Restriction map for
pFN21AE5340
(Circular) (Six-base) MAPSORT of: pFN21AE5340.seq Check: 4287 from: 1 to: 5494
pFN21AE5340 5494 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 2772 3557 4941 5209 1632
Size: 419 721 785 1384 268 1917
Fragments arranged by size:
1917 1384 785 721 419 268
AflII C'TTAA_G
Cuts at: 820 1017 2159 820
Size: 197 1142 4155
Fragments arranged by size:
4155 1142 197
AflIII A'CryG_T
Cuts at: 1203 4393 5370 1203
Size: 3190 977 1327
Fragments arranged by size:
3190 1327 977
AgeI A'CCGG_T
Cuts at: 2075 2102 4325 2075
Size: 27 2223 3244
Fragments arranged by size:
3244 2223 27
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5494
AlwNI CAG_nnn'CTG
Cuts at: 3237 4809 3237
Size: 1572 3922
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2296 2803 3029 3181 5290 1493
Size: 803 507 226 152 2109 1697
Fragments arranged by size:
2109 1697 803 507 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2721 2819 1870
Size: 73 778 98 4545
Fragments arranged by size:
4545 778 98 73
AvrII C'CTAG_G
Cuts at: 2569 2569
Size: 5494
BamHI G'GATC_C
Cuts at: 2824 3191 2824
Size: 367 5127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2604 2815 4270 618
Size: 325 217 627 817 211 1455 1842
Fragments arranged by size:
1842 1455 817 627 325 217 211
BanII G_rGCy'C
Cuts at: 1391 2813 1391
Size: 1422 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3685 3719 1903
Size: 34 1748 34 3678
Fragments arranged by size:
3678 1748 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2744 3386 4596 2744
Size: 642 1210 3642
Fragments arranged by size:
3642 1210 642
BclI T'GATC_A
Cuts at: 1548 2586 1548
Size: 1038 4456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4105 136
Size: 107 122 71 631 605 2433 1525
Fragments arranged by size:
2433 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2388 5489 2388
Size: 3101 2393
BlpI GC'TnA_GC
Cuts at: 2911 2911
Size: 5494
Bme1580I G_kGCm'C
Cuts at: 1790 2402 3555 4711 1790
Size: 612 1153 1156 2573
Fragments arranged by size:
2573 1156 1153 612
BmgBI CAC'GTC
Cuts at: 1318 2623 1318
Size: 1305 4189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3225 4183 442
Size: 416 53 83 399 24 1808 958 1753
Fragments arranged by size:
1808 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 2144
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2577 4154 1239
Size: 1338 1577 2579
Fragments arranged by size:
2579 1577 1338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3619 4484 4782 3619
Size: 865 298 4331
Fragments arranged by size:
4331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4157 882
Size: 617 2658 2219
Fragments arranged by size:
2658 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2632 3285 493
Size: 711 1428 653 2702
Fragments arranged by size:
2702 1428 711 653
BsaBI GATnn'nnATC
Cuts at: 5488 5488
Size: 5494
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3301 3683 4318 275
Size: 53 83 186 936 1768 382 635 1451
Fragments arranged by size:
1768 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2075 2102 3927 4325 4599 4746 5157 2075
Size: 27 1825 398 274 147 411 2412
Fragments arranged by size:
2412 1825 411 398 274 147 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2169 2199 2348 2378 1178
Size: 30 165 30 766 30 149 30 4294
Fragments arranged by size:
4294 766 165 149 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2079 2369 1186
Size: 893 290 4311
Fragments arranged by size:
4311 893 290
BseYI C'CCAG_C
Cuts at: 4697 5193 4697
Size: 496 4998
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4921
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3555 3640 4711 3555
Size: 85 1071 4338
Fragments arranged by size:
4338 1071 85
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2402 2813 3555 3640 4711 1391
Size: 399 612 411 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 612 411 399 85
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 2428 3383 5113 2428
Size: 955 1730 2809
Fragments arranged by size:
2809 1730 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2849 844
Size: 2005 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3381 1394
Size: 429 1558 3507
Fragments arranged by size:
3507 1558 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2745 3989 4163 65
Size: 1183 1497 1244 174 1396
Fragments arranged by size:
1497 1396 1244 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 2102 4138 4325 1700
Size: 110 265 27 2036 187 2869
Fragments arranged by size:
2869 2036 265 187 110 27
BsrGI T'GTAC_A
Cuts at: 96 2335 96
Size: 2239 3255
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 2010 3554 4566 2010
Size: 1544 1012 2938
Fragments arranged by size:
2938 1544 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 794
Size: 1265 4229
BstBI TT'CG_AA
Cuts at: 4298 4298
Size: 5494
BstEII G'GTnAC_C
Cuts at: 1184 2537 1184
Size: 1353 4141
BstXI CCAn_nnnn'nTGG
Cuts at: 2055 2055
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2388 2612 2824 3191 3576 3593 5045 5131
Size: 416 224 212 367 385 17 1452 86
Cuts at: 5131 5143 5489 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 416 385 367 346 224 212
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2147 2352 2147
Size: 205 5289
BtgI C'CryG_G
Cuts at: 513 1065 2004 2705 513
Size: 552 939 701 3302
Fragments arranged by size:
3302 939 701 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4755
Fragments arranged by size:
4755 683 56
BtsI GCAGTG_nn'
Cuts at: 2794 3010 3804 3824 2794
Size: 216 794 20 4464
Fragments arranged by size:
4464 794 216 20
ClaI AT'CG_AT
Cuts at: 1554 2220 1554
Size: 666 4828
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4501 809
Size: 507 3185 1802
Fragments arranged by size:
3185 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2941 3830 5459
Size: 54 1425 50 275 18 1111 889 1629
Cuts at: 5459 8
Size: 43
Fragments arranged by size:
1629 1425 1111 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2721 2748 3424 1353
Size: 1368 27 676 3423
Fragments arranged by size:
3423 1368 676 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2726 4081 4455 4601 387
Size: 1064 138 1137 1355 374 146 1280
Fragments arranged by size:
1355 1280 1137 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2577 2772 3557 4154 4941 5209
Size: 393 419 526 195 785 597 787 268
Cuts at: 5209 1239
Size: 1524
Fragments arranged by size:
1524 787 785 597 526 419 393 268
195
EcoNI CCTnn'n_nnAGG
Cuts at: 2465 2465
Size: 5494
EcoRI G'AATT_C
Cuts at: 2803 3181 2803
Size: 378 5116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 5494
FspI TGC'GCA
Cuts at: 4000 4000
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 4641 1678
Size: 2963 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 4135 4167 4209
Size: 32 135 32 538 32 1993 32 42
Cuts at: 4209 4241 1373
Size: 32 2626
Fragments arranged by size:
2626 1993 538 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3084 669
Size: 988 281 900 246 3079
Fragments arranged by size:
3079 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2697 4608 4792 1305
Size: 1392 1911 184 2007
Fragments arranged by size:
2007 1911 1392 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3672
Fragments arranged by size:
3672 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 3452 3811 3970 518
Size: 648 1302 984 359 159 2042
Fragments arranged by size:
2042 1302 984 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 2910 3587 4735 4980 1606
Size: 600 704 677 1148 245 2120
Fragments arranged by size:
2120 1148 704 677 600 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2705 513
Size: 552 939 701 3302
Fragments arranged by size:
3302 939 701 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2203 2852 4397 1132
Size: 97 974 649 1545 2229
Fragments arranged by size:
2229 1545 974 649 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5494
PciI A'CATG_T
Cuts at: 4393 4393
Size: 5494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PmlI CAC'GTG
Cuts at: 2632 2632
Size: 5494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2846 830
Size: 2016 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SbfI CC_TGCA'GG
Cuts at: 2846 2846
Size: 5494
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2842 3977 4658 4849 826
Size: 219 1797 1135 681 191 1471
Fragments arranged by size:
1797 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 3598 4499 4761 820
Size: 197 926 216 1439 901 262 1553
Fragments arranged by size:
1553 1439 926 901 262 216 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2203 2852 1132
Size: 1071 649 3774
Fragments arranged by size:
3774 1071 649
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5494
StyI C'CwwG_G
Cuts at: 513 1065 2004 2319 2569 2705 2933 513
Size: 552 939 315 250 136 228 3074
Fragments arranged by size:
3074 939 552 315 250 228 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3521 3538 3691 3876 4321 325
Size: 1255 1941 17 153 185 445 1498
Fragments arranged by size:
1941 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2335 2364 3740
Size: 274 80 33 51 1443 358 29 1376
Cuts at: 3740 5452 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1376 358 274 138 80 51
33 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5454 5486 5454
Size: 32 5462
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 3623 3623
Size: 5494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI