Restriction map for
pFN21AE5294
(Circular) (Six-base) MAPSORT of: pFN21AE5294.seq Check: 2536 from: 1 to: 5368
pFN21AE5294 5368 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2406 3178 278
Size: 53 83 186 936 870 772 2468
Fragments arranged by size:
2468 936 870 772 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2711 1937
Size: 774 4594
Acc65I G'GTAC_C
Cuts at: 2689 2689
Size: 5368
AclI AA'CG_TT
Cuts at: 1563 3495 3868 1563
Size: 1932 373 3063
Fragments arranged by size:
3063 1932 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2041 2351 3431 4815 5083 1632
Size: 409 310 1080 1384 268 1917
Fragments arranged by size:
1917 1384 1080 409 310 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5171
AflIII A'CryG_T
Cuts at: 1203 4267 5244 1203
Size: 3064 977 1327
Fragments arranged by size:
3064 1327 977
AgeI A'CCGG_T
Cuts at: 4199 4199
Size: 5368
AhdI GACnn_n'nnGTC
Cuts at: 4097 4097
Size: 5368
AleI CACnn'nnGTG
Cuts at: 1166 2378 1166
Size: 1212 4156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5368
AlwNI CAG_nnn'CTG
Cuts at: 2603 3111 4683 2603
Size: 508 1572 3288
Fragments arranged by size:
3288 1572 508
ApaLI G'TGCA_C
Cuts at: 3425 4581 3425
Size: 1156 4212
ApoI r'AATT_y
Cuts at: 1493 2032 2237 2659 2677 2903 3055 5164 1493
Size: 539 205 422 18 226 152 2109 1697
Fragments arranged by size:
2109 1697 539 422 226 205 152 18
AseI AT'TA_AT
Cuts at: 160 1032 3922 160
Size: 872 2890 1606
Fragments arranged by size:
2890 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 2693 1870
Size: 73 289 461 4545
Fragments arranged by size:
4545 461 289 73
BamHI G'GATC_C
Cuts at: 2698 3065 2698
Size: 367 5001
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2217 2689 4144 618
Size: 325 217 627 430 472 1455 1842
Fragments arranged by size:
1842 1455 627 472 430 325 217
BanII G_rGCy'C
Cuts at: 1391 2301 2687 1391
Size: 910 386 4072
Fragments arranged by size:
4072 910 386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2451 928
Size: 971 552 3845
Fragments arranged by size:
3845 971 552
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3559 3593 1903
Size: 34 1622 34 3678
Fragments arranged by size:
3678 1622 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3260 4470 3260
Size: 1210 4158
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3979 136
Size: 107 122 71 631 605 2307 1525
Fragments arranged by size:
2307 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5363 5363
Size: 5368
BlpI GC'TnA_GC
Cuts at: 2192 2785 2192
Size: 593 4775
Bme1580I G_kGCm'C
Cuts at: 1790 3429 4585 1790
Size: 1639 1156 2573
Fragments arranged by size:
2573 1639 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5368
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3099 4057 442
Size: 416 53 83 399 24 1682 958 1753
Fragments arranged by size:
1753 1682 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4028 1239
Size: 2789 2579
Bpu10I CC'TnA_GC
Cuts at: 2295 2295
Size: 5368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3493 4358 4656 3493
Size: 865 298 4205
Fragments arranged by size:
4205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4031 882
Size: 617 2532 2219
Fragments arranged by size:
2532 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3159 493
Size: 711 1955 2702
Fragments arranged by size:
2702 1955 711
BsaBI GATnn'nnATC
Cuts at: 2343 5362 2343
Size: 3019 2349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2403 3175 3557 4192
Size: 53 83 186 936 870 772 382 635
Cuts at: 4192 275
Size: 1451
Fragments arranged by size:
1451 936 870 772 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2253 2454 3801 4199 4473 4620 5031 2253
Size: 201 1347 398 274 147 411 2590
Fragments arranged by size:
2590 1347 411 398 274 201 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2417 2447 1178
Size: 30 165 30 1014 30 4099
Fragments arranged by size:
4099 1014 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 1186
Size: 1252 4116
BseYI C'CCAG_C
Cuts at: 4571 5067 4571
Size: 496 4872
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2540 1163
Size: 573 804 3991
Fragments arranged by size:
3991 804 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2818 3579 4607 1540
Size: 275 186 817 761 1028 2301
Fragments arranged by size:
2301 1028 817 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2198 2301 3429 3514 4585 2198
Size: 103 1128 85 1071 2981
Fragments arranged by size:
2981 1128 1071 103 85
BsmI GAATG_Cn'
Cuts at: 2879 2972 2879
Size: 93 5275
BsmBI CGTCTCn'nnnn_
Cuts at: 2394 2394
Size: 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2301 2687 3429 3514 4585 1391
Size: 399 408 103 386 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 386 103 85
BspEI T'CCGG_A
Cuts at: 2253 5031 2253
Size: 2778 2590
BspHI T'CATG_A
Cuts at: 2335 3257 4987 2335
Size: 922 1730 2716
Fragments arranged by size:
2716 1730 922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2615 2723 844
Size: 1771 108 3489
Fragments arranged by size:
3489 1771 108
BsrBI CCG'CTC
Cuts at: 1394 1823 3255 1394
Size: 429 1432 3507
Fragments arranged by size:
3507 1432 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2626 3863 4037 65
Size: 1183 1378 1237 174 1396
Fragments arranged by size:
1396 1378 1237 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2261 4012 4199 1700
Size: 110 451 1751 187 2869
Fragments arranged by size:
2869 1751 451 187 110
BsrGI T'GTAC_A
Cuts at: 96 2076 96
Size: 1980 3388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5368
BssSI C'ACGA_G
Cuts at: 2050 3428 4440 2050
Size: 1378 1012 2978
Fragments arranged by size:
2978 1378 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5368
BstBI TT'CG_AA
Cuts at: 4172 4172
Size: 5368
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5368
BstXI CCAn_nnnn'nTGG
Cuts at: 2380 2380
Size: 5368
BstYI r'GATC_y
Cuts at: 1972 2698 3065 3450 3467 4919 5005 5017 5363
Size: 726 367 385 17 1452 86 12 346
Cuts at: 5363 1972
Size: 1977
Fragments arranged by size:
1977 1452 726 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4816
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4629
Fragments arranged by size:
4629 683 56
BtsI GCAGTG_nn'
Cuts at: 2884 3678 3698 2884
Size: 794 20 4554
Fragments arranged by size:
4554 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5333
DraI TTT'AAA
Cuts at: 2672 2672
Size: 5368
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4375 809
Size: 507 3059 1802
Fragments arranged by size:
3059 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2274 2815 3704
Size: 54 1425 50 275 18 444 541 889
Cuts at: 3704 5333 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 541 444 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2815 1537
Size: 275 1003 4090
Fragments arranged by size:
4090 1003 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3298 1353
Size: 1945 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3955 4329 4475 387
Size: 1064 138 2366 374 146 1280
Fragments arranged by size:
2366 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2299 2299
Size: 5368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2351 3431 4028 4815 5083 1239
Size: 393 409 310 1080 597 787 268 1524
Fragments arranged by size:
1524 1080 787 597 409 393 310 268
EcoO109I rG'GnC_Cy
Cuts at: 2248 2248
Size: 5368
EcoRI G'AATT_C
Cuts at: 2237 2677 3055 2237
Size: 440 378 4550
Fragments arranged by size:
4550 440 378
FspI TGC'GCA
Cuts at: 3874 3874
Size: 5368
HaeII r_GCGC'y
Cuts at: 1678 4515 1678
Size: 2837 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 4009 4041 4083
Size: 32 135 32 725 32 1680 32 42
Cuts at: 4083 4115 1373
Size: 32 2626
Fragments arranged by size:
2626 1680 725 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2436 2595 2712 2958 669
Size: 988 281 498 159 117 246 3079
Fragments arranged by size:
3079 988 498 281 246 159 117
HindIII A'AGCT_T
Cuts at: 748 2387 748
Size: 1639 3729
HpaI GTT'AAC
Cuts at: 2595 2958 2595
Size: 363 5005
KpnI G_GTAC'C
Cuts at: 2693 2693
Size: 5368
MfeI C'AATT_G
Cuts at: 2439 2967 2439
Size: 528 4840
MluI A'CGCG_T
Cuts at: 5244 5244
Size: 5368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2207 2374 4482 4666 1305
Size: 902 167 2108 184 2007
Fragments arranged by size:
2108 2007 902 184 167
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3546
Fragments arranged by size:
3546 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2378 3326 3685 3844 518
Size: 648 918 294 948 359 159 2042
Fragments arranged by size:
2042 948 918 648 359 294 159
MspA1I CmG'CkG
Cuts at: 1606 2784 3461 4609 4854 1606
Size: 1178 677 1148 245 2120
Fragments arranged by size:
2120 1178 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4816
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5368
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5368
NotI GC'GGCC_GC
Cuts at: 2815 2815
Size: 5368
NspI r_CATG'y
Cuts at: 1132 1229 2173 2726 4271 1132
Size: 97 944 553 1545 2229
Fragments arranged by size:
2229 1545 944 553 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5368
PciI A'CATG_T
Cuts at: 4267 4267
Size: 5368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5368
PfoI T'CCnGG_A
Cuts at: 2162 2162
Size: 5368
PmeI GTTT'AAAC
Cuts at: 2672 2672
Size: 5368
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 4950 4982 3437
Size: 32 1481 32 3823
Fragments arranged by size:
3823 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5368
PsiI TTA'TAA
Cuts at: 2938 2938
Size: 5368
PstI C_TGCA'G
Cuts at: 830 2125 2470 2720 830
Size: 1295 345 250 3478
Fragments arranged by size:
3478 1295 345 250
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5368
SacI G_AGCT'C
Cuts at: 2301 2301
Size: 5368
SalI G'TCGA_C
Cuts at: 1936 2710 1936
Size: 774 4594
SbfI CC_TGCA'GG
Cuts at: 2720 2720
Size: 5368
ScaI AGT'ACT
Cuts at: 3616 5328 3616
Size: 1712 3656
SfcI C'TryA_G
Cuts at: 826 1045 2121 2466 2716 3851 4532 4723 826
Size: 219 1076 345 250 1135 681 191 1471
Fragments arranged by size:
1471 1135 1076 681 345 250 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5368
SmaI CCC'GGG
Cuts at: 1872 2695 1872
Size: 823 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3472 4373 4635 820
Size: 197 926 1529 901 262 1553
Fragments arranged by size:
1553 1529 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3159 493
Size: 2666 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5368
SphI G_CATG'C
Cuts at: 1132 2726 1132
Size: 1594 3774
SspI AAT'ATT
Cuts at: 5 52 3292 5
Size: 47 3240 2081
Fragments arranged by size:
3240 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5368
StyI C'CwwG_G
Cuts at: 513 1065 2199 2221 2807 513
Size: 552 1134 22 586 3074
Fragments arranged by size:
3074 1134 586 552 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3395 3412 3565 3750 4195 325
Size: 1255 1815 17 153 185 445 1498
Fragments arranged by size:
1815 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2076 3614 5326
Size: 274 80 33 51 1443 99 1538 1712
Cuts at: 5326 96
Size: 138
Fragments arranged by size:
1712 1538 1443 274 138 99 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5328 5360 5328
Size: 32 5336
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5368
XbaI T'CTAG_A
Cuts at: 2704 2704
Size: 5368
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5368
XmaI C'CCGG_G
Cuts at: 1870 2693 1870
Size: 823 4545
XmnI GAAnn'nnTTC
Cuts at: 3497 3497
Size: 5368
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2404 3176 276
Size: 53 83 186 936 870 772 2468
Fragments arranged by size:
2468 936 870 772 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI