Restriction map for
pFN21AE5324
(Circular) (Six-base) MAPSORT of: pFN21AE5324.seq Check: 7767 from: 1 to: 5602
pFN21AE5324 5602 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2591 2945 1937
Size: 654 354 4594
Fragments arranged by size:
4594 654 354
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 2687 3665 5049 5317 1632
Size: 746 309 978 1384 268 1917
Fragments arranged by size:
1917 1384 978 746 309 268
AfeI AGC'GCT
Cuts at: 2014 2014
Size: 5602
AflII C'TTAA_G
Cuts at: 820 1017 2200 820
Size: 197 1183 4222
Fragments arranged by size:
4222 1183 197
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AlwNI CAG_nnn'CTG
Cuts at: 2185 2506 3345 4917 2185
Size: 321 839 1572 2870
Fragments arranged by size:
2870 1572 839 321
ApaI G_GGCC'C
Cuts at: 2900 2900
Size: 5602
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2051 2117 2911 3137 3289 5398 1493
Size: 558 66 794 226 152 2109 1697
Fragments arranged by size:
2109 1697 794 558 226 152 66
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 1870
Size: 73 984 4545
Fragments arranged by size:
4545 984 73
AvrII C'CTAG_G
Cuts at: 2020 2834 2020
Size: 814 4788
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2388 2923 4378 618
Size: 325 217 627 601 535 1455 1842
Fragments arranged by size:
1842 1455 627 601 535 325 217
BanII G_rGCy'C
Cuts at: 1391 2900 2921 1391
Size: 1509 21 4072
Fragments arranged by size:
4072 1509 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 2758 2876 928
Size: 971 400 459 118 3654
Fragments arranged by size:
3654 971 459 400 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3793 3827 1903
Size: 34 50 34 1772 34 3678
Fragments arranged by size:
3678 1772 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2493 3494 4704 2493
Size: 1001 1210 3391
Fragments arranged by size:
3391 1210 1001
BclI T'GATC_A
Cuts at: 1548 2187 2820 1548
Size: 639 633 4330
Fragments arranged by size:
4330 639 633
BfrBI ATG'CAT
Cuts at: 2513 2513
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2160 2397 4213
Size: 107 122 71 631 605 488 237 1816
Cuts at: 4213 136
Size: 1525
Fragments arranged by size:
1816 1525 631 605 488 237 122 107
71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 2009 3019 2009
Size: 1010 4592
Bme1580I G_kGCm'C
Cuts at: 1790 2900 3663 4819 1790
Size: 1110 763 1156 2573
Fragments arranged by size:
2573 1156 1110 763
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2894 3333 4291
Size: 416 53 83 399 24 1477 439 958
Cuts at: 4291 442
Size: 1753
Fragments arranged by size:
1753 1477 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2113 4262 1239
Size: 874 2149 2579
Fragments arranged by size:
2579 2149 874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 3791 4426 275
Size: 53 83 186 936 1876 382 635 1451
Fragments arranged by size:
1876 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2731 2761 1178
Size: 30 165 30 1328 30 4019
Fragments arranged by size:
4019 1328 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5602
BseYI C'CCAG_C
Cuts at: 2892 4805 5301 2892
Size: 1913 496 3193
Fragments arranged by size:
3193 1913 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5029
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2338 3052 3813 4841 1540
Size: 275 186 337 714 761 1028 2301
Fragments arranged by size:
2301 1028 761 714 337 275 186
BsiHKAI G_wGCw'C
Cuts at: 2884 3663 3748 4819 2884
Size: 779 85 1071 3667
Fragments arranged by size:
3667 1071 779 85
BsmI GAATG_Cn'
Cuts at: 2475 3113 3206 2475
Size: 638 93 4871
Fragments arranged by size:
4871 638 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2884 2900 2921 3663 3748 4819 1391
Size: 399 1094 16 21 742 85 1071 2174
Fragments arranged by size:
2174 1094 1071 742 399 85 21 16
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 844
Size: 2113 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstXI CCAn_nnnn'nTGG
Cuts at: 2624 2624
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2859 2932 3299 3684 3701 5153 5239 5251
Size: 887 73 367 385 17 1452 86 12
Cuts at: 5251 5597 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 887 385 367 346 86 73
17 12
BstZ17I GTA'TAC
Cuts at: 2592 2592
Size: 5602
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5050
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 2049 2186 2658 3118 3912 3932 2049
Size: 137 472 460 794 20 3719
Fragments arranged by size:
3719 794 472 460 137 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2712 3049 3938
Size: 54 1425 50 275 18 882 337 889
Cuts at: 3938 5567 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 882 337 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2808 3532 1353
Size: 1455 724 3423
Fragments arranged by size:
3423 1455 724
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2113 2378 2687 3665 4262 5049 5317
Size: 393 481 265 309 978 597 787 268
Cuts at: 5317 1239
Size: 1524
Fragments arranged by size:
1524 978 787 597 481 393 309 268
265
EcoNI CCTnn'n_nnAGG
Cuts at: 2460 2460
Size: 5602
EcoO109I rG'GnC_Cy
Cuts at: 2260 2367 2896 2260
Size: 107 529 4966
Fragments arranged by size:
4966 529 107
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2016 4749 1678
Size: 338 2733 2531
Fragments arranged by size:
2733 2531 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2094 2126 2320 2352 2731
Size: 32 135 32 522 32 194 32 379
Cuts at: 2731 2763 4243 4275 4317 4349 1373
Size: 32 1480 32 42 32 2626
Fragments arranged by size:
2626 1480 522 379 194 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2203 748
Size: 1455 4147
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2714 10
Size: 54 1425 343 882 2898
Fragments arranged by size:
2898 1425 882 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3560 3919 4078 518
Size: 648 2394 359 159 2042
Fragments arranged by size:
2394 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2624 3018 3695 4843 5088 1606
Size: 1018 394 677 1148 245 2120
Fragments arranged by size:
2120 1148 1018 677 394 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5050
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NsiI A_TGCA'T
Cuts at: 2515 2515
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2960 4505 1132
Size: 97 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2283 1108
Size: 1175 4427
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 2260
Size: 5602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 2408 3172 2408
Size: 764 4838
PspOMI G'GGCC_C
Cuts at: 2896 2896
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2181 2954 830
Size: 1351 773 3478
Fragments arranged by size:
3478 1351 773
PvuI CG_AT'CG
Cuts at: 2001 2338 2001
Size: 337 5265
PvuII CAG'CTG
Cuts at: 2624 2624
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SexAI A'CCwGG_T
Cuts at: 2697 2697
Size: 5602
SfcI C'TryA_G
Cuts at: 826 1045 2177 2950 4085 4766 4957 826
Size: 219 1132 773 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 773 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2200 3706 4607 4869 820
Size: 197 926 257 1506 901 262 1553
Fragments arranged by size:
1553 1506 926 901 262 257 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2020 2461 2646 2673 2834 3041 513
Size: 552 955 441 185 27 161 207 3074
Fragments arranged by size:
3074 955 552 441 207 185 161 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2070 2102 5562 5594 2070
Size: 32 3460 32 2078
Fragments arranged by size:
3460 2078 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI