Restriction map for
pFN21AE5318
(Circular) (Six-base) MAPSORT of: pFN21AE5318.seq Check: 979 from: 1 to: 5563
pFN21AE5318 5563 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 278
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2906 1937
Size: 969 4594
Acc65I G'GTAC_C
Cuts at: 2858 2884 2858
Size: 26 5537
AclI AA'CG_TT
Cuts at: 1563 3690 4063 1563
Size: 2127 373 3063
Fragments arranged by size:
3063 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3626 5010 5278 1632
Size: 1994 1384 268 1917
Fragments arranged by size:
1994 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5366
AflIII A'CryG_T
Cuts at: 1203 4462 5439 1203
Size: 3259 977 1327
Fragments arranged by size:
3259 1327 977
AgeI A'CCGG_T
Cuts at: 4394 4394
Size: 5563
AhdI GACnn_n'nnGTC
Cuts at: 4292 4292
Size: 5563
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5563
AlwNI CAG_nnn'CTG
Cuts at: 2443 2641 2713 3306 4878 2443
Size: 198 72 593 1572 3128
Fragments arranged by size:
3128 1572 593 198 72
ApaLI G'TGCA_C
Cuts at: 2722 3620 4776 2722
Size: 898 1156 3509
Fragments arranged by size:
3509 1156 898
ApoI r'AATT_y
Cuts at: 1493 2872 3098 3250 5359 1493
Size: 1379 226 152 2109 1697
Fragments arranged by size:
2109 1697 1379 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4117 160
Size: 872 3085 1606
Fragments arranged by size:
3085 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
AvaI C'yCGr_G
Cuts at: 1870 1943 2083 2379 2888 1870
Size: 73 140 296 509 4545
Fragments arranged by size:
4545 509 296 140 73
BamHI G'GATC_C
Cuts at: 2893 3260 2893
Size: 367 5196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2858 2884 4339 618
Size: 325 217 627 291 780 26 1455 1842
Fragments arranged by size:
1842 1455 780 627 325 291 217 26
BanII G_rGCy'C
Cuts at: 1391 2093 2234 2472 2882 1391
Size: 702 141 238 410 4072
Fragments arranged by size:
4072 702 410 238 141
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2817 2851 3754 3788 1903
Size: 34 880 34 903 34 3678
Fragments arranged by size:
3678 903 880 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 4665 3455
Size: 1210 4353
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2238 2622
Size: 107 122 71 631 605 337 229 384
Cuts at: 2622 4174 136
Size: 1552 1525
Fragments arranged by size:
1552 1525 631 605 384 337 229 122
107 71
BglII A'GATC_T
Cuts at: 2173 5558 2173
Size: 3385 2178
BlpI GC'TnA_GC
Cuts at: 2245 2327 2980 2245
Size: 82 653 4828
Fragments arranged by size:
4828 653 82
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2487 2726 3624 4780 1790
Size: 293 404 239 898 1156 2573
Fragments arranged by size:
2573 1156 898 404 293 239
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2431 3294 4252
Size: 416 53 83 399 24 1014 863 958
Cuts at: 4252 442
Size: 1753
Fragments arranged by size:
1753 1014 958 863 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5563
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 5563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2342 2678 2684 4223 1239
Size: 839 264 336 6 1539 2579
Fragments arranged by size:
2579 1539 839 336 264 6
Bpu10I CC'TnA_GC
Cuts at: 2106 2106
Size: 5563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3688 4553 4851 3688
Size: 865 298 4400
Fragments arranged by size:
4400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4226 882
Size: 617 2727 2219
Fragments arranged by size:
2727 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3354 493
Size: 711 2150 2702
Fragments arranged by size:
2702 2150 711
BsaBI GATnn'nnATC
Cuts at: 2172 5557 2172
Size: 3385 2178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3370 3752 4387 275
Size: 53 83 186 936 1837 382 635 1451
Fragments arranged by size:
1837 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2781 3996 4394 4668 4815 5226 2781
Size: 1215 398 274 147 411 3118
Fragments arranged by size:
3118 1215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5338
Fragments arranged by size:
5338 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2480 2702 2705 2768 2864 1186
Size: 889 405 222 3 63 96 3885
Fragments arranged by size:
3885 889 405 222 96 63 3
BseYI C'CCAG_C
Cuts at: 2180 2331 4766 5262 2180
Size: 151 2435 496 2481
Fragments arranged by size:
2481 2435 496 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4990
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3774 4802 1540
Size: 275 186 1012 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2726 3624 3709 4780 2726
Size: 898 85 1071 3509
Fragments arranged by size:
3509 1071 898 85
BsmI GAATG_Cn'
Cuts at: 3074 3167 3074
Size: 93 5470
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2093 2234 2472 2487 2726 2882
Size: 399 293 10 141 238 15 239 156
Cuts at: 2882 3624 3709 4780 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 293 239 238 156
141 85 15 10
BspEI T'CCGG_A
Cuts at: 2781 5226 2781
Size: 2445 3118
BspHI T'CATG_A
Cuts at: 2113 3452 5182 2113
Size: 1339 1730 2494
Fragments arranged by size:
2494 1730 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2918 844
Size: 2074 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3450 1394
Size: 429 1627 3507
Fragments arranged by size:
3507 1627 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4058 4232 65
Size: 1183 2810 174 1396
Fragments arranged by size:
2810 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2726 4207 4394 1700
Size: 110 916 1481 187 2869
Fragments arranged by size:
2869 1481 916 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5563
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5563
BssSI C'ACGA_G
Cuts at: 3623 4635 3623
Size: 1012 4551
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2713 794
Size: 1919 3644
BstBI TT'CG_AA
Cuts at: 4367 4367
Size: 5563
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5563
BstYI r'GATC_y
Cuts at: 1972 2173 2493 2893 3260 3645 3662 5114 5200
Size: 201 320 400 367 385 17 1452 86
Cuts at: 5200 5212 5558 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 400 385 367 346 320 201
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2144 2415 2488 513
Size: 552 1079 271 73 3588
Fragments arranged by size:
3588 1079 552 271 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4824
Fragments arranged by size:
4824 683 56
BtsI GCAGTG_nn'
Cuts at: 3079 3873 3893 3079
Size: 794 20 4749
Fragments arranged by size:
4749 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5528
DraI TTT'AAA
Cuts at: 2867 2867
Size: 5563
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4570 809
Size: 507 3254 1802
Fragments arranged by size:
3254 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2285 2728
Size: 54 1425 50 275 18 180 275 443
Cuts at: 2728 3010 3899 5528 8
Size: 282 889 1629 43
Fragments arranged by size:
1629 1425 889 443 282 275 275 180
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 1537
Size: 275 1198 4090
Fragments arranged by size:
4090 1198 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2385 3493 1353
Size: 1032 1108 3423
Fragments arranged by size:
3423 1108 1032
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2699 4150 4524 4670 387
Size: 1064 138 1110 1451 374 146 1280
Fragments arranged by size:
1451 1280 1110 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2342 2678 2684 3626 4223 5010
Size: 393 446 264 336 6 942 597 787
Cuts at: 5010 5278 1239
Size: 268 1524
Fragments arranged by size:
1524 942 787 597 446 393 336 268
264 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 5563
EcoO109I rG'GnC_Cy
Cuts at: 2373 2458 2373
Size: 85 5478
EcoRI G'AATT_C
Cuts at: 2872 3250 2872
Size: 378 5185
FspI TGC'GCA
Cuts at: 4069 4069
Size: 5563
HaeII r_GCGC'y
Cuts at: 1678 4710 1678
Size: 3032 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 4204 4236 4278
Size: 32 135 32 427 32 2173 32 42
Cuts at: 4278 4310 1373
Size: 32 2626
Fragments arranged by size:
2626 2173 427 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2907 3153 669
Size: 988 281 969 246 3079
Fragments arranged by size:
3079 988 969 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5563
HpaI GTT'AAC
Cuts at: 3153 3153
Size: 5563
KpnI G_GTAC'C
Cuts at: 2862 2888 2862
Size: 26 5537
MfeI C'AATT_G
Cuts at: 3162 3162
Size: 5563
MluI A'CGCG_T
Cuts at: 5439 5439
Size: 5563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4677 4861 1305
Size: 3372 184 2007
Fragments arranged by size:
3372 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 10
Size: 54 1425 343 180 3561
Fragments arranged by size:
3561 1425 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3521 3880 4039 518
Size: 648 2355 359 159 2042
Fragments arranged by size:
2355 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2249 2350 2572 2713 2797 2841 2979
Size: 540 103 101 222 141 84 44 138
Cuts at: 2979 3656 4804 5049 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 245 222 141 138
103 101 84 44
NcoI C'CATG_G
Cuts at: 513 1065 2488 513
Size: 552 1423 3588
Fragments arranged by size:
3588 1423 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5563
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5563
NotI GC'GGCC_GC
Cuts at: 3010 3010
Size: 5563
NspI r_CATG'y
Cuts at: 1132 1229 2921 4466 1132
Size: 97 1692 1545 2229
Fragments arranged by size:
2229 1692 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2234 1348
Size: 886 4677
PciI A'CATG_T
Cuts at: 4462 4462
Size: 5563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2416 1108
Size: 1308 4255
PmeI GTTT'AAAC
Cuts at: 2867 2867
Size: 5563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 5145 5177 3632
Size: 32 1481 32 4018
Fragments arranged by size:
4018 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2458 2458
Size: 5563
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5563
PsiI TTA'TAA
Cuts at: 3133 3133
Size: 5563
PstI C_TGCA'G
Cuts at: 830 2622 2709 2718 2915 830
Size: 1792 87 9 197 3478
Fragments arranged by size:
3478 1792 197 87 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5563
PvuII CAG'CTG
Cuts at: 2713 2713
Size: 5563
RsrII CG'GwC_CG
Cuts at: 2784 2784
Size: 5563
SacII CC_GC'GG
Cuts at: 2147 2147
Size: 5563
SalI G'TCGA_C
Cuts at: 1936 2905 1936
Size: 969 4594
SanDI GG'GwC_CC
Cuts at: 2458 2458
Size: 5563
SbfI CC_TGCA'GG
Cuts at: 2622 2915 2622
Size: 293 5270
ScaI AGT'ACT
Cuts at: 3811 5523 3811
Size: 1712 3851
SfcI C'TryA_G
Cuts at: 826 1045 2618 2705 2714 2911 4046 4727 4918
Size: 219 1573 87 9 197 1135 681 191
Cuts at: 4918 826
Size: 1471
Fragments arranged by size:
1573 1471 1135 681 219 197 191 87
9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5563
SmaI CCC'GGG
Cuts at: 1872 2085 2381 2890 1872
Size: 213 296 509 4545
Fragments arranged by size:
4545 509 296 213
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3667 4568 4830 820
Size: 197 926 1724 901 262 1553
Fragments arranged by size:
1724 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3354 493
Size: 2861 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5563
SphI G_CATG'C
Cuts at: 1132 2921 1132
Size: 1789 3774
SspI AAT'ATT
Cuts at: 5 52 3487 5
Size: 47 3435 2081
Fragments arranged by size:
3435 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5563
StyI C'CwwG_G
Cuts at: 513 1065 2488 3002 513
Size: 552 1423 514 3074
Fragments arranged by size:
3074 1423 552 514
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 2517 2801 3590 3607 3760 3945
Size: 1255 870 67 284 789 17 153 185
Cuts at: 3945 4390 325
Size: 445 1498
Fragments arranged by size:
1498 1255 870 789 445 284 185 153
67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3809 5521 96
Size: 274 80 33 51 1443 1832 1712 138
Fragments arranged by size:
1832 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2492 2524 5523 5555 2492
Size: 32 2999 32 2500
Fragments arranged by size:
2999 2500 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5563
XbaI T'CTAG_A
Cuts at: 2899 2899
Size: 5563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5563
XmaI C'CCGG_G
Cuts at: 1870 2083 2379 2888 1870
Size: 213 296 509 4545
Fragments arranged by size:
4545 509 296 213
XmnI GAAnn'nnTTC
Cuts at: 2579 3692 2579
Size: 1113 4450
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 276
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI SacI SapI SexAI SfiI SfoI SrfI SwaI
XcmI