Restriction map for
pFN21AE5333
(Circular) (Six-base) MAPSORT of: pFN21AE5333.seq Check: 3489 from: 1 to: 5530
pFN21AE5333 5530 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2814 2814
Size: 5530
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3340 278
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2873 1937
Size: 936 4594
Acc65I G'GTAC_C
Cuts at: 2851 2851
Size: 5530
AclI AA'CG_TT
Cuts at: 1563 3657 4030 1563
Size: 2094 373 3063
Fragments arranged by size:
3063 2094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 3593 4977 5245 1632
Size: 674 1287 1384 268 1917
Fragments arranged by size:
1917 1384 1287 674 268
AfeI AGC'GCT
Cuts at: 2612 2612
Size: 5530
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5333
AflIII A'CryG_T
Cuts at: 1203 2751 4429 5406 1203
Size: 1548 1678 977 1327
Fragments arranged by size:
1678 1548 1327 977
AgeI A'CCGG_T
Cuts at: 4361 4361
Size: 5530
AhdI GACnn_n'nnGTC
Cuts at: 4259 4259
Size: 5530
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5530
AlwNI CAG_nnn'CTG
Cuts at: 2673 2698 3273 4845 2673
Size: 25 575 1572 3358
Fragments arranged by size:
3358 1572 575 25
ApaLI G'TGCA_C
Cuts at: 3587 4743 3587
Size: 1156 4374
ApoI r'AATT_y
Cuts at: 1493 2839 3065 3217 5326 1493
Size: 1346 226 152 2109 1697
Fragments arranged by size:
2109 1697 1346 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4084 160
Size: 872 3052 1606
Fragments arranged by size:
3052 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
AvaI C'yCGr_G
Cuts at: 1870 1943 2686 2705 2855 1870
Size: 73 743 19 150 4545
Fragments arranged by size:
4545 743 150 73 19
BamHI G'GATC_C
Cuts at: 2860 3227 2860
Size: 367 5163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2715 2851 4306 618
Size: 325 217 627 928 136 1455 1842
Fragments arranged by size:
1842 1455 928 627 325 217 136
BanII G_rGCy'C
Cuts at: 1391 2732 2849 1391
Size: 1341 117 4072
Fragments arranged by size:
4072 1341 117
BbeI G_GCGC'C
Cuts at: 2719 2719
Size: 5530
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2294 928
Size: 971 395 4164
Fragments arranged by size:
4164 971 395
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3721 3755 1903
Size: 34 1784 34 3678
Fragments arranged by size:
3678 1784 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3422 4632 3422
Size: 1210 4320
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4141 136
Size: 107 122 71 631 605 2469 1525
Fragments arranged by size:
2469 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5525 5525
Size: 5530
BlpI GC'TnA_GC
Cuts at: 2039 2947 2039
Size: 908 4622
Bme1580I G_kGCm'C
Cuts at: 1790 3591 4747 1790
Size: 1801 1156 2573
Fragments arranged by size:
2573 1801 1156
BmgBI CAC'GTC
Cuts at: 1318 2752 1318
Size: 1434 4096
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3261 4219 442
Size: 416 53 83 399 24 1844 958 1753
Fragments arranged by size:
1844 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2449 2728 4190 1239
Size: 1210 279 1462 2579
Fragments arranged by size:
2579 1462 1210 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3655 4520 4818 3655
Size: 865 298 4367
Fragments arranged by size:
4367 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4193 882
Size: 617 2694 2219
Fragments arranged by size:
2694 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3321 493
Size: 711 2117 2702
Fragments arranged by size:
2702 2117 711
BsaBI GATnn'nnATC
Cuts at: 5524 5524
Size: 5530
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2716 3337 3719 4354
Size: 53 83 186 936 1183 621 382 635
Cuts at: 4354 275
Size: 1451
Fragments arranged by size:
1451 1183 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2245 3963 4361 4635 4782 5193 2245
Size: 1718 398 274 147 411 2582
Fragments arranged by size:
2582 1718 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2615 2645 1178
Size: 30 165 30 1212 30 4063
Fragments arranged by size:
4063 1212 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 2639 1186
Size: 1450 3 4077
Fragments arranged by size:
4077 1450 3
BseYI C'CCAG_C
Cuts at: 2495 4733 5229 2495
Size: 2238 496 2796
Fragments arranged by size:
2796 2238 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2792 1163
Size: 573 414 642 3901
Fragments arranged by size:
3901 642 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2725 2980 3741 4769 1540
Size: 275 186 724 255 761 1028 2301
Fragments arranged by size:
2301 1028 761 724 275 255 186
BsiHKAI G_wGCw'C
Cuts at: 3591 3676 4747 3591
Size: 85 1071 4374
Fragments arranged by size:
4374 1071 85
BsmI GAATG_Cn'
Cuts at: 3041 3134 3041
Size: 93 5437
BsmBI CGTCTCn'nnnn_
Cuts at: 2669 2669
Size: 5530
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2732 2849 3591 3676 4747 1391
Size: 399 942 117 742 85 1071 2174
Fragments arranged by size:
2174 1071 942 742 399 117 85
BspEI T'CCGG_A
Cuts at: 5193 5193
Size: 5530
BspHI T'CATG_A
Cuts at: 2484 3419 5149 2484
Size: 935 1730 2865
Fragments arranged by size:
2865 1730 935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2189 2814 2885 844
Size: 1345 625 71 3489
Fragments arranged by size:
3489 1345 625 71
BsrBI CCG'CTC
Cuts at: 1394 1823 3417 1394
Size: 429 1594 3507
Fragments arranged by size:
3507 1594 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4025 4199 65
Size: 1183 2777 174 1396
Fragments arranged by size:
2777 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2171 2579 4174 4361 1700
Size: 110 361 408 1595 187 2869
Fragments arranged by size:
2869 1595 408 361 187 110
BsrGI T'GTAC_A
Cuts at: 96 2106 96
Size: 2010 3520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5530
BssSI C'ACGA_G
Cuts at: 2333 3590 4602 2333
Size: 1257 1012 3261
Fragments arranged by size:
3261 1257 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5530
BstBI TT'CG_AA
Cuts at: 4334 4334
Size: 5530
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5530
BstYI r'GATC_y
Cuts at: 1972 2860 3227 3612 3629 5081 5167 5179 5525
Size: 888 367 385 17 1452 86 12 346
Cuts at: 5525 1972
Size: 1977
Fragments arranged by size:
1977 1452 888 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2316 2719 513
Size: 552 1251 403 3324
Fragments arranged by size:
3324 1251 552 403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2065 487
Size: 683 56 839 3952
Fragments arranged by size:
3952 839 683 56
BtsI GCAGTG_nn'
Cuts at: 2011 3046 3840 3860 2011
Size: 1035 794 20 3681
Fragments arranged by size:
3681 1035 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5530
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5495
DraI TTT'AAA
Cuts at: 2834 2834
Size: 5530
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4537 809
Size: 507 3221 1802
Fragments arranged by size:
3221 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2365 2722
Size: 54 1425 50 275 18 471 64 357
Cuts at: 2722 2977 3866 5495 8
Size: 255 889 1629 43
Fragments arranged by size:
1629 1425 889 471 357 275 255 64
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2722 2977 1537
Size: 275 910 255 4090
Fragments arranged by size:
4090 910 275 255
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 3460 1353
Size: 980 1127 3423
Fragments arranged by size:
3423 1127 980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2363 4117 4491 4637 387
Size: 1064 138 774 1754 374 146 1280
Fragments arranged by size:
1754 1280 1064 774 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2449 2728 3593 4190 4977 5245
Size: 393 674 143 279 865 597 787 268
Cuts at: 5245 1239
Size: 1524
Fragments arranged by size:
1524 865 787 674 597 393 279 268
143
EcoRI G'AATT_C
Cuts at: 2839 3217 2839
Size: 378 5152
FspI TGC'GCA
Cuts at: 2297 4036 2297
Size: 1739 3791
FspAI rTGC'GCAy
Cuts at: 2297 2297
Size: 5530
HaeII r_GCGC'y
Cuts at: 1678 2614 2719 4677 1678
Size: 936 105 1958 2531
Fragments arranged by size:
2531 1958 936 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2620 2652 4171 4203 4245
Size: 32 135 32 1048 32 1519 32 42
Cuts at: 4245 4277 1373
Size: 32 2626
Fragments arranged by size:
2626 1519 1048 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2874 3120 669
Size: 988 281 936 246 3079
Fragments arranged by size:
3079 988 936 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5530
HpaI GTT'AAC
Cuts at: 3120 3120
Size: 5530
KasI G'GCGC_C
Cuts at: 2715 2715
Size: 5530
KpnI G_GTAC'C
Cuts at: 2855 2855
Size: 5530
MfeI C'AATT_G
Cuts at: 3129 3129
Size: 5530
MluI A'CGCG_T
Cuts at: 5406 5406
Size: 5530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2703 2760 4644 4828 1305
Size: 1398 57 1884 184 2007
Fragments arranged by size:
2007 1884 1398 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3708
Fragments arranged by size:
3708 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2762 3488 3847 4006 518
Size: 648 1596 726 359 159 2042
Fragments arranged by size:
2042 1596 726 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2721 2946 3623 4771 5016 1606
Size: 1115 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 1115 677 245 225
NaeI GCC'GGC
Cuts at: 2173 2581 2173
Size: 408 5122
NarI GG'CG_CC
Cuts at: 2716 2716
Size: 5530
NcoI C'CATG_G
Cuts at: 513 1065 2316 513
Size: 552 1251 3727
Fragments arranged by size:
3727 1251 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5530
NgoMIV G'CCGG_C
Cuts at: 2171 2579 2171
Size: 408 5122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5530
NotI GC'GGCC_GC
Cuts at: 2977 2977
Size: 5530
NspI r_CATG'y
Cuts at: 1132 1229 2888 4433 1132
Size: 97 1659 1545 2229
Fragments arranged by size:
2229 1659 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5530
PciI A'CATG_T
Cuts at: 4429 4429
Size: 5530
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5530
PmeI GTTT'AAAC
Cuts at: 2834 2834
Size: 5530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 5112 5144 3599
Size: 32 1481 32 3985
Fragments arranged by size:
3985 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5530
PsiI TTA'TAA
Cuts at: 3100 3100
Size: 5530
PstI C_TGCA'G
Cuts at: 830 2018 2703 2882 830
Size: 1188 685 179 3478
Fragments arranged by size:
3478 1188 685 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5530
SacII CC_GC'GG
Cuts at: 2722 2722
Size: 5530
SalI G'TCGA_C
Cuts at: 1936 2872 1936
Size: 936 4594
SbfI CC_TGCA'GG
Cuts at: 2882 2882
Size: 5530
ScaI AGT'ACT
Cuts at: 3778 5490 3778
Size: 1712 3818
SfcI C'TryA_G
Cuts at: 826 1045 2014 2699 2878 4013 4694 4885 826
Size: 219 969 685 179 1135 681 191 1471
Fragments arranged by size:
1471 1135 969 685 681 219 191 179
SfoI GGC'GCC
Cuts at: 2717 2717
Size: 5530
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5530
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5530
SmaI CCC'GGG
Cuts at: 1872 2857 1872
Size: 985 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3634 4535 4797 820
Size: 197 926 1691 901 262 1553
Fragments arranged by size:
1691 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3321 493
Size: 2828 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5530
SphI G_CATG'C
Cuts at: 1132 2888 1132
Size: 1756 3774
SspI AAT'ATT
Cuts at: 5 52 2234 2281 3454 5
Size: 47 2182 47 1173 2081
Fragments arranged by size:
2182 2081 1173 47 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5530
StyI C'CwwG_G
Cuts at: 513 1065 2316 2436 2969 513
Size: 552 1251 120 533 3074
Fragments arranged by size:
3074 1251 552 533 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3557 3574 3727 3912 4357 325
Size: 1255 1977 17 153 185 445 1498
Fragments arranged by size:
1977 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 3776 5488
Size: 274 80 33 51 1443 129 1670 1712
Cuts at: 5488 96
Size: 138
Fragments arranged by size:
1712 1670 1443 274 138 129 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5490 5522 5490
Size: 32 5498
Tth111I GACn'n_nGTC
Cuts at: 1316 2480 1316
Size: 1164 4366
XbaI T'CTAG_A
Cuts at: 2866 2866
Size: 5530
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5530
XmaI C'CCGG_G
Cuts at: 1870 2855 1870
Size: 985 4545
XmnI GAAnn'nnTTC
Cuts at: 2235 3659 2235
Size: 1424 4106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3338 276
Size: 53 83 186 936 1804 2468
Fragments arranged by size:
2468 1804 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI