Restriction map for
pFN21AE5284
(Circular) (Six-base) MAPSORT of: pFN21AE5284.seq Check: 9347 from: 1 to: 5335
pFN21AE5284 5335 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2518 2518
Size: 5335
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 1937
Size: 741 4594
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 3398 4782 5050 1632
Size: 539 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 539 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 4234 5211 1203
Size: 3031 977 1327
Fragments arranged by size:
3031 1327 977
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 4064 4064
Size: 5335
AleI CACnn'nnGTG
Cuts at: 1166 2310 1166
Size: 1144 4191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5335
AlwNI CAG_nnn'CTG
Cuts at: 2251 2476 3078 4650 2251
Size: 225 602 1572 2936
Fragments arranged by size:
2936 1572 602 225
ApaI G_GGCC'C
Cuts at: 2488 2518 2488
Size: 30 5305
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2644 2870 3022 5131 1493
Size: 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 2487 2660 1870
Size: 73 191 353 173 4545
Fragments arranged by size:
4545 353 191 173 73
BamHI G'GATC_C
Cuts at: 2665 3032 2665
Size: 367 4968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2656 4111 618
Size: 325 217 627 869 1455 1842
Fragments arranged by size:
1842 1455 869 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2488 2518 2654 1391
Size: 1097 30 136 4072
Fragments arranged by size:
4072 1097 136 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2159 928
Size: 971 260 4104
Fragments arranged by size:
4104 971 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2125 2159 2569 2603 3526 3560 1903
Size: 34 188 34 410 34 923 34 3678
Fragments arranged by size:
3678 923 410 188 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3946 136
Size: 107 122 71 631 605 2274 1525
Fragments arranged by size:
2274 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2124 2383 2397 5330 2124
Size: 259 14 2933 2129
Fragments arranged by size:
2933 2129 259 14
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 2488 2518 3396 4552 1790
Size: 698 30 878 1156 2573
Fragments arranged by size:
2573 1156 878 698 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3066 4024 442
Size: 416 53 83 399 24 1649 958 1753
Fragments arranged by size:
1753 1649 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2273 3995 1239
Size: 968 66 1722 2579
Fragments arranged by size:
2579 1722 968 66
Bpu10I CC'TnA_GC
Cuts at: 2493 2493
Size: 5335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 3460 4325 4623 2507
Size: 953 865 298 3219
Fragments arranged by size:
3219 953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3998 882
Size: 617 2499 2219
Fragments arranged by size:
2499 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3126 493
Size: 711 1922 2702
Fragments arranged by size:
2702 1922 711
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2065 3142 3524 4159
Size: 53 83 186 936 532 1077 382 635
Cuts at: 4159 275
Size: 1451
Fragments arranged by size:
1451 1077 936 635 532 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3768 4166 4440 4587 4998 3768
Size: 398 274 147 411 4105
Fragments arranged by size:
4105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5110
Fragments arranged by size:
5110 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 2216 1186
Size: 876 154 4305
Fragments arranged by size:
4305 876 154
BseYI C'CCAG_C
Cuts at: 2266 2479 4538 5034 2266
Size: 213 2059 496 2567
Fragments arranged by size:
2567 2059 496 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2031 1163
Size: 573 295 4467
Fragments arranged by size:
4467 573 295
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2785 3546 4574 1540
Size: 275 186 784 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 3396 3481 4552 2237
Size: 1159 85 1071 3020
Fragments arranged by size:
3020 1159 1071 85
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2488 2518 2654 3396 3481 4552
Size: 399 447 251 30 136 742 85 1071
Cuts at: 4552 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 447 399 251 136 85
30
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 3224 4954 3224
Size: 1730 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 2518 2690 844
Size: 1673 1 172 3489
Fragments arranged by size:
3489 1673 172 1
BsrBI CCG'CTC
Cuts at: 1394 1823 2070 2206 3222 1394
Size: 429 247 136 1016 3507
Fragments arranged by size:
3507 1016 429 247 136
BsrDI GCAATG_nn'
Cuts at: 65 1248 3830 4004 65
Size: 1183 2582 174 1396
Fragments arranged by size:
2582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3979 4166 1700
Size: 110 2169 187 2869
Fragments arranged by size:
2869 2169 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 2224 1925
Size: 299 5036
BssSI C'ACGA_G
Cuts at: 3395 4407 3395
Size: 1012 4323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2241 2476 794
Size: 1447 235 3653
Fragments arranged by size:
3653 1447 235
BstBI TT'CG_AA
Cuts at: 4139 4139
Size: 5335
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5335
BstYI r'GATC_y
Cuts at: 1972 2124 2383 2397 2448 2665 3032 3417 3434
Size: 152 259 14 51 217 367 385 17
Cuts at: 3434 4886 4972 4984 5330 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 259 217 152
86 51 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 513
Size: 552 939 90 3754
Fragments arranged by size:
3754 939 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2037 487
Size: 683 56 811 3785
Fragments arranged by size:
3785 811 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3645 3665 2851
Size: 794 20 4521
Fragments arranged by size:
4521 794 20
ClaI AT'CG_AT
Cuts at: 1554 2589 1554
Size: 1035 4300
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5300
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DraIII CAC_nnn'GTG
Cuts at: 2600 2600
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4342 809
Size: 507 3026 1802
Fragments arranged by size:
3026 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2782 3671
Size: 54 1425 50 275 18 429 523 889
Cuts at: 3671 5300 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 523 429 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 1537
Size: 275 970 4090
Fragments arranged by size:
4090 970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 3265 1353
Size: 972 940 3423
Fragments arranged by size:
3423 972 940
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2119 2213 3922 4296 4442
Size: 1064 138 435 95 94 1709 374 146
Cuts at: 4442 387
Size: 1280
Fragments arranged by size:
1709 1280 1064 435 374 146 138 95
94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2207 2273 3398 3995 4782 5050
Size: 393 539 36 66 1125 597 787 268
Cuts at: 5050 1239
Size: 1524
Fragments arranged by size:
1524 1125 787 597 539 393 268 66
36
EcoNI CCTnn'n_nnAGG
Cuts at: 2525 2525
Size: 5335
EcoO109I rG'GnC_Cy
Cuts at: 2484 2484
Size: 5335
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 3841 3841
Size: 5335
HaeII r_GCGC'y
Cuts at: 1678 2477 4482 1678
Size: 799 2005 2531
Fragments arranged by size:
2531 2005 799
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2368 2400 2433 2465 3976
Size: 32 135 32 796 32 33 32 1511
Cuts at: 3976 4008 4050 4082 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1511 796 135 42 33 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 2925 669
Size: 988 281 741 246 3079
Fragments arranged by size:
3079 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 4449 4633 1305
Size: 795 2349 184 2007
Fragments arranged by size:
2349 2007 795 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3513
Fragments arranged by size:
3513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2310 3293 3652 3811 518
Size: 648 1144 983 359 159 2042
Fragments arranged by size:
2042 1144 983 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2149 2241 2251 2751 3428 4576 4821 1606
Size: 543 92 10 500 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 543 500 245 92 10
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3844
Fragments arranged by size:
3844 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2782 2782
Size: 5335
NspI r_CATG'y
Cuts at: 1132 1229 2693 4238 1132
Size: 97 1464 1545 2229
Fragments arranged by size:
2229 1545 1464 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5335
PciI A'CATG_T
Cuts at: 4234 4234
Size: 5335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 1108
Size: 1143 4192
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 4917 4949 3404
Size: 32 1481 32 3790
Fragments arranged by size:
3790 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PspOMI G'GGCC_C
Cuts at: 2484 2514 2484
Size: 30 5305
PstI C_TGCA'G
Cuts at: 830 2169 2286 2458 2687 830
Size: 1339 117 172 229 3478
Fragments arranged by size:
3478 1339 229 172 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
PvuII CAG'CTG
Cuts at: 2149 2149
Size: 5335
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SbfI CC_TGCA'GG
Cuts at: 2687 2687
Size: 5335
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SfcI C'TryA_G
Cuts at: 826 1045 2165 2282 2454 2683 3818 4499 4690
Size: 219 1120 117 172 229 1135 681 191
Cuts at: 4690 826
Size: 1471
Fragments arranged by size:
1471 1135 1120 681 229 219 191 172
117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2489 2662 1872
Size: 617 173 4545
Fragments arranged by size:
4545 617 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2134 2522 3439 4340 4602 820
Size: 197 926 191 388 917 901 262 1553
Fragments arranged by size:
1553 926 917 901 388 262 197 191
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2693 1132
Size: 1561 3774
SrfI GCCC'GGGC
Cuts at: 2489 2489
Size: 5335
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5335
StyI C'CwwG_G
Cuts at: 513 1065 2004 2774 513
Size: 552 939 770 3074
Fragments arranged by size:
3074 939 770 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3362 3379 3532 3717 4162 325
Size: 1255 1782 17 153 185 445 1498
Fragments arranged by size:
1782 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3581 5293 96
Size: 274 80 33 51 1443 1604 1712 138
Fragments arranged by size:
1712 1604 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5295 5327 5295
Size: 32 5303
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XhoI C'TCGA_G
Cuts at: 1943 2134 1943
Size: 191 5144
XmaI C'CCGG_G
Cuts at: 1870 2487 2660 1870
Size: 617 173 4545
Fragments arranged by size:
4545 617 173
XmnI GAAnn'nnTTC
Cuts at: 3464 3464
Size: 5335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SwaI
XcmI