Restriction map for
pFN21AE5347
(Circular) (Six-base) MAPSORT of: pFN21AE5347.seq Check: 3663 from: 1 to: 5569
pFN21AE5347 5569 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2289 2912 1937
Size: 352 623 4594
Fragments arranged by size:
4594 623 352
Acc65I G'GTAC_C
Cuts at: 2136 2890 2136
Size: 754 4815
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2792 3632 5016 5284 1632
Size: 1160 840 1384 268 1917
Fragments arranged by size:
1917 1384 1160 840 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 2597 4298 2597
Size: 1701 3868
AleI CACnn'nnGTG
Cuts at: 1166 2645 1166
Size: 1479 4090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 2338 3312 4884 2338
Size: 974 1572 3023
Fragments arranged by size:
3023 1572 974
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2878 3104 3256 5365 1493
Size: 1385 226 152 2109 1697
Fragments arranged by size:
2109 1697 1385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2500 2894 1870
Size: 73 557 394 4545
Fragments arranged by size:
4545 557 394 73
BamHI G'GATC_C
Cuts at: 2899 3266 2899
Size: 367 5202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2136 2890 4345 618
Size: 325 217 627 349 754 1455 1842
Fragments arranged by size:
1842 1455 754 627 349 325 217
BanII G_rGCy'C
Cuts at: 1391 2172 2888 1391
Size: 781 716 4072
Fragments arranged by size:
4072 781 716
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2014 928
Size: 971 115 4483
Fragments arranged by size:
4483 971 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3760 3794 1903
Size: 34 50 34 1739 34 3678
Fragments arranged by size:
3678 1739 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2843 3461 4671 2843
Size: 618 1210 3741
Fragments arranged by size:
3741 1210 618
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5569
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4180 136
Size: 107 122 71 631 605 2508 1525
Fragments arranged by size:
2508 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2986 2986
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1790 2581 2704 3630 4786 1790
Size: 791 123 926 1156 2573
Fragments arranged by size:
2573 1156 926 791 123
BmgBI CAC'GTC
Cuts at: 1318 2038 1318
Size: 720 4849
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3300 4258 442
Size: 416 53 83 399 24 1883 958 1753
Fragments arranged by size:
1883 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2352 2352
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2428 2462 2689 4229 1239
Size: 1189 34 227 1540 2579
Fragments arranged by size:
2579 1540 1189 227 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3694 4559 4857 3694
Size: 865 298 4406
Fragments arranged by size:
4406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4232 882
Size: 617 2733 2219
Fragments arranged by size:
2733 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2278 2632 3360 493
Size: 711 1074 354 728 2702
Fragments arranged by size:
2702 1074 728 711 354
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2368 3376 3758 4393
Size: 53 83 186 936 835 1008 382 635
Cuts at: 4393 275
Size: 1451
Fragments arranged by size:
1451 1008 936 835 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2133 4002 4400 4674 4821 5232 2133
Size: 1869 398 274 147 411 2470
Fragments arranged by size:
2470 1869 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 2843 2849 2873
Size: 30 165 30 1032 30 378 6 24
Cuts at: 2873 2879 1178
Size: 6 3868
Fragments arranged by size:
3868 1032 378 165 30 30 30 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2792 2795 2851 2854 1186
Size: 1606 3 56 3 3901
Fragments arranged by size:
3901 1606 56 3 3
BseYI C'CCAG_C
Cuts at: 2177 2299 2798 4772 5268 2177
Size: 122 499 1974 496 2478
Fragments arranged by size:
2478 1974 499 496 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 2473 2573 1163
Size: 573 720 17 100 4159
Fragments arranged by size:
4159 720 573 100 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2217 3019 3780 4808 1540
Size: 275 186 216 802 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 275 216 186
BsiHKAI G_wGCw'C
Cuts at: 3630 3715 4786 3630
Size: 85 1071 4413
Fragments arranged by size:
4413 1071 85
BsmI GAATG_Cn'
Cuts at: 3080 3173 3080
Size: 93 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2581 2704 2888 3630 3715 4786
Size: 399 382 409 123 184 742 85 1071
Cuts at: 4786 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 409 399 382 184 123
85
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 3458 5188 3458
Size: 1730 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2924 844
Size: 2080 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3456 1394
Size: 429 1633 3507
Fragments arranged by size:
3507 1633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4064 4238 65
Size: 1183 2816 174 1396
Fragments arranged by size:
2816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2244 4213 4400 1700
Size: 110 434 1969 187 2869
Fragments arranged by size:
2869 1969 434 187 110
BsrGI T'GTAC_A
Cuts at: 96 2235 96
Size: 2139 3430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 3629 4641 3629
Size: 1012 4557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 2182 1184
Size: 998 4571
BstYI r'GATC_y
Cuts at: 1972 2899 3266 3651 3668 5120 5206 5218 5564
Size: 927 367 385 17 1452 86 12 346
Cuts at: 5564 1972
Size: 1977
Fragments arranged by size:
1977 1452 927 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2290 2290
Size: 5569
BtgI C'CryG_G
Cuts at: 513 1065 2164 513
Size: 552 1099 3918
Fragments arranged by size:
3918 1099 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2688 487
Size: 683 56 1462 3368
Fragments arranged by size:
3368 1462 683 56
BtsI GCAGTG_nn'
Cuts at: 3085 3879 3899 3085
Size: 794 20 4755
Fragments arranged by size:
4755 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2214 2349
Size: 54 1425 50 275 18 363 21 135
Cuts at: 2349 3016 3905 5534 8
Size: 667 889 1629 43
Fragments arranged by size:
1629 1425 889 667 363 275 135 54
50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2214 3016 1537
Size: 275 402 802 4090
Fragments arranged by size:
4090 802 402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2515 3499 1353
Size: 1162 984 3423
Fragments arranged by size:
3423 1162 984
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2428 2462 2689 2792 3632 4229 5016
Size: 393 796 34 227 103 840 597 787
Cuts at: 5016 5284 1239
Size: 268 1524
Fragments arranged by size:
1524 840 796 787 597 393 268 227
103 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2693 2825 2693
Size: 132 5437
EcoO109I rG'GnC_Cy
Cuts at: 2304 2304
Size: 5569
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 2546 4716 1678
Size: 868 2170 2531
Fragments arranged by size:
2531 2170 868
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2353 2385 2583 2615 4210
Size: 32 135 32 781 32 198 32 1595
Cuts at: 4210 4242 4284 4316 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1595 781 198 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2419 2817 2913 3159 669
Size: 988 281 481 398 96 246 3079
Fragments arranged by size:
3079 988 481 398 281 246 96
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KpnI G_GTAC'C
Cuts at: 2140 2894 2140
Size: 754 4815
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2549 4683 4867 1305
Size: 1244 2134 184 2007
Fragments arranged by size:
2134 2007 1244 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 10
Size: 54 1425 343 519 3228
Fragments arranged by size:
3228 1425 519 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 3527 3886 4045 518
Size: 648 1479 882 359 159 2042
Fragments arranged by size:
2042 1479 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2440 2985 3662 4810 5055 1606
Size: 405 429 545 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 545 429 405 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5017
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2927 4472 1132
Size: 97 1698 1545 2229
Fragments arranged by size:
2229 1698 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2821 1348
Size: 1473 4096
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5569
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2304 2304
Size: 5569
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2081 2113 2081
Size: 32 5537
PstI C_TGCA'G
Cuts at: 830 2697 2921 830
Size: 1867 224 3478
Fragments arranged by size:
3478 1867 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2440 2440
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SbfI CC_TGCA'GG
Cuts at: 2697 2921 2697
Size: 224 5345
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SexAI A'CCwGG_T
Cuts at: 2229 2229
Size: 5569
SfcI C'TryA_G
Cuts at: 826 1045 2693 2917 4052 4733 4924 826
Size: 219 1648 224 1135 681 191 1471
Fragments arranged by size:
1648 1471 1135 681 224 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3673 4574 4836 820
Size: 197 926 1730 901 262 1553
Fragments arranged by size:
1730 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 3008 513
Size: 552 1943 3074
Fragments arranged by size:
3074 1943 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 2416 3596 3613 3766 3951 4396
Size: 1255 597 239 1180 17 153 185 445
Cuts at: 4396 325
Size: 1498
Fragments arranged by size:
1498 1255 1180 597 445 239 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 2355 2523
Size: 274 80 33 51 1443 258 120 168
Cuts at: 2523 3815 5527 96
Size: 1292 1712 138
Fragments arranged by size:
1712 1443 1292 274 258 168 138 120
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 2849 2881 5529 5561 2435
Size: 32 382 32 2648 32 2443
Fragments arranged by size:
2648 2443 382 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2843 2843
Size: 5569
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI