Restriction map for
pFN21AE5328
(Circular) (Six-base) MAPSORT of: pFN21AE5328.seq Check: 1015 from: 1 to: 5521
pFN21AE5328 5521 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2277 3584 4968 5236 1632
Size: 645 1307 1384 268 1917
Fragments arranged by size:
1917 1384 1307 645 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 4420 5397 1203
Size: 3217 977 1327
Fragments arranged by size:
3217 1327 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 2029 4250 2029
Size: 2221 3300
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5521
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2388 2420 2388
Size: 32 5489
AlwNI CAG_nnn'CTG
Cuts at: 2423 3264 4836 2423
Size: 841 1572 3108
Fragments arranged by size:
3108 1572 841
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2846 1870
Size: 73 146 757 4545
Fragments arranged by size:
4545 757 146 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2842 4297 618
Size: 325 217 627 261 794 1455 1842
Fragments arranged by size:
1842 1455 794 627 325 261 217
BanII G_rGCy'C
Cuts at: 1391 2703 2840 1391
Size: 1312 137 4072
Fragments arranged by size:
4072 1312 137
BbeI G_GCGC'C
Cuts at: 2052 2052
Size: 5521
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2266 928
Size: 971 367 4183
Fragments arranged by size:
4183 971 367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3712 3746 1903
Size: 34 1775 34 3678
Fragments arranged by size:
3678 1775 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BfrBI ATG'CAT
Cuts at: 2732 2732
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4132 136
Size: 107 122 71 631 605 2460 1525
Fragments arranged by size:
2460 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2938 2938
Size: 5521
Bme1580I G_kGCm'C
Cuts at: 1790 3582 4738 1790
Size: 1792 1156 2573
Fragments arranged by size:
2573 1792 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3252 4210 442
Size: 416 53 83 399 24 1835 958 1753
Fragments arranged by size:
1835 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4181 1239
Size: 2942 2579
Bpu10I CC'TnA_GC
Cuts at: 2697 2762 2697
Size: 65 5456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2535 4184 882
Size: 617 1036 1649 2219
Fragments arranged by size:
2219 1649 1036 617
BsaAI yAC'GTr
Cuts at: 493 1204 3312 493
Size: 711 2108 2702
Fragments arranged by size:
2702 2108 711
BsaBI GATnn'nnATC
Cuts at: 2611 5515 2611
Size: 2904 2617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 3328 3710 4345
Size: 53 83 186 936 516 1279 382 635
Cuts at: 4345 275
Size: 1451
Fragments arranged by size:
1451 1279 936 635 516 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3954 4352 4626 4773 5184 3954
Size: 398 274 147 411 4291
Fragments arranged by size:
4291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5296
Fragments arranged by size:
5296 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2622 1186
Size: 1436 4085
BseYI C'CCAG_C
Cuts at: 2132 2683 4724 5220 2132
Size: 551 2041 496 2433
Fragments arranged by size:
2433 2041 551 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2043 1163
Size: 573 307 4641
Fragments arranged by size:
4641 573 307
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2026 2971 3732 4760 1540
Size: 275 186 25 945 761 1028 2301
Fragments arranged by size:
2301 1028 945 761 275 186 25
BsiHKAI G_wGCw'C
Cuts at: 2103 2306 2373 3582 3667 4738 2103
Size: 203 67 1209 85 1071 2886
Fragments arranged by size:
2886 1209 1071 203 85 67
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2306 2373 2703 2840 3582 3667
Size: 399 313 203 67 330 137 742 85
Cuts at: 3667 4738 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 330 313 203 137
85 67
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 2407 3410 5140 2407
Size: 1003 1730 2788
Fragments arranged by size:
2788 1730 1003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2876 844
Size: 2032 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3408 1394
Size: 429 1585 3507
Fragments arranged by size:
3507 1585 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4165 4352 1700
Size: 110 2355 187 2869
Fragments arranged by size:
2869 2355 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5521
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5521
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 2428 1184
Size: 1244 4277
BstYI r'GATC_y
Cuts at: 1972 2851 3218 3603 3620 5072 5158 5170 5516
Size: 879 367 385 17 1452 86 12 346
Cuts at: 5516 1972
Size: 1977
Fragments arranged by size:
1977 1452 879 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2361 2361
Size: 5521
BtgI C'CryG_G
Cuts at: 513 1065 2004 2044 513
Size: 552 939 40 3990
Fragments arranged by size:
3990 939 552 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2759 487
Size: 683 56 1533 3249
Fragments arranged by size:
3249 1533 683 56
BtsI GCAGTG_nn'
Cuts at: 3037 3831 3851 3037
Size: 794 20 4707
Fragments arranged by size:
4707 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2502 2825 2502
Size: 323 5198
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2968 3857
Size: 54 1425 50 275 18 250 888 889
Cuts at: 3857 5486 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 888 275 250 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2968 1537
Size: 275 1156 4090
Fragments arranged by size:
4090 1156 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3451 1353
Size: 2098 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4108 4482 4628 387
Size: 1064 138 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 3584 4181 4968 5236 1239
Size: 393 645 1307 597 787 268 1524
Fragments arranged by size:
1524 1307 787 645 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 5521
EcoO109I rG'GnC_Cy
Cuts at: 2286 2564 2286
Size: 278 5243
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2290 2669 2290
Size: 379 5142
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 2044 2052 4668 1678
Size: 366 8 2616 2531
Fragments arranged by size:
2616 2531 366 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2168 2200 4162
Size: 32 135 32 443 32 121 32 1962
Cuts at: 4162 4194 4236 4268 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1962 443 135 121 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KasI G'GCGC_C
Cuts at: 2048 2048
Size: 5521
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 2296 3120 2296
Size: 824 4697
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3699
Fragments arranged by size:
3699 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2727 3479 3838 3997 518
Size: 648 1561 752 359 159 2042
Fragments arranged by size:
2042 1561 752 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2250 2937 3614 4762 5007 1606
Size: 644 687 677 1148 245 2120
Fragments arranged by size:
2120 1148 687 677 644 245
NarI GG'CG_CC
Cuts at: 2049 2049
Size: 5521
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4030
Fragments arranged by size:
4030 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NsiI A_TGCA'T
Cuts at: 2734 2734
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2165 2561 1348
Size: 817 396 4308
Fragments arranged by size:
4308 817 396
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PfoI T'CCnGG_A
Cuts at: 2349 2349
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2286 2286
Size: 5521
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PstI C_TGCA'G
Cuts at: 830 2873 830
Size: 2043 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SfcI C'TryA_G
Cuts at: 826 1045 2869 4004 4685 4876 826
Size: 219 1824 1135 681 191 1471
Fragments arranged by size:
1824 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2050 2050
Size: 5521
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2091 2848 1872
Size: 219 757 4545
Fragments arranged by size:
4545 757 219
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3625 4526 4788 820
Size: 197 926 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 2671 3445 5
Size: 47 2619 774 2081
Fragments arranged by size:
2619 2081 774 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2960 513
Size: 552 939 48 908 3074
Fragments arranged by size:
3074 939 908 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2635 3548 3565 3718 3903 4348 325
Size: 1255 1055 913 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1055 913 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3767 5479 96
Size: 274 80 33 51 1443 1790 1712 138
Fragments arranged by size:
1790 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2296 2328 2384 2416 5481 5513 2296
Size: 32 56 32 3065 32 2304
Fragments arranged by size:
3065 2304 56 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2089 2846 1870
Size: 219 757 4545
Fragments arranged by size:
4545 757 219
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI