Restriction map for
pFN21AE5326
(Circular) (Six-base) MAPSORT of: pFN21AE5326.seq Check: 4538 from: 1 to: 5539
pFN21AE5326 5539 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2601 2882 1937
Size: 664 281 4594
Fragments arranged by size:
4594 664 281
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 2235 3666 4039 1563
Size: 672 1431 373 3063
Fragments arranged by size:
3063 1431 672 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 3602 4986 5254 1632
Size: 567 1403 1384 268 1917
Fragments arranged by size:
1917 1403 1384 567 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 2604 4438 5415 1203
Size: 1401 1834 977 1327
Fragments arranged by size:
1834 1401 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5539
AlwNI CAG_nnn'CTG
Cuts at: 2378 2431 3282 4854 2378
Size: 53 851 1572 3063
Fragments arranged by size:
3063 1572 851 53
ApaLI G'TGCA_C
Cuts at: 3596 4752 3596
Size: 1156 4383
ApoI r'AATT_y
Cuts at: 1493 2848 3074 3226 5335 1493
Size: 1355 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2864 1870
Size: 73 921 4545
Fragments arranged by size:
4545 921 73
BamHI G'GATC_C
Cuts at: 2869 3236 2869
Size: 367 5172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2860 4315 618
Size: 325 217 627 1073 1455 1842
Fragments arranged by size:
1842 1455 1073 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2858 1391
Size: 1467 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2187 2487 928
Size: 971 288 300 3980
Fragments arranged by size:
3980 971 300 288
BbvCI CC'TCA_GC
Cuts at: 2432 2432
Size: 5539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 1903
Size: 34 1793 34 3678
Fragments arranged by size:
3678 1793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5534 5534
Size: 5539
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 3600 4756 1790
Size: 1810 1156 2573
Fragments arranged by size:
2573 1810 1156
BmgBI CAC'GTC
Cuts at: 1318 2605 1318
Size: 1287 4252
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 4228 442
Size: 416 53 83 399 24 1853 958 1753
Fragments arranged by size:
1853 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2399 2630 4199 1239
Size: 1160 231 1569 2579
Fragments arranged by size:
2579 1569 1160 231
Bpu10I CC'TnA_GC
Cuts at: 2432 2432
Size: 5539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2751 3664 4529 4827 2751
Size: 913 865 298 3463
Fragments arranged by size:
3463 913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2334 2765 4202 882
Size: 617 835 431 1437 2219
Fragments arranged by size:
2219 1437 835 617 431
BsaAI yAC'GTr
Cuts at: 493 1204 3330 493
Size: 711 2126 2702
Fragments arranged by size:
2702 2126 711
BsaBI GATnn'nnATC
Cuts at: 5533 5533
Size: 5539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3346 3728 4363 275
Size: 53 83 186 936 1813 382 635 1451
Fragments arranged by size:
1813 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2639 2759 1186
Size: 1453 120 3966
Fragments arranged by size:
3966 1453 120
BseYI C'CCAG_C
Cuts at: 4742 5238 4742
Size: 496 5043
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4966
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2989 3750 4778 1540
Size: 275 186 988 761 1028 2301
Fragments arranged by size:
2301 1028 988 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3600 3685 4756 3600
Size: 85 1071 4383
Fragments arranged by size:
4383 1071 85
BsmI GAATG_Cn'
Cuts at: 3050 3143 3050
Size: 93 5446
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2858 3600 3685 4756 1391
Size: 399 1068 742 85 1071 2174
Fragments arranged by size:
2174 1071 1068 742 399 85
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 2394 3428 5158 2394
Size: 1034 1730 2775
Fragments arranged by size:
2775 1730 1034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 2386 2894 844
Size: 1508 34 508 3489
Fragments arranged by size:
3489 1508 508 34
BsrBI CCG'CTC
Cuts at: 1394 1823 2422 3426 1394
Size: 429 599 1004 3507
Fragments arranged by size:
3507 1004 599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2293 4034 4208 65
Size: 1183 1045 1741 174 1396
Fragments arranged by size:
1741 1396 1183 1045 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4183 4370 1700
Size: 110 2373 187 2869
Fragments arranged by size:
2869 2373 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5539
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5539
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2409 2869 3236 3621 3638 5090 5176 5188
Size: 437 460 367 385 17 1452 86 12
Cuts at: 5188 5534 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 460 437 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2803 513
Size: 552 939 799 3249
Fragments arranged by size:
3249 939 799 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 3055 3849 3869 2552
Size: 503 794 20 4222
Fragments arranged by size:
4222 794 503 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504
Size: 54 1425 50 275 18 1156 889 1629
Cuts at: 5504 8
Size: 43
Fragments arranged by size:
1629 1425 1156 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2986 1537
Size: 275 1174 4090
Fragments arranged by size:
4090 1174 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3469 1353
Size: 2116 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4126 4500 4646 387
Size: 1064 138 2537 374 146 1280
Fragments arranged by size:
2537 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2399 2630 3602 4199 4986 5254
Size: 393 567 200 231 972 597 787 268
Cuts at: 5254 1239
Size: 1524
Fragments arranged by size:
1524 972 787 597 567 393 268 231
200
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 2477
Size: 5539
EcoRI G'AATT_C
Cuts at: 2848 3226 2848
Size: 378 5161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2805 2805
Size: 5539
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 4686 1678
Size: 3008 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2394 2426 2620 2652 2804
Size: 32 135 32 822 32 194 32 152
Cuts at: 2804 2836 4180 4212 4254 4286 1373
Size: 32 1344 32 42 32 2626
Fragments arranged by size:
2626 1344 822 194 152 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2031 2883 3129 669
Size: 988 281 93 852 246 3079
Fragments arranged by size:
3079 988 852 281 246 93
HindIII A'AGCT_T
Cuts at: 748 2706 748
Size: 1958 3581
HpaI GTT'AAC
Cuts at: 2031 3129 2031
Size: 1098 4441
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2218 4653 4837 1305
Size: 913 2435 184 2007
Fragments arranged by size:
2435 2007 913 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2808 3497 3856 4015 518
Size: 648 1642 689 359 159 2042
Fragments arranged by size:
2042 1642 689 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2177 2955 3632 4780 5025 1606
Size: 571 778 677 1148 245 2120
Fragments arranged by size:
2120 1148 778 677 571 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2803 513
Size: 552 939 799 3249
Fragments arranged by size:
3249 939 799 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2305 2897 4442 1132
Size: 97 1076 592 1545 2229
Fragments arranged by size:
2229 1545 1076 592 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5539
PfoI T'CCnGG_A
Cuts at: 2415 2415
Size: 5539
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2383 2891 830
Size: 1553 508 3478
Fragments arranged by size:
3478 1553 508
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SfcI C'TryA_G
Cuts at: 826 1045 2379 2887 4022 4703 4894 826
Size: 219 1334 508 1135 681 191 1471
Fragments arranged by size:
1471 1334 1135 681 508 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2866 1872
Size: 994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2766 3643 4544 4806 820
Size: 197 926 823 877 901 262 1553
Fragments arranged by size:
1553 926 901 877 823 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 2503 3463 5
Size: 47 2451 960 2081
Fragments arranged by size:
2451 2081 960 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5539
StyI C'CwwG_G
Cuts at: 513 1065 2004 2803 2978 513
Size: 552 939 799 175 3074
Fragments arranged by size:
3074 939 799 552 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2201 3566 3583 3736 3921 4366 325
Size: 1255 621 1365 17 153 185 445 1498
Fragments arranged by size:
1498 1365 1255 621 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3785 5497 96
Size: 274 80 33 51 1443 1808 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5539
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2654 2654
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2864 1870
Size: 994 4545
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI