts at: 3680 4836 3680
Size: 1156 4467
ApoI r'AATT_y
Cuts at: 1493 2932 3158 3310 5419 1493
Size: 1439 226 152 2109 1697
Fragments arranged by size:
2109 1697 1439 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4177 160
Size: 872 3145 1606
Fragments arranged by size:
3145 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 2624 2948 1870
Size: 73 235 446 324 4545
Fragments arranged by size:
4545 446 324 235 73
BamHI G'GATC_C
Cuts at: 2953 3320 2953
Size: 367 5256
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2272 2303 2888 2944 4399
Size: 325 217 627 485 31 585 56 1455
Cuts at: 4399 618
Size: 1842
Fragments arranged by size:
1842 1455 627 585 485 325 217 56
31
BanII G_rGCy'C
Cuts at: 1391 2076 2693 2942 1391
Size: 685 617 249 4072
Fragments arranged by size:
4072 685 617 249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4652
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3814 3848 1903
Size: 34 1877 34 3678
Fragments arranged by size:
3678 1877 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3515 4725 3515
Size: 1210 4413
BclI T'GATC_A
Cuts at: 1548 2247 1548
Size: 699 4924
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4234 136
Size: 107 122 71 631 605 2562 1525
Fragments arranged by size:
2562 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5618 5618
Size: 5623
BlpI GC'TnA_GC
Cuts at: 2070 3040 2070
Size: 970 4653
Bme1580I G_kGCm'C
Cuts at: 1790 2306 2893 3684 4840 1790
Size: 516 587 791 1156 2573
Fragments arranged by size:
2573 1156 791 587 516
BmgBI CAC'GTC
Cuts at: 1318 2782 1318
Size: 1464 4159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3354 4312 442
Size: 416 53 83 399 24 1937 958 1753
Fragments arranged by size:
1937 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2004 2114 2381 2621 4283 1239
Size: 765 110 267 240 1662 2579
Fragments arranged by size:
2579 1662 765 267 240 110
Bpu10I CC'TnA_GC
Cuts at: 2529 2692 2529
Size: 163 5460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3748 4613 4911 3748
Size: 865 298 4460
Fragments arranged by size:
4460 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4286 882
Size: 617 2787 2219
Fragments arranged by size:
2787 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3414 493
Size: 711 2210 2702
Fragments arranged by size:
2702 2210 711
BsaBI GATnn'nnATC
Cuts at: 5617 5617
Size: 5623
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2715 3430 3812 4447
Size: 53 83 186 936 1182 715 382 635
Cuts at: 4447 275
Size: 1451
Fragments arranged by size:
1451 1182 936 715 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4056 4454 4728 4875 5286 4056
Size: 398 274 147 411 4393
Fragments arranged by size:
4393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2245 2275 2403 2433 1178
Size: 30 165 30 842 30 128 30 4368
Fragments arranged by size:
4368 842 165 128 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2312 2462 2624 1186
Size: 931 195 150 162 4185
Fragments arranged by size:
4185 931 195 162 150
BseYI C'CCAG_C
Cuts at: 2743 4826 5322 2743
Size: 2083 496 3044
Fragments arranged by size:
3044 2083 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2266 2517 2804 2813 1163
Size: 573 530 251 287 9 3973
Fragments arranged by size:
3973 573 530 287 251 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3073 3834 4862 1540
Size: 275 186 1072 761 1028 2301
Fragments arranged by size:
2301 1072 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3684 3769 4840 3684
Size: 85 1071 4467
Fragments arranged by size:
4467 1071 85
BsmI GAATG_Cn'
Cuts at: 2567 3134 3227 2567
Size: 567 93 4963
Fragments arranged by size:
4963 567 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2306 2693 2893 2942 3684 3769
Size: 399 286 230 387 200 49 742 85
Cuts at: 3769 4840 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 286 230 200
85 49
BspEI T'CCGG_A
Cuts at: 5286 5286
Size: 5623
BspHI T'CATG_A
Cuts at: 3512 5242 3512
Size: 1730 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2422 2834 2978 844
Size: 1578 412 144 3489
Fragments arranged by size:
3489 1578 412 144
BsrBI CCG'CTC
Cuts at: 1394 1823 3510 1394
Size: 429 1687 3507
Fragments arranged by size:
3507 1687 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4118 4292 65
Size: 1183 2870 174 1396
Fragments arranged by size:
2870 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2768 4267 4454 1700
Size: 110 958 1499 187 2869
Fragments arranged by size:
2869 1499 958 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5623
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5623
BssSI C'ACGA_G
Cuts at: 3683 4695 3683
Size: 1012 4611
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2791 794
Size: 1997 3626
BstBI TT'CG_AA
Cuts at: 4427 4427
Size: 5623
BstEII G'GTnAC_C
Cuts at: 1184 2821 1184
Size: 1637 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 2350 2773 2350
Size: 423 5200
BstYI r'GATC_y
Cuts at: 1972 2953 3320 3705 3722 5174 5260 5272 5618
Size: 981 367 385 17 1452 86 12 346
Cuts at: 5618 1972
Size: 1977
Fragments arranged by size:
1977 1452 981 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2523 513
Size: 552 939 519 3613
Fragments arranged by size:
3613 939 552 519
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4884
Fragments arranged by size:
4884 683 56
BtsI GCAGTG_nn'
Cuts at: 3139 3933 3953 3139
Size: 794 20 4809
Fragments arranged by size:
4809 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5588
DraI TTT'AAA
Cuts at: 2927 2927
Size: 5623
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4630 809
Size: 507 3314 1802
Fragments arranged by size:
3314 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2108 2643 2802
Size: 54 1425 50 275 18 278 535 159
Cuts at: 2802 3070 3959 5588 8
Size: 268 889 1629 43
Fragments arranged by size:
1629 1425 889 535 278 275 268 159
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3070 1537
Size: 275 1258 4090
Fragments arranged by size:
4090 1258 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3553 1353
Size: 2200 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4210 4584 4730 387
Size: 1064 138 2621 374 146 1280
Fragments arranged by size:
2621 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2004 2114 2381 2621 3686 4283 5070
Size: 393 372 110 267 240 1065 597 787
Cuts at: 5070 5338 1239
Size: 268 1524
Fragments arranged by size:
1524 1065 787 597 393 372 268 267
240 110
EcoO109I rG'GnC_Cy
Cuts at: 2115 2354 2862 2115
Size: 239 508 4876
Fragments arranged by size:
4876 508 239
EcoRI G'AATT_C
Cuts at: 2932 3310 2932
Size: 378 5245
FspI TGC'GCA
Cuts at: 4129 4129
Size: 5623
HaeII r_GCGC'y
Cuts at: 1678 4770 1678
Size: 3092 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2175 2207 2712 2744 4264
Size: 32 135 32 603 32 505 32 1520
Cuts at: 4264 4296 4338 4370 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1520 603 505 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2967 3213 669
Size: 988 281 1029 246 3079
Fragments arranged by size:
3079 1029 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5623
HpaI GTT'AAC
Cuts at: 3213 3213
Size: 5623
KpnI G_GTAC'C
Cuts at: 2948 2948
Size: 5623
MfeI C'AATT_G
Cuts at: 3222 3222
Size: 5623
MluI A'CGCG_T
Cuts at: 5499 5499
Size: 5623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4737 4921 1305
Size: 3432 184 2007
Fragments arranged by size:
3432 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2110 2645 10
Size: 54 1425 343 278 535 2988
Fragments arranged by size:
2988 1425 535 343 278 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2226 2243 3581 3940 4099 518
Size: 648 1060 17 1338 359 159 2042
Fragments arranged by size:
2042 1338 1060 648 359 159 17
MspA1I CmG'CkG
Cuts at: 1606 2037 2641 3039 3716 4864 5109 1606
Size: 431 604 398 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 604 431 398 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2523 513
Size: 552 939 519 3613
Fragments arranged by size:
3613 939 552 519
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5623
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5623
NotI GC'GGCC_GC
Cuts at: 3070 3070
Size: 5623
NspI r_CATG'y
Cuts at: 1132 1229 2225 2553 2741 2981 4526 1132
Size: 97 996 328 188 240 1545 2229
Fragments arranged by size:
2229 1545 996 328 240 188 97
PasI CC'CwG_GG
Cuts at: 1348 2350 2857 1348
Size: 1002 507 4114
Fragments arranged by size:
4114 1002 507
PciI A'CATG_T
Cuts at: 2549 4522 2549
Size: 1973 3650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2524 1108
Size: 1416 4207
PfoI T'CCnGG_A
Cuts at: 2079 2079
Size: 5623
PmeI GTTT'AAAC
Cuts at: 2927 2927
Size: 5623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2403 2435 3692 3724 5205 5237 2403
Size: 32 1257 32 1481 32 2789
Fragments arranged by size:
2789 1481 1257 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2115 2354 2862 2115
Size: 239 508 4876
Fragments arranged by size:
4876 508 239
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5623
PsiI TTA'TAA
Cuts at: 3193 3193
Size: 5623
PstI C_TGCA'G
Cuts at: 830 2093 2175 2761 2975 830
Size: 1263 82 586 214 3478
Fragments arranged by size:
3478 1263 586 214 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5623
PvuII CAG'CTG
Cuts at: 2037 2641 2037
Size: 604 5019
SalI G'TCGA_C
Cuts at: 1936 2965 1936
Size: 1029 4594
SanDI GG'GwC_CC
Cuts at: 2354 2354
Size: 5623
SbfI CC_TGCA'GG
Cuts at: 2975 2975
Size: 5623
ScaI AGT'ACT
Cuts at: 3871 5583 3871
Size: 1712 3911
SfcI C'TryA_G
Cuts at: 826 1045 2089 2171 2757 2971 4106 4787 4978
Size: 219 1044 82 586 214 1135 681 191
Cuts at: 4978 826
Size: 1471
Fragments arranged by size:
1471 1135 1044 681 586 219 214 191
82
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5623
SmaI CCC'GGG
Cuts at: 1872 2180 2950 1872
Size: 308 770 4545
Fragments arranged by size:
4545 770 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3727 4628 4890 820
Size: 197 926 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3414 493
Size: 2921 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5623
SphI G_CATG'C
Cuts at: 1132 2225 2981 1132
Size: 1093 756 3774
Fragments arranged by size:
3774 1093 756
SspI AAT'ATT
Cuts at: 5 52 3547 5
Size: 47 3495 2081
Fragments arranged by size:
3495 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5623
StyI C'CwwG_G
Cuts at: 513 1065 2004 2111 2523 2646 3062 513
Size: 552 939 107 412 123 416 3074
Fragments arranged by size:
3074 939 552 416 412 123 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3650 3667 3820 4005 4450 325
Size: 1255 2070 17 153 185 445 1498
Fragments arranged by size:
2070 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3869 5581 96
Size: 274 80 33 51 1443 1892 1712 138
Fragments arranged by size:
1892 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2245 2277 5583 5615 2245
Size: 32 3306 32 2253
Fragments arranged by size:
3306 2253 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5623
XbaI T'CTAG_A
Cuts at: 2959 2959
Size: 5623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2530 2530
Size: 5623
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5623
XmaI C'CCGG_G
Cuts at: 1870 2178 2948 1870
Size: 308 770 4545
Fragments arranged by size:
4545 770 308
XmnI GAAnn'nnTTC
Cuts at: 3752 3752
Size: 5623
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2716 3431 276
Size: 53 83 186 936 1182 715 2468
Fragments arranged by size:
2468 1182 936 715 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI