Restriction map for
pFN21AE5342
(Circular) (Six-base) MAPSORT of: pFN21AE5342.seq Check: 8536 from: 1 to: 5524
pFN21AE5342 5524 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2684 2867 1937
Size: 747 183 4594
Fragments arranged by size:
4594 747 183
Acc65I G'GTAC_C
Cuts at: 2845 2845
Size: 5524
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 3587 4971 5239 1632
Size: 753 1202 1384 268 1917
Fragments arranged by size:
1917 1384 1202 753 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 2506 4423 5400 1203
Size: 1303 1917 977 1327
Fragments arranged by size:
1917 1327 1303 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 3267 4839 3267
Size: 1572 3952
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2663 2713 2833 3059 3211 5320 1493
Size: 1170 50 120 226 152 2109 1697
Fragments arranged by size:
2109 1697 1170 226 152 120 50
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2695 2849 1870
Size: 73 752 154 4545
Fragments arranged by size:
4545 752 154 73
BamHI G'GATC_C
Cuts at: 2637 2854 3221 2637
Size: 217 367 4940
Fragments arranged by size:
4940 367 217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2845 4300 618
Size: 325 217 627 1058 1455 1842
Fragments arranged by size:
1842 1455 1058 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2843 1391
Size: 1452 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 1903
Size: 34 1778 34 3678
Fragments arranged by size:
3678 1778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2040 3416 4626 2040
Size: 1376 1210 2938
Fragments arranged by size:
2938 1376 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4135 136
Size: 107 122 71 631 605 2463 1525
Fragments arranged by size:
2463 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 3585 4741 1790
Size: 1795 1156 2573
Fragments arranged by size:
2573 1795 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2228 3255 4213
Size: 416 53 83 399 24 811 1027 958
Cuts at: 4213 442
Size: 1753
Fragments arranged by size:
1753 1027 958 811 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 4184 1239
Size: 1259 1686 2579
Fragments arranged by size:
2579 1686 1259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 3649 4514 4812 2663
Size: 986 865 298 3375
Fragments arranged by size:
3375 986 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2474 4187 882
Size: 617 975 1713 2219
Fragments arranged by size:
2219 1713 975 617
BsaAI yAC'GTr
Cuts at: 493 1204 3315 493
Size: 711 2111 2702
Fragments arranged by size:
2702 2111 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3331 3713 4348 275
Size: 53 83 186 936 1798 382 635 1451
Fragments arranged by size:
1798 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3957 4355 4629 4776 5187 3957
Size: 398 274 147 411 4294
Fragments arranged by size:
4294 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5299
Fragments arranged by size:
5299 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2014 2148 1186
Size: 828 134 4562
Fragments arranged by size:
4562 828 134
BseYI C'CCAG_C
Cuts at: 2189 4727 5223 2189
Size: 2538 496 2490
Fragments arranged by size:
2538 2490 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4951
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2974 3735 4763 1540
Size: 275 186 973 761 1028 2301
Fragments arranged by size:
2301 1028 973 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3585 3670 4741 3585
Size: 85 1071 4368
Fragments arranged by size:
4368 1071 85
BsmI GAATG_Cn'
Cuts at: 2128 2803 3035 3128 2128
Size: 675 232 93 4524
Fragments arranged by size:
4524 675 232 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2843 3585 3670 4741 1391
Size: 399 1053 742 85 1071 2174
Fragments arranged by size:
2174 1071 1053 742 399 85
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 2458 3413 5143 2458
Size: 955 1730 2839
Fragments arranged by size:
2839 1730 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2879 844
Size: 2035 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3411 1394
Size: 429 1588 3507
Fragments arranged by size:
3507 1588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4168 4355 1700
Size: 110 2358 187 2869
Fragments arranged by size:
2869 2358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 3584 4596 3584
Size: 1012 4512
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5524
BstBI TT'CG_AA
Cuts at: 2710 4328 2710
Size: 1618 3906
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2229 2637 2854 3221 3606 3623 5075 5161
Size: 257 408 217 367 385 17 1452 86
Cuts at: 5161 5173 5519 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 408 385 367 346 257 217
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2255 2727 513
Size: 552 1190 472 3310
Fragments arranged by size:
3310 1190 552 472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4785
Fragments arranged by size:
4785 683 56
BtsI GCAGTG_nn'
Cuts at: 3040 3834 3854 3040
Size: 794 20 4710
Fragments arranged by size:
4710 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5489
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2252 2971 3860
Size: 54 1425 50 275 18 422 719 889
Cuts at: 3860 5489 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 719 422 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2971 1537
Size: 275 1159 4090
Fragments arranged by size:
4090 1159 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3454 1353
Size: 2101 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2385 2498 3587 4184 4971 5239 1239
Size: 393 753 113 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 787 753 597 393 268 113
EcoO109I rG'GnC_Cy
Cuts at: 2019 2019
Size: 5524
EcoRI G'AATT_C
Cuts at: 2663 2833 3211 2663
Size: 170 378 4976
Fragments arranged by size:
4976 378 170
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 4671 1678
Size: 2993 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373
Size: 32 135 32 2593 32 42 32 2626
Fragments arranged by size:
2626 2593 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2514 2868 3114 669
Size: 988 281 576 354 246 3079
Fragments arranged by size:
3079 988 576 354 281 246
HindIII A'AGCT_T
Cuts at: 748 2324 748
Size: 1576 3948
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KpnI G_GTAC'C
Cuts at: 2849 2849
Size: 5524
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 2506 5400 2506
Size: 2894 2630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 4822 1305
Size: 3333 184 2007
Fragments arranged by size:
3333 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3702
Fragments arranged by size:
3702 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2819 3482 3841 4000 518
Size: 648 1653 663 359 159 2042
Fragments arranged by size:
2042 1653 663 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2940 3617 4765 5010 1606
Size: 511 823 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 677 511 245
NcoI C'CATG_G
Cuts at: 513 1065 2727 513
Size: 552 1662 3310
Fragments arranged by size:
3310 1662 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2882 4427 1132
Size: 97 1653 1545 2229
Fragments arranged by size:
2229 1653 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2609 1108
Size: 1501 4023
PfoI T'CCnGG_A
Cuts at: 2054 2054
Size: 5524
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2313 1613
Size: 700 4824
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2876 830
Size: 2046 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
PvuII CAG'CTG
Cuts at: 2117 2117
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SfcI C'TryA_G
Cuts at: 826 1045 2658 2872 4007 4688 4879 826
Size: 219 1613 214 1135 681 191 1471
Fragments arranged by size:
1613 1471 1135 681 219 214 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2851 1872
Size: 979 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2642 3628 4529 4791 820
Size: 197 926 699 986 901 262 1553
Fragments arranged by size:
1553 986 926 901 699 262 197
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SspI AAT'ATT
Cuts at: 5 52 2756 3448 5
Size: 47 2704 692 2081
Fragments arranged by size:
2704 2081 692 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5524
StyI C'CwwG_G
Cuts at: 513 1065 2493 2727 2963 513
Size: 552 1428 234 236 3074
Fragments arranged by size:
3074 1428 552 236 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2186 2203 2670 3551 3568 3721 3906
Size: 1255 606 17 467 881 17 153 185
Cuts at: 3906 4351 325
Size: 445 1498
Fragments arranged by size:
1498 1255 881 606 467 445 185 153
17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3770 5482 96
Size: 274 80 33 51 1443 1793 1712 138
Fragments arranged by size:
1793 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2849 1870
Size: 979 4545
XmnI GAAnn'nnTTC
Cuts at: 3653 3653
Size: 5524
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI