Restriction map for
pFN21AE5316
(Circular) (Six-base) MAPSORT of: pFN21AE5316.seq Check: 1302 from: 1 to: 5560
pFN21AE5316 5560 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2395 3370 278
Size: 53 83 186 936 859 975 2468
Fragments arranged by size:
2468 975 936 859 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2903 1937
Size: 966 4594
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2121 2190 2484 3623 5007 5275 1632
Size: 489 69 294 1139 1384 268 1917
Fragments arranged by size:
1917 1384 1139 489 294 268 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5363
AflIII A'CryG_T
Cuts at: 1203 4459 5436 1203
Size: 3256 977 1327
Fragments arranged by size:
3256 1327 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 2111 2317 3303 4875 2111
Size: 206 986 1572 2796
Fragments arranged by size:
2796 1572 986 206
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2869 3095 3247 5356 1493
Size: 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 1870
Size: 73 942 4545
Fragments arranged by size:
4545 942 73
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 2881 4336 618
Size: 325 217 627 440 654 1455 1842
Fragments arranged by size:
1842 1455 654 627 440 325 217
BanII G_rGCy'C
Cuts at: 1391 2364 2476 2879 1391
Size: 973 112 403 4072
Fragments arranged by size:
4072 973 403 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2579 928
Size: 971 680 3909
Fragments arranged by size:
3909 971 680
BbvCI CC'TCA_GC
Cuts at: 2508 2681 2508
Size: 173 5387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BfrBI ATG'CAT
Cuts at: 2551 2551
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 136
Size: 107 122 71 631 605 2499 1525
Fragments arranged by size:
2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 3621 4777 1790
Size: 1831 1156 2573
Fragments arranged by size:
2573 1831 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4220 1239
Size: 2981 2579
Bpu10I CC'TnA_GC
Cuts at: 2508 2681 2508
Size: 173 5387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 2117 4223 882
Size: 617 609 9 2106 2219
Fragments arranged by size:
2219 2106 617 609 9
BsaAI yAC'GTr
Cuts at: 493 1204 3351 493
Size: 711 2147 2702
Fragments arranged by size:
2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2392 3367 3749 4384
Size: 53 83 186 936 859 975 382 635
Cuts at: 4384 275
Size: 1451
Fragments arranged by size:
1451 975 936 859 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2277 2424 3993 4391 4665 4812 5223 2277
Size: 147 1569 398 274 147 411 2614
Fragments arranged by size:
2614 1569 411 398 274 147 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2241 2271 1178
Size: 30 165 30 838 30 4467
Fragments arranged by size:
4467 838 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2057 2060 2093 2135 2201 2366 2369
Size: 868 3 3 33 42 66 165 3
Cuts at: 2369 2372 2498 2501 2504 2653 1186
Size: 3 126 3 3 149 4093
Fragments arranged by size:
4093 868 165 149 126 66 42 33
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 4763 5259 4763
Size: 496 5064
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4987
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 3621 3706 4777 2364
Size: 1257 85 1071 3147
Fragments arranged by size:
3147 1257 1071 85
BsmI GAATG_Cn'
Cuts at: 3071 3164 3071
Size: 93 5467
BsmBI CGTCTCn'nnnn_
Cuts at: 2383 2383
Size: 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2476 2879 3621 3706 4777 1391
Size: 399 574 112 403 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 574 403 399 112 85
BspEI T'CCGG_A
Cuts at: 2424 5223 2424
Size: 2799 2761
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2751 2915 844
Size: 1907 164 3489
Fragments arranged by size:
3489 1907 164
BsrBI CCG'CTC
Cuts at: 1394 1823 2404 2434 2446 2581 2608 3447 1394
Size: 429 581 30 12 135 27 839 3507
Fragments arranged by size:
3507 839 581 429 135 30 27 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 4055 4229 65
Size: 1183 2807 174 1396
Fragments arranged by size:
2807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4204 4391 1700
Size: 110 2394 187 2869
Fragments arranged by size:
2869 2394 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5560
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2238 2890 3257 3642 3659 5111 5197 5209
Size: 266 652 367 385 17 1452 86 12
Cuts at: 5209 5555 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 652 385 367 346 266 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2827 513
Size: 552 1762 3246
Fragments arranged by size:
3246 1762 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 3076 3870 3890 3076
Size: 794 20 4746
Fragments arranged by size:
4746 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2652 3007 3896
Size: 54 1425 50 275 18 822 355 889
Cuts at: 3896 5525 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 822 355 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 2025 2064 2085 2145 2460 2588 3490
Size: 660 12 39 21 60 315 128 902
Cuts at: 3490 1353
Size: 3423
Fragments arranged by size:
3423 902 660 315 128 60 39 21
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4147 4521 4667 387
Size: 1064 138 2558 374 146 1280
Fragments arranged by size:
2558 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2362 2362
Size: 5560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2190 2484 3623 4220 5007 5275
Size: 393 489 69 294 1139 597 787 268
Cuts at: 5275 1239
Size: 1524
Fragments arranged by size:
1524 1139 787 597 489 393 294 268
69
EcoO109I rG'GnC_Cy
Cuts at: 2296 2296
Size: 5560
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 2318 2854 4707 1678
Size: 640 536 1853 2531
Fragments arranged by size:
2531 1853 640 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2311 2343 4201 4233 4275
Size: 32 135 32 739 32 1858 32 42
Cuts at: 4275 4307 1373
Size: 32 2626
Fragments arranged by size:
2626 1858 739 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2904 3150 669
Size: 988 281 966 246 3079
Fragments arranged by size:
3079 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 4674 4858 1305
Size: 1260 2109 184 2007
Fragments arranged by size:
2109 2007 1260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2654 10
Size: 54 1425 343 822 2916
Fragments arranged by size:
2916 1425 822 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2667 3518 3877 4036 518
Size: 648 1501 851 359 159 2042
Fragments arranged by size:
2042 1501 851 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2087 2680 2829 2976 3653 4801 5046
Size: 457 24 593 149 147 677 1148 245
Cuts at: 5046 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 593 457 245 149 147
24
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5008
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NsiI A_TGCA'T
Cuts at: 2553 2553
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2918 4463 1132
Size: 97 1689 1545 2229
Fragments arranged by size:
2229 1689 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5560
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5560
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2296 2296
Size: 5560
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PstI C_TGCA'G
Cuts at: 830 2748 2912 830
Size: 1918 164 3478
Fragments arranged by size:
3478 1918 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
PvuII CAG'CTG
Cuts at: 2063 2680 2063
Size: 617 4943
SacI G_AGCT'C
Cuts at: 2364 2364
Size: 5560
SacII CC_GC'GG
Cuts at: 2830 2830
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SapI GCTCTTCn'nnn_
Cuts at: 2064 2064
Size: 5560
SbfI CC_TGCA'GG
Cuts at: 2748 2912 2748
Size: 164 5396
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SfcI C'TryA_G
Cuts at: 826 1045 2744 2908 4043 4724 4915 826
Size: 219 1699 164 1135 681 191 1471
Fragments arranged by size:
1699 1471 1135 681 219 191 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3664 4565 4827 820
Size: 197 926 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5560
StyI C'CwwG_G
Cuts at: 513 1065 2694 2999 513
Size: 552 1629 305 3074
Fragments arranged by size:
3074 1629 552 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2132 3587 3604 3757 3942 4387 325
Size: 1255 552 1455 17 153 185 445 1498
Fragments arranged by size:
1498 1455 1255 552 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3806 5518 96
Size: 274 80 33 51 1443 1829 1712 138
Fragments arranged by size:
1829 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2241 2273 5520 5552 2241
Size: 32 3247 32 2249
Fragments arranged by size:
3247 2249 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 3689 3689
Size: 5560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2393 3368 276
Size: 53 83 186 936 859 975 2468
Fragments arranged by size:
2468 975 936 859 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI