Restriction map for
pFN21AE5278
(Circular) (Six-base) MAPSORT of: pFN21AE5278.seq Check: 4767 from: 1 to: 5410
pFN21AE5278 5410 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3220 278
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 1937
Size: 816 4594
Acc65I G'GTAC_C
Cuts at: 2185 2731 2185
Size: 546 4864
AclI AA'CG_TT
Cuts at: 1563 3537 3910 1563
Size: 1974 373 3063
Fragments arranged by size:
3063 1974 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3473 4857 5125 1632
Size: 1841 1384 268 1917
Fragments arranged by size:
1917 1841 1384 268
AfeI AGC'GCT
Cuts at: 2462 2525 2462
Size: 63 5347
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5213
AflIII A'CryG_T
Cuts at: 1203 4309 5286 1203
Size: 3106 977 1327
Fragments arranged by size:
3106 1327 977
AgeI A'CCGG_T
Cuts at: 4241 4241
Size: 5410
AhdI GACnn_n'nnGTC
Cuts at: 4139 4139
Size: 5410
AleI CACnn'nnGTG
Cuts at: 1166 2297 1166
Size: 1131 4279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2286 1184
Size: 1102 4308
AlwNI CAG_nnn'CTG
Cuts at: 2134 2602 3153 4725 2134
Size: 468 551 1572 2819
Fragments arranged by size:
2819 1572 551 468
ApaLI G'TGCA_C
Cuts at: 2261 3467 4623 2261
Size: 1206 1156 3048
Fragments arranged by size:
3048 1206 1156
ApoI r'AATT_y
Cuts at: 1493 2719 2945 3097 5206 1493
Size: 1226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3964 160
Size: 872 2932 1606
Fragments arranged by size:
2932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
AvaI C'yCGr_G
Cuts at: 1870 1943 2619 2735 1870
Size: 73 676 116 4545
Fragments arranged by size:
4545 676 116 73
BamHI G'GATC_C
Cuts at: 2740 3107 2740
Size: 367 5043
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2185 2605 2731 4186 618
Size: 325 217 627 398 420 126 1455 1842
Fragments arranged by size:
1842 1455 627 420 398 325 217 126
BanII G_rGCy'C
Cuts at: 1391 2477 2540 2729 1391
Size: 1086 63 189 4072
Fragments arranged by size:
4072 1086 189 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2149 2183 3601 3635 1903
Size: 34 212 34 1418 34 3678
Fragments arranged by size:
3678 1418 212 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3302 4512 3302
Size: 1210 4200
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5410
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 2535 4021
Size: 107 122 71 631 605 688 175 1486
Cuts at: 4021 136
Size: 1525
Fragments arranged by size:
1525 1486 688 631 605 175 122 107
71
BglII A'GATC_T
Cuts at: 5405 5405
Size: 5410
BlpI GC'TnA_GC
Cuts at: 2827 2827
Size: 5410
Bme1580I G_kGCm'C
Cuts at: 1790 2265 3471 4627 1790
Size: 475 1206 1156 2573
Fragments arranged by size:
2573 1206 1156 475
BmgBI CAC'GTC
Cuts at: 1318 2344 1318
Size: 1026 4384
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3141 4099 442
Size: 416 53 83 399 24 1724 958 1753
Fragments arranged by size:
1753 1724 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 4070 1239
Size: 1235 1596 2579
Fragments arranged by size:
2579 1596 1235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3535 4400 4698 3535
Size: 865 298 4247
Fragments arranged by size:
4247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4073 882
Size: 617 2574 2219
Fragments arranged by size:
2574 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3201 493
Size: 711 1997 2702
Fragments arranged by size:
2702 1997 711
BsaBI GATnn'nnATC
Cuts at: 5404 5404
Size: 5410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3217 3599 4234 275
Size: 53 83 186 936 1684 382 635 1451
Fragments arranged by size:
1684 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3843 4241 4515 4662 5073 3843
Size: 398 274 147 411 4180
Fragments arranged by size:
4180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5185
Fragments arranged by size:
5185 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 2504 2507 2545 1186
Size: 1285 33 3 38 4051
Fragments arranged by size:
4051 1285 38 33 3
BseYI C'CCAG_C
Cuts at: 2080 2127 2421 4613 5109 2080
Size: 47 294 2192 496 2381
Fragments arranged by size:
2381 2192 496 294 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4837
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2169 2636 2860 3621 4649 1540
Size: 275 186 168 467 224 761 1028 2301
Fragments arranged by size:
2301 1028 761 467 275 224 186 168
BsiHKAI G_wGCw'C
Cuts at: 2265 3471 3556 4627 2265
Size: 1206 85 1071 3048
Fragments arranged by size:
3048 1206 1071 85
BsmI GAATG_Cn'
Cuts at: 2921 3014 2921
Size: 93 5317
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2477 2540 2729 3471 3556 4627
Size: 399 475 212 63 189 742 85 1071
Cuts at: 4627 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 475 399 212 189 85
63
BspEI T'CCGG_A
Cuts at: 5073 5073
Size: 5410
BspHI T'CATG_A
Cuts at: 3299 5029 3299
Size: 1730 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2765 844
Size: 1921 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3297 1394
Size: 429 1474 3507
Fragments arranged by size:
3507 1474 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2017 3905 4079 65
Size: 1183 769 1888 174 1396
Fragments arranged by size:
1888 1396 1183 769 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2445 2529 2631 4054 4241 1700
Size: 110 635 84 102 1423 187 2869
Fragments arranged by size:
2869 1423 635 187 110 102 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5410
BssHII G'CGCG_C
Cuts at: 1925 2500 2502 2651 1925
Size: 575 2 149 4684
Fragments arranged by size:
4684 575 149 2
BssSI C'ACGA_G
Cuts at: 3470 4482 3470
Size: 1012 4398
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2526 794
Size: 1732 3678
BstBI TT'CG_AA
Cuts at: 4214 4214
Size: 5410
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5410
BstXI CCAn_nnnn'nTGG
Cuts at: 2071 2071
Size: 5410
BstYI r'GATC_y
Cuts at: 1972 2740 3107 3492 3509 4961 5047 5059 5405
Size: 768 367 385 17 1452 86 12 346
Cuts at: 5405 1972
Size: 1977
Fragments arranged by size:
1977 1452 768 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2181 2181
Size: 5410
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3919
Fragments arranged by size:
3919 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2359 487
Size: 683 56 1133 3538
Fragments arranged by size:
3538 1133 683 56
BtsI GCAGTG_nn'
Cuts at: 2227 2263 2926 3720 3740 2227
Size: 36 663 794 20 3897
Fragments arranged by size:
3897 794 663 36 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5375
DraI TTT'AAA
Cuts at: 2714 2714
Size: 5410
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4417 809
Size: 507 3101 1802
Fragments arranged by size:
3101 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2633 2857 3746
Size: 54 1425 50 275 18 803 224 889
Cuts at: 3746 5375 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 803 275 224 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2633 2857 1537
Size: 275 821 224 4090
Fragments arranged by size:
4090 821 275 224
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 3340 1353
Size: 1053 934 3423
Fragments arranged by size:
3423 1053 934
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2548 3997 4371 4517 387
Size: 1064 138 959 1449 374 146 1280
Fragments arranged by size:
1449 1280 1064 959 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2474 3473 4070 4857 5125 1239
Size: 393 842 999 597 787 268 1524
Fragments arranged by size:
1524 999 842 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2719 3097 2719
Size: 378 5032
EcoRV GAT'ATC
Cuts at: 2146 2146
Size: 5410
FspI TGC'GCA
Cuts at: 3916 3916
Size: 5410
HaeII r_GCGC'y
Cuts at: 1678 2464 2527 2602 4557 1678
Size: 786 63 75 1955 2531
Fragments arranged by size:
2531 1955 786 75 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 4051 4083 4125
Size: 32 135 32 456 32 1991 32 42
Cuts at: 4125 4157 1373
Size: 32 2626
Fragments arranged by size:
2626 1991 456 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2754 3000 669
Size: 988 281 816 246 3079
Fragments arranged by size:
3079 988 816 281 246
HindIII A'AGCT_T
Cuts at: 748 2438 748
Size: 1690 3720
HpaI GTT'AAC
Cuts at: 3000 3000
Size: 5410
KpnI G_GTAC'C
Cuts at: 2189 2735 2189
Size: 546 4864
MfeI C'AATT_G
Cuts at: 3009 3009
Size: 5410
MluI A'CGCG_T
Cuts at: 5286 5286
Size: 5410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4524 4708 1305
Size: 3219 184 2007
Fragments arranged by size:
3219 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3588
Fragments arranged by size:
3588 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 3368 3727 3886 518
Size: 648 1131 1071 359 159 2042
Fragments arranged by size:
2042 1131 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2452 2515 2638 2644 2826 3503 4651
Size: 474 372 63 123 6 182 677 1148
Cuts at: 4651 4896 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 474 372 245 182 123
63 6
NaeI GCC'GGC
Cuts at: 2447 2531 2447
Size: 84 5326
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3919
Fragments arranged by size:
3919 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5410
NgoMIV G'CCGG_C
Cuts at: 2445 2529 2445
Size: 84 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5410
NotI GC'GGCC_GC
Cuts at: 2857 2857
Size: 5410
NspI r_CATG'y
Cuts at: 1132 1229 2768 4313 1132
Size: 97 1539 1545 2229
Fragments arranged by size:
2229 1545 1539 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5410
PciI A'CATG_T
Cuts at: 4309 4309
Size: 5410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5410
PfoI T'CCnGG_A
Cuts at: 2032 2032
Size: 5410
PmeI GTTT'AAAC
Cuts at: 2714 2714
Size: 5410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 4992 5024 3479
Size: 32 1481 32 3865
Fragments arranged by size:
3865 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5410
PsiI TTA'TAA
Cuts at: 2980 2980
Size: 5410
PstI C_TGCA'G
Cuts at: 830 2142 2598 2762 830
Size: 1312 456 164 3478
Fragments arranged by size:
3478 1312 456 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5410
PvuII CAG'CTG
Cuts at: 2080 2452 2080
Size: 372 5038
SalI G'TCGA_C
Cuts at: 1936 2752 1936
Size: 816 4594
SbfI CC_TGCA'GG
Cuts at: 2762 2762
Size: 5410
ScaI AGT'ACT
Cuts at: 3658 5370 3658
Size: 1712 3698
SfcI C'TryA_G
Cuts at: 826 1045 2138 2594 2758 3893 4574 4765 826
Size: 219 1093 456 164 1135 681 191 1471
Fragments arranged by size:
1471 1135 1093 681 456 219 191 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5410
SmaI CCC'GGG
Cuts at: 1872 2621 2737 1872
Size: 749 116 4545
Fragments arranged by size:
4545 749 116
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3514 4415 4677 820
Size: 197 926 1571 901 262 1553
Fragments arranged by size:
1571 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3201 493
Size: 2708 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5410
SphI G_CATG'C
Cuts at: 1132 2768 1132
Size: 1636 3774
SspI AAT'ATT
Cuts at: 5 52 3334 5
Size: 47 3282 2081
Fragments arranged by size:
3282 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5410
StyI C'CwwG_G
Cuts at: 513 1065 2004 2022 2849 513
Size: 552 939 18 827 3074
Fragments arranged by size:
3074 939 827 552 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3437 3454 3607 3792 4237 325
Size: 1255 1857 17 153 185 445 1498
Fragments arranged by size:
1857 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3656 5368 96
Size: 274 80 33 51 1443 1679 1712 138
Fragments arranged by size:
1712 1679 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5370 5402 5370
Size: 32 5378
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5410
XbaI T'CTAG_A
Cuts at: 2746 2746
Size: 5410
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5410
XmaI C'CCGG_G
Cuts at: 1870 2619 2735 1870
Size: 749 116 4545
Fragments arranged by size:
4545 749 116
XmnI GAAnn'nnTTC
Cuts at: 3539 3539
Size: 5410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3218 276
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI