Restriction map for
pFN21AE5286
(Circular) (Six-base) MAPSORT of: pFN21AE5286.seq Check: 4782 from: 1 to: 5401
pFN21AE5286 5401 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3211 278
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3464 4848 5116 1632
Size: 1832 1384 268 1917
Fragments arranged by size:
1917 1832 1384 268
AfeI AGC'GCT
Cuts at: 2169 2169
Size: 5401
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5204
AflIII A'CryG_T
Cuts at: 1203 4300 5277 1203
Size: 3097 977 1327
Fragments arranged by size:
3097 1327 977
AgeI A'CCGG_T
Cuts at: 4232 4232
Size: 5401
AhdI GACnn_n'nnGTC
Cuts at: 4130 4130
Size: 5401
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2054 2331 1184
Size: 870 277 4254
Fragments arranged by size:
4254 870 277
AlwNI CAG_nnn'CTG
Cuts at: 2186 2539 3144 4716 2186
Size: 353 605 1572 2871
Fragments arranged by size:
2871 1572 605 353
ApaI G_GGCC'C
Cuts at: 2422 2485 2422
Size: 63 5338
ApaLI G'TGCA_C
Cuts at: 2306 3458 4614 2306
Size: 1152 1156 3093
Fragments arranged by size:
3093 1156 1152
ApoI r'AATT_y
Cuts at: 1493 2710 2936 3088 5197 1493
Size: 1217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2504 2618 2726 1870
Size: 73 561 114 108 4545
Fragments arranged by size:
4545 561 114 108 73
BamHI G'GATC_C
Cuts at: 2731 3098 2731
Size: 367 5034
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2579 2722 4177 618
Size: 325 217 627 243 549 143 1455 1842
Fragments arranged by size:
1842 1455 627 549 325 243 217 143
BanII G_rGCy'C
Cuts at: 1391 2351 2422 2462 2485 2720 1391
Size: 960 71 40 23 235 4072
Fragments arranged by size:
4072 960 235 71 40 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2638 2667 928
Size: 971 739 29 3662
Fragments arranged by size:
3662 971 739 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3592 3626 1903
Size: 34 50 34 1571 34 3678
Fragments arranged by size:
3678 1571 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4503 3293
Size: 1210 4191
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4012 136
Size: 107 122 71 631 605 2340 1525
Fragments arranged by size:
2340 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2345 2818 2345
Size: 473 4928
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2422 2485 2582 3462 4618 1790
Size: 520 112 63 97 880 1156 2573
Fragments arranged by size:
2573 1156 880 520 112 97 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5401
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2534 2556 3132
Size: 416 53 83 399 24 1117 22 576
Cuts at: 3132 4090 442
Size: 958 1753
Fragments arranged by size:
1753 1117 958 576 416 399 83 53
24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2495 2615 2659 4061 1239
Size: 1013 243 120 44 1402 2579
Fragments arranged by size:
2579 1402 1013 243 120 44
Bpu10I CC'TnA_GC
Cuts at: 2174 2174
Size: 5401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3526 4391 4689 3526
Size: 865 298 4238
Fragments arranged by size:
4238 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2685 4064 882
Size: 617 1186 1379 2219
Fragments arranged by size:
2219 1379 1186 617
BsaAI yAC'GTr
Cuts at: 493 1204 3192 493
Size: 711 1988 2702
Fragments arranged by size:
2702 1988 711
BsaBI GATnn'nnATC
Cuts at: 5395 5395
Size: 5401
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3208 3590 4225 275
Size: 53 83 186 936 1675 382 635 1451
Fragments arranged by size:
1675 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3834 4232 4506 4653 5064 3834
Size: 398 274 147 411 4171
Fragments arranged by size:
4171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5176
Fragments arranged by size:
5176 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2612 1186
Size: 1426 3975
BseYI C'CCAG_C
Cuts at: 2008 2128 4604 5100 2008
Size: 120 2476 496 2309
Fragments arranged by size:
2476 2309 496 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2238 2371 1163
Size: 573 502 133 4193
Fragments arranged by size:
4193 573 502 133
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2086 2851 3612 4640 1540
Size: 275 186 85 765 761 1028 2301
Fragments arranged by size:
2301 1028 765 761 275 186 85
BsiHKAI G_wGCw'C
Cuts at: 2310 2351 2625 3462 3547 4618 2310
Size: 41 274 837 85 1071 3093
Fragments arranged by size:
3093 1071 837 274 85 41
BsmI GAATG_Cn'
Cuts at: 2912 3005 2912
Size: 93 5308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2351 2422 2462 2485 2582 2625
Size: 399 520 41 71 40 23 97 43
Cuts at: 2625 2720 3462 3547 4618 1391
Size: 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 520 399 97 95 85
71 43 41 40 23
BspEI T'CCGG_A
Cuts at: 5064 5064
Size: 5401
BspHI T'CATG_A
Cuts at: 3290 5020 3290
Size: 1730 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2673 2756 844
Size: 1307 522 83 3489
Fragments arranged by size:
3489 1307 522 83
BsrBI CCG'CTC
Cuts at: 1394 1823 2563 3288 1394
Size: 429 740 725 3507
Fragments arranged by size:
3507 740 725 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3896 4070 65
Size: 1183 2648 174 1396
Fragments arranged by size:
2648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 4045 4232 1700
Size: 110 217 2018 187 2869
Fragments arranged by size:
2869 2018 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5401
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5401
BssSI C'ACGA_G
Cuts at: 3461 4473 3461
Size: 1012 4389
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5401
BstBI TT'CG_AA
Cuts at: 4205 4205
Size: 5401
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5401
BstYI r'GATC_y
Cuts at: 1972 2199 2731 3098 3483 3500 4952 5038 5050
Size: 227 532 367 385 17 1452 86 12
Cuts at: 5050 5396 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 532 385 367 346 227 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2123 2123
Size: 5401
BtgI C'CryG_G
Cuts at: 513 1065 2149 2493 513
Size: 552 1084 344 3421
Fragments arranged by size:
3421 1084 552 344
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4662
Fragments arranged by size:
4662 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3711 3731 2917
Size: 794 20 4587
Fragments arranged by size:
4587 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5366
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4408 809
Size: 507 3092 1802
Fragments arranged by size:
3092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2068 2433 2848
Size: 54 1425 50 275 18 238 365 415
Cuts at: 2848 3737 5366 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 415 365 275 238 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2848 1537
Size: 275 1036 4090
Fragments arranged by size:
4090 1036 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3331 1353
Size: 1978 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3988 4362 4508 387
Size: 1064 138 2399 374 146 1280
Fragments arranged by size:
2399 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2349 2349
Size: 5401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2495 2615 2659 3464 4061 4848
Size: 393 620 243 120 44 805 597 787
Cuts at: 4848 5116 1239
Size: 268 1524
Fragments arranged by size:
1524 805 787 620 597 393 268 243
120 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 2597
Size: 5401
EcoO109I rG'GnC_Cy
Cuts at: 2247 2327 2482 2247
Size: 80 155 5166
Fragments arranged by size:
5166 155 80
EcoRI G'AATT_C
Cuts at: 2710 3088 2710
Size: 378 5023
FspI TGC'GCA
Cuts at: 3907 3907
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 2171 4548 1678
Size: 493 2377 2531
Fragments arranged by size:
2531 2377 493
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4042 4074 4116 4148 1373
Size: 32 135 32 2470 32 42 32 2626
Fragments arranged by size:
2626 2470 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 2587 748
Size: 1839 3562
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 3000 3000
Size: 5401
MluI A'CGCG_T
Cuts at: 5277 5277
Size: 5401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2656 4515 4699 1305
Size: 1351 1859 184 2007
Fragments arranged by size:
2007 1859 1351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 10
Size: 54 1425 343 603 2976
Fragments arranged by size:
2976 1425 603 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2280 2337 3359 3718 3877 518
Size: 648 870 244 57 1022 359 159 2042
Fragments arranged by size:
2042 1022 870 648 359 244 159 57
MspA1I CmG'CkG
Cuts at: 1606 2264 2539 2817 3494 4642 4887 1606
Size: 658 275 278 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 658 278 275 245
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 5401
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4849
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5401
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 5401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2759 4304 1132
Size: 97 1530 1545 2229
Fragments arranged by size:
2229 1545 1530 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5401
PciI A'CATG_T
Cuts at: 4300 4300
Size: 5401
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5401
PfoI T'CCnGG_A
Cuts at: 2211 2592 2211
Size: 381 5020
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2247 2327 2247
Size: 80 5321
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5401
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PspOMI G'GGCC_C
Cuts at: 2418 2481 2418
Size: 63 5338
PstI C_TGCA'G
Cuts at: 830 2535 2753 830
Size: 1705 218 3478
Fragments arranged by size:
3478 1705 218
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
PvuII CAG'CTG
Cuts at: 2264 2264
Size: 5401
SacI G_AGCT'C
Cuts at: 2351 2351
Size: 5401
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SanDI GG'GwC_CC
Cuts at: 2247 2327 2247
Size: 80 5321
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 3649 5361 3649
Size: 1712 3689
SfcI C'TryA_G
Cuts at: 826 1045 2531 2749 3884 4565 4756 826
Size: 219 1486 218 1135 681 191 1471
Fragments arranged by size:
1486 1471 1135 681 219 218 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2728 1872
Size: 856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 3505 4406 4668 820
Size: 197 926 675 887 901 262 1553
Fragments arranged by size:
1553 926 901 887 675 262 197
SnaBI TAC'GTA
Cuts at: 493 3192 493
Size: 2699 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2759 1132
Size: 1627 3774
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 2099 2237 1517
Size: 582 138 4681
Fragments arranged by size:
4681 582 138
StyI C'CwwG_G
Cuts at: 513 1065 2059 2840 513
Size: 552 994 781 3074
Fragments arranged by size:
3074 994 781 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2272 3428 3445 3598 3783 4228 325
Size: 1255 692 1156 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1156 692 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3647 5359 96
Size: 274 80 33 51 1443 1670 1712 138
Fragments arranged by size:
1712 1670 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5361 5393 5361
Size: 32 5369
Tth111I GACn'n_nGTC
Cuts at: 1316 2642 1316
Size: 1326 4075
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XhoI C'TCGA_G
Cuts at: 1943 2618 1943
Size: 675 4726
XmaI C'CCGG_G
Cuts at: 1870 2726 1870
Size: 856 4545
XmnI GAAnn'nnTTC
Cuts at: 3530 3530
Size: 5401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3209 276
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI