Restriction map for
pFN21AE5297
(Circular) (Six-base) MAPSORT of: pFN21AE5297.seq Check: 4728 from: 1 to: 8116
pFN21AE5297 8116 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5926 278
Size: 53 83 186 936 4390 2468
Fragments arranged by size:
4390 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2676 5459 1937
Size: 739 2783 4594
Fragments arranged by size:
4594 2783 739
Acc65I G'GTAC_C
Cuts at: 4407 5437 4407
Size: 1030 7086
AclI AA'CG_TT
Cuts at: 1563 2732 6243 6616 1563
Size: 1169 3511 373 3063
Fragments arranged by size:
3511 3063 1169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 2572 2706 2755 3176 3532 6179 7563
Size: 728 212 134 49 421 356 2647 1384
Cuts at: 7563 7831 1632
Size: 268 1917
Fragments arranged by size:
2647 1917 1384 728 421 356 268 212
134 49
AfeI AGC'GCT
Cuts at: 4230 4230
Size: 8116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7919
AflIII A'CryG_T
Cuts at: 1203 2640 2679 3996 4242 4261 7015 7992 1203
Size: 1437 39 1317 246 19 2754 977 1327
Fragments arranged by size:
2754 1437 1327 1317 977 246 39 19
AgeI A'CCGG_T
Cuts at: 6947 6947
Size: 8116
AhdI GACnn_n'nnGTC
Cuts at: 6845 6845
Size: 8116
AleI CACnn'nnGTG
Cuts at: 1166 2639 3530 4001 4514 4848 1166
Size: 1473 891 471 513 334 4434
Fragments arranged by size:
4434 1473 891 513 471 334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8116
AlwNI CAG_nnn'CTG
Cuts at: 2103 2434 3154 3514 4864 4918 5859 7431 2103
Size: 331 720 360 1350 54 941 1572 2788
Fragments arranged by size:
2788 1572 1350 941 720 360 331 54
ApaI G_GGCC'C
Cuts at: 5109 5109
Size: 8116
ApaLI G'TGCA_C
Cuts at: 3656 4639 6173 7329 3656
Size: 983 1534 1156 4443
Fragments arranged by size:
4443 1534 1156 983
ApoI r'AATT_y
Cuts at: 1493 2471 4580 4595 5425 5651 5803 7912 1493
Size: 978 2109 15 830 226 152 2109 1697
Fragments arranged by size:
2109 2109 1697 978 830 226 152 15
AseI AT'TA_AT
Cuts at: 160 1032 2985 6670 160
Size: 872 1953 3685 1606
Fragments arranged by size:
3685 1953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8116
AvaI C'yCGr_G
Cuts at: 1870 1943 3963 4290 4719 4834 5441 1870
Size: 73 2020 327 429 115 607 4545
Fragments arranged by size:
4545 2020 607 429 327 115 73
BamHI G'GATC_C
Cuts at: 3039 5446 5813 3039
Size: 2407 367 5342
Fragments arranged by size:
5342 2407 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2555 3221 3634 4286
Size: 325 217 627 396 372 666 413 652
Cuts at: 4286 4407 5336 5437 6892 618
Size: 121 929 101 1455 1842
Fragments arranged by size:
1842 1455 929 666 652 627 413 396
372 325 217 121 101
BanII G_rGCy'C
Cuts at: 1391 3602 4328 4955 5109 5435 1391
Size: 2211 726 627 154 326 4072
Fragments arranged by size:
4072 2211 726 627 326 154
BbeI G_GCGC'C
Cuts at: 2559 3225 3638 5340 2559
Size: 666 413 1702 5335
Fragments arranged by size:
5335 1702 666 413
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2156 2695 3538 3801 4438 4981 928
Size: 971 257 539 843 263 637 543 4063
Fragments arranged by size:
4063 971 843 637 543 539 263 257
BbvCI CC'TCA_GC
Cuts at: 2918 3494 5350 2918
Size: 576 1856 5684
Fragments arranged by size:
5684 1856 576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 6307 6341 1903
Size: 34 2363 34 1973 34 3678
Fragments arranged by size:
3678 2363 1973 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 6008 7218 3656
Size: 2352 1210 4554
Fragments arranged by size:
4554 2352 1210
BclI T'GATC_A
Cuts at: 1548 2514 3825 1548
Size: 966 1311 5839
Fragments arranged by size:
5839 1311 966
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2453 6727 136
Size: 107 122 71 631 605 781 4274 1525
Fragments arranged by size:
4274 1525 781 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3369 8111 3369
Size: 4742 3374
BlpI GC'TnA_GC
Cuts at: 3422 4100 4775 5533 3422
Size: 678 675 758 6005
Fragments arranged by size:
6005 758 678 675
Bme1580I G_kGCm'C
Cuts at: 1790 3660 4289 4643 5109 6177 7333 1790
Size: 1870 629 354 466 1068 1156 2573
Fragments arranged by size:
2573 1870 1156 1068 629 466 354
BmgBI CAC'GTC
Cuts at: 1318 2896 3999 1318
Size: 1578 1103 5435
Fragments arranged by size:
5435 1578 1103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5847 6805 442
Size: 416 53 83 399 24 4430 958 1753
Fragments arranged by size:
4430 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8116
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4354 4354
Size: 8116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2077 2099 2591 4172 4571 4955 6776 1239
Size: 838 22 492 1581 399 384 1821 2579
Fragments arranged by size:
2579 1821 1581 838 492 399 384 22
Bpu10I CC'TnA_GC
Cuts at: 2918 3494 5350 2918
Size: 576 1856 5684
Fragments arranged by size:
5684 1856 576
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6241 7106 7404 6241
Size: 865 298 6953
Fragments arranged by size:
6953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2718 4342 6779 882
Size: 617 1219 1624 2437 2219
Fragments arranged by size:
2437 2219 1624 1219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3418 4264 5907 493
Size: 711 2214 846 1643 2702
Fragments arranged by size:
2702 2214 1643 846 711
BsaBI GATnn'nnATC
Cuts at: 8110 8110
Size: 8116
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2556 3222 3635 4563
Size: 53 83 186 936 1023 666 413 928
Cuts at: 4563 5337 5923 6305 6940 275
Size: 774 586 382 635 1451
Fragments arranged by size:
1451 1023 936 928 774 666 635 586
413 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 5253 6549 6947 7221 7368 7779 4092
Size: 1161 1296 398 274 147 411 4429
Fragments arranged by size:
4429 1296 1161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 3683 3713 4169
Size: 30 165 30 672 30 1578 30 456
Cuts at: 4169 4199 1178
Size: 30 5095
Fragments arranged by size:
5095 1578 672 456 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2465 3107 3482 4178 4265 5144 5147
Size: 1177 102 642 375 696 87 879 3
Cuts at: 5147 5327 1186
Size: 180 3975
Fragments arranged by size:
3975 1177 879 696 642 375 180 102
87 3
BseYI C'CCAG_C
Cuts at: 2087 2551 4458 4767 4857 7319 7815 2087
Size: 464 1907 309 90 2462 496 2388
Fragments arranged by size:
2462 2388 1907 496 464 309 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 2645 3398 4639 4872 5150 1163
Size: 573 429 480 753 1241 233 278 4129
Fragments arranged by size:
4129 1241 753 573 480 429 278 233
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5566 6327 7355 1540
Size: 275 186 3565 761 1028 2301
Fragments arranged by size:
3565 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3423 3660 4643 6177 6262 7333 3423
Size: 237 983 1534 85 1071 4206
Fragments arranged by size:
4206 1534 1071 983 237 85
BsmI GAATG_Cn'
Cuts at: 2701 3579 5323 5627 5720 2701
Size: 878 1744 304 93 5097
Fragments arranged by size:
5097 1744 878 304 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4718 4718
Size: 8116
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3423 3602 3660 4289 4328 4643 4955
Size: 399 1633 179 58 629 39 315 312
Cuts at: 4955 5109 5435 6177 6262 7333 1391
Size: 154 326 742 85 1071 2174
Fragments arranged by size:
2174 1633 1071 742 629 399 326 315
312 179 154 85 58 39
BspEI T'CCGG_A
Cuts at: 7779 7779
Size: 8116
BspHI T'CATG_A
Cuts at: 6005 7735 6005
Size: 1730 6386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2604 3420 5471 844
Size: 1760 816 2051 3489
Fragments arranged by size:
3489 2051 1760 816
BsrBI CCG'CTC
Cuts at: 1394 1823 2901 6003 1394
Size: 429 1078 3102 3507
Fragments arranged by size:
3507 3102 1078 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4545 6611 6785 65
Size: 1183 3297 2066 174 1396
Fragments arranged by size:
3297 2066 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2599 3241 4049 5068 5277 6760 6947
Size: 110 789 642 808 1019 209 1483 187
Cuts at: 6947 1700
Size: 2869
Fragments arranged by size:
2869 1483 1019 808 789 642 209 187
110
BsrGI T'GTAC_A
Cuts at: 96 2643 3723 96
Size: 2547 1080 4489
Fragments arranged by size:
4489 2547 1080
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8116
BssSI C'ACGA_G
Cuts at: 6176 7188 6176
Size: 1012 7104
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4024 794
Size: 3230 4886
BstBI TT'CG_AA
Cuts at: 6920 6920
Size: 8116
BstEII G'GTnAC_C
Cuts at: 1184 4522 1184
Size: 3338 4778
BstXI CCAn_nnnn'nTGG
Cuts at: 2956 2956
Size: 8116
BstYI r'GATC_y
Cuts at: 1972 2775 3039 3150 3369 3772 4059 5446 5813
Size: 803 264 111 219 403 287 1387 367
Cuts at: 5813 6198 6215 7667 7753 7765 8111 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1387 803 403 385 367 346
287 264 219 111 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4362 4362
Size: 8116
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 2839 3225 3432 3574 3970
Size: 552 939 123 712 386 207 142 396
Cuts at: 3970 4209 4491 4527 4557 513
Size: 239 282 36 30 4072
Fragments arranged by size:
4072 939 712 552 396 386 282 239
207 142 123 36 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7377
Fragments arranged by size:
7377 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 3518 4622 5632 6426 6446 2761
Size: 757 1104 1010 794 20 4431
Fragments arranged by size:
4431 1104 1010 794 757 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2109 2144 529
Size: 35 1545 35 6501
Fragments arranged by size:
6501 1545 35 35
DraI TTT'AAA
Cuts at: 2667 3034 5420 2667
Size: 367 2386 5363
Fragments arranged by size:
5363 2386 367
DraIII CAC_nnn'GTG
Cuts at: 4267 4267
Size: 8116
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3275 4676 7123 809
Size: 507 1959 1401 2447 1802
Fragments arranged by size:
2447 1959 1802 1401 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3263 3534 4047
Size: 54 1425 50 275 18 1433 271 513
Cuts at: 4047 5070 5563 6452 8081 8
Size: 1023 493 889 1629 43
Fragments arranged by size:
1629 1433 1425 1023 889 513 493 275
271 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5563 1537
Size: 275 3751 4090
Fragments arranged by size:
4090 3751 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6046 1353
Size: 4693 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3068 3398 5264 6703 7077 7223
Size: 1064 138 1479 330 1866 1439 374 146
Cuts at: 7223 387
Size: 1280
Fragments arranged by size:
1866 1479 1439 1280 1064 374 330 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 2099 2360 2572 2591 2706 2755
Size: 393 445 22 261 212 19 115 49
Cuts at: 2755 3176 3532 4172 4571 4955 6179 6776 7563
Size: 421 356 640 399 384 1224 597 787
Cuts at: 7563 7831 1239
Size: 268 1524
Fragments arranged by size:
1524 1224 787 640 597 445 421 399
393 384 356 268 261 212 115 49
22 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3047 3047
Size: 8116
EcoO109I rG'GnC_Cy
Cuts at: 2263 4141 4716 5020 5106 2263
Size: 1878 575 304 86 5273
Fragments arranged by size:
5273 1878 575 304 86
EcoRI G'AATT_C
Cuts at: 2471 4580 5425 5803 2471
Size: 2109 845 378 4784
Fragments arranged by size:
4784 2109 845 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 8116
FspI TGC'GCA
Cuts at: 6622 6622
Size: 8116
HaeII r_GCGC'y
Cuts at: 1678 2559 3225 3638 4232 5340 7263 1678
Size: 881 666 413 594 1108 1923 2531
Fragments arranged by size:
2531 1923 1108 881 666 594 413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 3966 3998 6757
Size: 32 135 32 743 32 1619 32 2759
Cuts at: 6757 6789 6831 6863 1373
Size: 32 42 32 2626
Fragments arranged by size:
2759 2626 1619 743 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 5460 5706 669
Size: 988 281 798 2724 246 3079
Fragments arranged by size:
3079 2724 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 3647 748
Size: 2899 5217
HpaI GTT'AAC
Cuts at: 5706 5706
Size: 8116
KasI G'GCGC_C
Cuts at: 2555 3221 3634 5336 2555
Size: 666 413 1702 5335
Fragments arranged by size:
5335 1702 666 413
KpnI G_GTAC'C
Cuts at: 4411 5441 4411
Size: 1030 7086
MfeI C'AATT_G
Cuts at: 5715 5715
Size: 8116
MluI A'CGCG_T
Cuts at: 7992 7992
Size: 8116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4365 7230 7414 1305
Size: 3060 2865 184 2007
Fragments arranged by size:
3060 2865 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3265 3536 10
Size: 54 1425 343 1433 271 4590
Fragments arranged by size:
4590 1433 1425 343 271 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 3530 4001 4496 4514 4784 4848
Size: 648 1473 891 471 495 18 270 64
Cuts at: 4848 6074 6433 6592 518
Size: 1226 359 159 2042
Fragments arranged by size:
2042 1473 1226 891 648 495 471 359
270 159 64 18
MspA1I CmG'CkG
Cuts at: 1606 2103 2629 2841 4402 4458 4514 4559 4771
Size: 497 526 212 1561 56 56 45 212
Cuts at: 4771 5026 5532 6209 7357 7602 1606
Size: 255 506 677 1148 245 2120
Fragments arranged by size:
2120 1561 1148 677 526 506 497 255
245 212 212 56 56 45
NaeI GCC'GGC
Cuts at: 5070 5279 5070
Size: 209 7907
NarI GG'CG_CC
Cuts at: 2556 3222 3635 5337 2556
Size: 666 413 1702 5335
Fragments arranged by size:
5335 1702 666 413
NcoI C'CATG_G
Cuts at: 513 1065 2004 3432 3574 4209 4491 513
Size: 552 939 1428 142 635 282 4138
Fragments arranged by size:
4138 1428 939 635 552 282 142
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8116
NgoMIV G'CCGG_C
Cuts at: 5068 5277 5068
Size: 209 7907
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8116
NotI GC'GGCC_GC
Cuts at: 5563 5563
Size: 8116
NspI r_CATG'y
Cuts at: 1132 1229 2404 2683 3345 4246 5474 7019 1132
Size: 97 1175 279 662 901 1228 1545 2229
Fragments arranged by size:
2229 1545 1228 1175 901 662 279 97
PasI CC'CwG_GG
Cuts at: 1348 2965 1348
Size: 1617 6499
PciI A'CATG_T
Cuts at: 2679 4242 7015 2679
Size: 1563 2773 3780
Fragments arranged by size:
3780 2773 1563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2434 4864 1108
Size: 1326 2430 4360
Fragments arranged by size:
4360 2430 1326
PfoI T'CCnGG_A
Cuts at: 2568 4173 5076 2568
Size: 1605 903 5608
Fragments arranged by size:
5608 1605 903
PmeI GTTT'AAAC
Cuts at: 5420 5420
Size: 8116
PmlI CAC'GTG
Cuts at: 3418 4264 3418
Size: 846 7270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6185 6217 7698 7730 6185
Size: 32 1481 32 6571
Fragments arranged by size:
6571 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2263 4141 4716 5020 2263
Size: 1878 575 304 5359
Fragments arranged by size:
5359 1878 575 304
PshAI GACnn'nnGTC
Cuts at: 1613 5222 1613
Size: 3609 4507
PsiI TTA'TAA
Cuts at: 5686 5686
Size: 8116
PspOMI G'GGCC_C
Cuts at: 5105 5105
Size: 8116
PspXI vC'TCGA_Gb
Cuts at: 4834 4834
Size: 8116
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5039 5071 5039
Size: 32 8084
PstI C_TGCA'G
Cuts at: 830 2310 4555 4914 5031 5189 5211 5468 830
Size: 1480 2245 359 117 158 22 257 3478
Fragments arranged by size:
3478 2245 1480 359 257 158 117 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8116
PvuII CAG'CTG
Cuts at: 2629 4514 4771 2629
Size: 1885 257 5974
Fragments arranged by size:
5974 1885 257
RsrII CG'GwC_CG
Cuts at: 3055 3055
Size: 8116
SacII CC_GC'GG
Cuts at: 2842 4560 2842
Size: 1718 6398
SalI G'TCGA_C
Cuts at: 1936 5458 1936
Size: 3522 4594
SbfI CC_TGCA'GG
Cuts at: 5468 5468
Size: 8116
ScaI AGT'ACT
Cuts at: 6364 8076 6364
Size: 1712 6404
SexAI A'CCwGG_T
Cuts at: 4898 4898
Size: 8116
SfcI C'TryA_G
Cuts at: 826 1045 2306 4551 4910 5027 5185 5207 5464
Size: 219 1261 2245 359 117 158 22 257
Cuts at: 5464 6599 7280 7471 826
Size: 1135 681 191 1471
Fragments arranged by size:
2245 1471 1261 1135 681 359 257 219
191 158 117 22
SfoI GGC'GCC
Cuts at: 2557 3223 3636 5338 2557
Size: 666 413 1702 5335
Fragments arranged by size:
5335 1702 666 413
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8116
SgrAI Cr'CCGG_yG
Cuts at: 1700 5277 1700
Size: 3577 4539
SmaI CCC'GGG
Cuts at: 1872 3965 4292 5443 1872
Size: 2093 327 1151 4545
Fragments arranged by size:
4545 2093 1151 327
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4834 6220 7121 7383 820
Size: 197 926 2891 1386 901 262 1553
Fragments arranged by size:
2891 1553 1386 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5907 493
Size: 5414 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8116
SphI G_CATG'C
Cuts at: 1132 3345 5474 1132
Size: 2213 2129 3774
Fragments arranged by size:
3774 2213 2129
SspI AAT'ATT
Cuts at: 5 52 2747 6040 5
Size: 47 2695 3293 2081
Fragments arranged by size:
3293 2695 2081 47
StuI AGG'CCT
Cuts at: 1517 3473 4014 4418 1517
Size: 1956 541 404 5215
Fragments arranged by size:
5215 1956 541 404
StyI C'CwwG_G
Cuts at: 513 1065 2004 2871 3432 3574 4209 4491 5555
Size: 552 939 867 561 142 635 282 1064
Cuts at: 5555 513
Size: 3074
Fragments arranged by size:
3074 1064 939 867 635 561 552 282
142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2497 3072 3404 3578 4047 6143 6160
Size: 1255 917 575 332 174 469 2096 17
Cuts at: 6160 6313 6498 6943 325
Size: 153 185 445 1498
Fragments arranged by size:
2096 1498 1255 917 575 469 445 332
185 174 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2316 2643 3723
Size: 274 80 33 51 1443 339 327 1080
Cuts at: 3723 6362 8074 96
Size: 2639 1712 138
Fragments arranged by size:
2639 1712 1443 1080 339 327 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4622 4654 8076 8108 4622
Size: 32 3422 32 4630
Fragments arranged by size:
4630 3422 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3008 1316
Size: 1692 6424
XbaI T'CTAG_A
Cuts at: 5452 5452
Size: 8116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 2736 2790 5092 2221
Size: 515 54 2302 5245
Fragments arranged by size:
5245 2302 515 54
XhoI C'TCGA_G
Cuts at: 1943 4834 1943
Size: 2891 5225
XmaI C'CCGG_G
Cuts at: 1870 3963 4290 5441 1870
Size: 2093 327 1151 4545
Fragments arranged by size:
4545 2093 1151 327
XmnI GAAnn'nnTTC
Cuts at: 2978 3161 6245 2978
Size: 183 3084 4849
Fragments arranged by size:
4849 3084 183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5924 276
Size: 53 83 186 936 4390 2468
Fragments arranged by size:
4390 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
EcoICRI EcoRV FseI FspAI NruI NsiI PacI SacI
SanDI SapI SfiI SrfI SwaI